The purpose of this study was to compare the effect of pH on the growth kinetics of Listeria monocytogenes Scott A static and agitated broth stored at 4 and 10 degrees C with and without the combination of 1.85% potassium lactate (PL) and 0.13% sodium diacetate (SDA ) (3.3% of a commercial solution of 60%, PURASAL P Opti. Form 4). PH brain heart infusion broth without (control) or with 1.85% PL + 0.13% SDA adjusted to 5.5, 6.0, 6.5, and 7.5. L. monocytogenes Scott A inoculated (at 10 (2) CFU / ml) to the pH-adjusted broth, which is stored at 4 or 10 ° C with or without agitation. At pH 5.5, listeriostatic effects observed for broth containing 1.85% PL + 0.13% SDA at 4 and 10 degrees C both with and without agitation.
At pH 6.0, 1.85% PL + 0.13% SDA completely controlled growth of L. monocytogenes Scott A in broth static at 4 degrees C up to 20 days and significantly slow the growth of pathogens in broth agitated. At 10 degrees C, the growth of L. monocytogenes Scott A was significantly decreased 1.85% PL + 0.13% SDA in broth agitated and unagitated. At pH 6.5, 1.85% PL + 0.13% SDA significantly suppressed the growth of L. monocytogenes Scott A in both 4 degrees C (P <0.001) and 10 degrees C (P <0.01). At pH 7.5, 1.85% PL + 0.13% SDA had a limited effect on the growth of L. monocytogenes Scott A in broth stored at 4 and 10 degrees C. at 4 degrees C, the agitation decreased lag time and increase the rate of growth L. monocytogenes Scott A at all pH tested. Similar but less obvious trend was observed for the broth stored at 10 degrees C. These results suggest that the combination of lactic-diacetate effectively act with low pH and temperature to inhibit the growth of L. monocytogenes Scott A.
A simple HPLC method was developed and validated for the quantification of linezolid in Brain Heart Infusion (BHI) broth. Eperezolid employed as an internal standard and sample pre-treatment procedure is simple. calibration standards ranging from 0.05 to 16 mg / L is within 7.8% accuracy and reproducibility (RSD) of less than 8.3%. Recovery is about 100% at all concentrations examined. Linezolid autosampler insert stable for at least 24 hours at ambient temperature and in BHI for 72 hours at 37 degrees C. This test is quick and ideal for the analysis of large numbers of samples.
Resistance of Listeria monocytogenes F2365 cells to a greater synthetic gastric fluid following the growth in ready-to-eat deli turkey meat than in brain heart infusion broth.
Ready-to-eat (RTE) meat shop has been categorized as high-risk foods for foodborne listeriosis contraction. Several outbreaks of listeriosis has recently been associated with the consumption of RTE deli turkey meat. In this study, we examined whether the growth of Listeria monocytogenes F2365 on commercially prepared RTE deli turkey meat causes listerial cells become more resistant to inactivation by synthetic gastric fluid (SGF). listerial cells grow on the turkey until the end of the logarithmic-early stationary phase were significantly more resistant to SGF at pH 7.0, 5.0, or 3.5 of listerial cells grown in brain heart infusion (BHI) broth. the lower pH in the liquid in the package of turkey than in BHI broth (6.5 vs. 7.5).
Rat anti Human IgG |
40R-1588 |
Fitzgerald |
100 ug |
EUR 318 |
Description: Rat monoclonal Rat anti Human IgG antibody |
Goat anti Human IgA |
20-B9001G000-S0 |
Fitzgerald |
10 ml |
EUR 159.6 |
Description: Goat anti Human IgA antibody |
Goat anti Human IgM |
20-B9022G000-S2 |
Fitzgerald |
10 ml |
EUR 159.6 |
Description: Goat anti Human IgM antibody |
Goat anti Human IgA |
20-S11030GND1-D |
Fitzgerald |
5 mg |
EUR 230.4 |
Description: Goat anti Human IgA antibody |
Goat anti Human IgA |
20-S1103GND1-D0 |
Fitzgerald |
10 ml |
EUR 230.4 |
Description: Goat anti Human IgA antibody |
Goat anti Human IgA |
20-S1111G000-S4 |
Fitzgerald |
10 ml |
EUR 159.6 |
Description: Goat anti Human IgA antibody |
Goat anti Human IgA |
20-S1111G000-V0 |
Fitzgerald |
10 ml |
EUR 159.6 |
Description: Goat anti Human IgA antibody |
Goat anti Human IgA |
20-S1111GOOO-S4 |
Fitzgerald |
10 ml |
EUR 159.6 |
Description: Goat anti Human IgA antibody |
Goat anti Human IgM |
20-S1303GND1-D0 |
Fitzgerald |
10 ml |
EUR 230.4 |
Description: Goat anti Human IgM antibody |
Goat anti Human IgM |
20-S1304GA01 |
Fitzgerald |
5 ml |
EUR 165.6 |
Description: Goat anti Human IgM antibody |
Goat anti Human IgM |
20-S1311G000-S4 |
Fitzgerald |
10 ml |
EUR 159.6 |
Description: Goat anti Human IgM antibody |
Goat anti Human IgM |
20-S1311G000-V0 |
Fitzgerald |
10 ml |
EUR 133.2 |
Description: Goat anti Human IgM antibody |
Goat anti Human IgM |
20-S1311G001-S4 |
Fitzgerald |
10 ml |
EUR 159.6 |
Description: Goat anti Human IgM antibody |
Goat anti Human IgE |
20-S2001G000-S4 |
Fitzgerald |
10 ml |
EUR 152.4 |
Description: Goat anti Human IgE antibody |
Goat anti Human IgE |
20-S2001G000-V0 |
Fitzgerald |
10 ml |
EUR 159.6 |
Description: Goat anti Human IgE antibody |
Goat anti Human IgE |
20-S2001G000-VO |
Fitzgerald |
10 ml |
EUR 159.6 |
Description: Goat anti Human IgE antibody |
Goat anti Human IgE |
20-S2003GND1-D0 |
Fitzgerald |
10 ml |
EUR 230.4 |
Description: Goat anti Human IgE antibody |
Goat anti Human IgM |
20-S4113GN00-D4 |
Fitzgerald |
10 ml |
EUR 230.4 |
Description: Goat anti Human IgM antibody |
Goat anti Human IgM |
20-S5170GND1-D0 |
Fitzgerald |
10 ml |
EUR 230.4 |
Description: Goat anti Human IgM antibody |
Goat anti Human IgA |
20-S5491G000-S4 |
Fitzgerald |
10 ml |
EUR 159.6 |
Description: Goat anti Human IgA antibody |
Goat anti Human IgE |
40-IG10 |
Fitzgerald |
1 ml |
EUR 159.6 |
Description: Goat anti Human IgE antibody |
Goat anti Human IgG |
41C-CB0957 |
Fitzgerald |
5 mg |
EUR 415.2 |
Description: Goat anti Human IgG secondary antibody |
Goat anti Human IgG |
41C-CB0972 |
Fitzgerald |
2 mg |
EUR 316.8 |
Description: Goat anti Human IgG secondary antibody |
Goat anti Human IgM |
41C-CB0993 |
Fitzgerald |
1 mg |
EUR 244.8 |
Description: Goat anti Human IgM secondary antibody |
Goat anti Human IgG |
41R-1026 |
Fitzgerald |
2 mg |
EUR 164.4 |
Description: Goat anti Human IgG secondary antibody |
Goat anti Human IgG |
41R-1027 |
Fitzgerald |
2 mg |
EUR 223.2 |
Description: Goat anti Human IgG secondary antibody |
Goat anti Human IgG |
41R-1028 |
Fitzgerald |
1 mg |
EUR 218.4 |
Description: Goat anti Human IgG secondary antibody |
Goat anti Human IgG |
41R-1029 |
Fitzgerald |
2 mg |
EUR 223.2 |
Description: Goat anti Human IgG secondary antibody |
Goat anti Human IgG |
41R-1030 |
Fitzgerald |
1 mg |
EUR 165.6 |
Description: Goat anti Human IgG secondary antibody |
Goat anti Human IgG |
41R-1031 |
Fitzgerald |
1 mg |
EUR 223.2 |
Description: Goat anti Human IgG secondary antibody |
Goat anti Human IgG |
41R-1032 |
Fitzgerald |
1 mg |
EUR 268.8 |
Description: Goat anti Human IgG secondary antibody |
Goat anti Human IgG |
41R-1033 |
Fitzgerald |
1 mg |
EUR 295.2 |
Description: Goat anti Human IgG secondary antibody |
Goat anti Human IgG |
41R-1034 |
Fitzgerald |
1 mg |
EUR 223.2 |
Description: Goat anti Human IgG secondary antibody |
Rat anti Human IgG1 |
40R-1007 |
Fitzgerald |
100 ug |
EUR 318 |
Description: Rat monoclonal Rat anti Human IgG1 antibody |
Goat anti Human IgG |
41-XG56 |
Fitzgerald |
1 mg |
EUR 121.2 |
Description: Goat anti Human IgG secondary antibody |
Goat anti Human IgE |
70R-IG015 |
Fitzgerald |
1 mg |
EUR 418.8 |
Description: Affinity purified Goat anti Human IgE antibody |
Goat anti Human IgM |
70-B9022GA00-A0 |
Fitzgerald |
5 mg |
EUR 230.4 |
Description: Goat anti Human IgM antibody |
Goat anti Human IgG |
70C-CR6047GAP |
Fitzgerald |
1 mg |
EUR 178.8 |
Description: Affinity purified Goat anti Human IgG antibody |
Goat anti- human IgA |
FNab09895 |
FN Test |
100µg |
EUR 658.5 |
|
Description: Antibody raised against Goat human IgA |
Goat anti- human IgG |
FNab09896 |
FN Test |
100µg |
Ask for price |
|
Description: Antibody raised against Goat human IgG |
Goat anti- human IgM |
FNab09908 |
FN Test |
100µg |
EUR 658.5 |
|
Description: Antibody raised against Goat human IgM |
Goat Anti Human IgG |
E61I00201 |
EnoGene |
1mg |
EUR 328.8 |
Goat Anti Human IgM |
E61I00401 |
EnoGene |
100ug |
EUR 411.6 |
Goat anti human IgA |
E61I01201 |
EnoGene |
1mg |
EUR 733.2 |
Goat anti human IgA |
E61I02102 |
EnoGene |
100ug |
EUR 411.6 |
FITC anti human CD3 |
E16FHF003-025 |
EnoGene |
25 tests |
EUR 89 |
Description: Available in various conjugation types. |
FITC anti human CD3 |
E16FHF003-050 |
EnoGene |
50 tests |
EUR 127 |
Description: Available in various conjugation types. |
FITC anti human CD3 |
E16FHF003-100 |
EnoGene |
100 Tests |
EUR 190.67 |
Description: Available in various conjugation types. |
anti- Human IgA antibody |
FNab04075 |
FN Test |
100µg |
EUR 702 |
|
Description: Antibody raised against Human IgA |
anti- Human IgA antibody |
FNab04076 |
FN Test |
100µg |
EUR 702 |
|
Description: Antibody raised against Human IgA |
anti- Human IgG antibody |
FNab04077 |
FN Test |
100µg |
EUR 702 |
|
Description: Antibody raised against Human IgG |
Mouse anti Human IgE |
10-1025 |
Fitzgerald |
1 mg |
EUR 392.4 |
Description: Mouse monoclonal Mouse anti Human IgE antibody |
Mouse anti Human IgE |
10-1026 |
Fitzgerald |
1 mg |
EUR 392.4 |
Description: Mouse monoclonal Mouse anti Human IgE antibody |
Mouse anti Human IgE |
10-1027 |
Fitzgerald |
1 mg |
EUR 392.4 |
Description: Mouse monoclonal Mouse anti Human IgE antibody |
Mouse anti Human IgA |
10-7808 |
Fitzgerald |
1 mg |
EUR 403.2 |
Description: Mouse anti Human IgA antibody |
Mouse anti Human IgA |
10-I05A |
Fitzgerald |
1 mg |
EUR 369.6 |
Description: Mouse anti Human IgA antibody |
Mouse anti Human IgE |
10-I104B |
Fitzgerald |
1 mg |
EUR 403.2 |
Description: Mouse anti Human IgE antibody |
Mouse anti Human IgE |
10-I104CC |
Fitzgerald |
1 mg |
EUR 403.2 |
Description: Mouse anti Human IgE antibody |
Mouse anti Human IgE |
10-I10A |
Fitzgerald |
1 mg |
EUR 217.2 |
Description: Mouse anti Human IgE antibody |
Mouse anti Human IgE |
10-I10C |
Fitzgerald |
1 mg |
EUR 217.2 |
Description: Mouse anti Human IgE antibody |
Mouse anti Human IgE |
10-I10E |
Fitzgerald |
1 mg |
EUR 594 |
Description: Mouse anti Human IgE antibody |
Mouse anti Human IgM |
10-I20A |
Fitzgerald |
1 mg |
EUR 229.2 |
Description: Mouse anti Human IgM antibody |
Mouse anti Human IgM |
10-I20B |
Fitzgerald |
1 mg |
EUR 354 |
Description: Mouse anti Human IgM antibody |
Mouse anti Human IgM |
10-I20G |
Fitzgerald |
1 mg |
EUR 354 |
Description: Mouse anti Human IgM antibody |
Mouse anti Human IgM |
10-I20H |
Fitzgerald |
1 mg |
EUR 224.4 |
Description: Mouse anti Human IgM antibody |
Mouse anti Human IgE |
10-S9601MND1-M0 |
Fitzgerald |
1 mg |
EUR 262.8 |
Description: Mouse anti Human IgE antibody |
Mouse anti Human IgM |
10C-CR2023M2 |
Fitzgerald |
1 mg |
EUR 184.8 |
Description: Mouse anti Human IgM antibody |
Mouse anti Human IgM |
10C-CR2023M3 |
Fitzgerald |
1 mg |
EUR 184.8 |
Description: Mouse anti Human IgM antibody |
Mouse anti Human IgA |
10C-CR6043M1 |
Fitzgerald |
1 mg |
EUR 289.2 |
Description: Mouse anti Human IgA antibody |
Mouse anti Human IgE |
10C-CR6046M3 |
Fitzgerald |
1 mg |
EUR 198 |
Description: Mouse anti Human IgE antibody |
Mouse anti Human IgE |
10C-CR6046M4 |
Fitzgerald |
1 mg |
EUR 198 |
Description: Mouse anti Human IgE antibody |
Mouse anti Human IgE |
10C-CR6046M5 |
Fitzgerald |
1 mg |
EUR 198 |
Description: Mouse anti Human IgE antibody |
Mouse anti Human IgG |
10C-CR6047M1 |
Fitzgerald |
1 mg |
EUR 211.2 |
Description: Mouse anti Human IgG antibody |
Mouse anti Human IgG |
10C-CR6047M2 |
Fitzgerald |
1 mg |
EUR 211.2 |
Description: Mouse anti Human IgG antibody |
Mouse anti Human IgE |
10R-I104d |
Fitzgerald |
1 mg |
EUR 536.4 |
Description: Mouse anti Human IgE antibody |
Mouse anti Human IgE |
10R-I104e |
Fitzgerald |
1 mg |
EUR 586.8 |
Description: Mouse anti Human IgE antibody |
Mouse anti Human IgM |
10R-I130a |
Fitzgerald |
1 mg |
EUR 382.8 |
Description: Mouse anti Human IgM antibody |
Mouse anti Human IgM |
10R-I130B |
Fitzgerald |
1 mg |
EUR 360 |
Description: Mouse anti Human IgM antibody |
Sheep anti Human IgA |
20C-CR6043SP |
Fitzgerald |
1 ml |
EUR 265.2 |
Description: Sheep anti Human IgA antibody |
Sheep anti Human IgA |
20C-CR6043SP-S |
Fitzgerald |
1 ml |
EUR 265.2 |
Description: Sheep anti Human IgA antibody |
Sheep anti Human IgA |
20-1266 |
Fitzgerald |
1ml |
EUR 776.4 |
Description: Sheep polyclonal Human IgA secondary antibody |
Sheep anti Human IgG |
20-1377 |
Fitzgerald |
1ml |
EUR 5667.6 |
Description: Sheep polyclonal Human IgG secondary antibody |
Sheep anti Human IgE |
40-IG11 |
Fitzgerald |
1 ml |
EUR 159.6 |
Description: Sheep anti Human IgE antibody |
Mouse anti Human IgE |
40R-1000 |
Fitzgerald |
100 ug |
EUR 318 |
Description: Mouse anti Human IgE antibody |
Mouse anti Human IgA |
40R-1001 |
Fitzgerald |
100 ug |
EUR 318 |
Description: Mouse anti Human IgA antibody |
Mouse anti Human IgM |
41R-1586 |
Fitzgerald |
100 ug |
EUR 318 |
Description: Monoclonal Mouse anti Human IgM antibody |
Sheep anti Human IgM |
40C-CR2023S |
Fitzgerald |
1 ml |
EUR 289.2 |
Description: Sheep anti Human IgM antibody |
Mouse anti Human IgG |
40R-1003 |
Fitzgerald |
100 ug |
EUR 318 |
Description: Mouse anti Human IgG antibody |
Mouse anti Human IgE |
40R-1004 |
Fitzgerald |
100 ug |
EUR 318 |
Description: Mouse anti Human IgE antibody |
Mouse anti Human IgM |
40R-1005 |
Fitzgerald |
100 ug |
EUR 318 |
Description: Mouse anti Human IgM antibody |
Mouse anti Human IgA |
40R-1006 |
Fitzgerald |
100 ug |
EUR 318 |
Description: Mouse anti Human IgA antibody |
Mouse anti Human IgA |
40R-1011 |
Fitzgerald |
500 ug |
EUR 678 |
Description: Mouse anti Human IgA secondary antibody |
Mouse anti Human IgA |
40R-1012 |
Fitzgerald |
500 ug |
EUR 678 |
Description: Mouse anti Human IgA secondary antibody |
Mouse Anti Human IgM |
E61I00402 |
EnoGene |
100ug |
EUR 411.6 |
Mouse anti human IgA |
E61I02101 |
EnoGene |
100ug |
EUR 411.6 |
Mouse anti human IgE |
E61I02201 |
EnoGene |
100ug |
EUR 411.6 |
human anti-human CD3 mAb |
E4A09C01 |
EnoGene |
50ug |
EUR 255 |
Description: Available in various conjugation types. |
human anti-human CD4 mAb |
E4A09C02 |
EnoGene |
50ug |
EUR 255 |
Description: Available in various conjugation types. |
human anti-human CD8 mAb |
E4A09C03 |
EnoGene |
50ug |
EUR 255 |
Description: Available in various conjugation types. |
human anti-human CD9 mAb |
E4A09C04 |
EnoGene |
50ug |
EUR 255 |
Description: Available in various conjugation types. |
human anti-human C5a mAb |
E4A09D01 |
EnoGene |
50ug |
EUR 255 |
Description: Available in various conjugation types. |
human anti-human NGF mAb |
E4A09D17 |
EnoGene |
50ug |
EUR 255 |
Description: Available in various conjugation types. |
human anti-human SAA mAb |
E4A09E01 |
EnoGene |
50ug |
EUR 255 |
Description: Available in various conjugation types. |
human anti-human PCT mAb |
E4A09E02 |
EnoGene |
50ug |
EUR 255 |
Description: Available in various conjugation types. |
human anti-human CD4 mAb |
E409C02-h100 |
EnoGene |
100μL |
EUR 395 |
Description: Available in various conjugation types. |
human anti-human CD9 mAb |
E409C03-h100 |
EnoGene |
100μL |
EUR 395 |
Description: Available in various conjugation types. |
human anti-human NGF mAb |
E409C33-h100 |
EnoGene |
100μL |
EUR 395 |
Description: Available in various conjugation types. |
Mouse anti Human IgG1 |
10-I21A |
Fitzgerald |
200 ug |
EUR 264 |
Description: Mouse anti Human IgG1 antibody |
Mouse anti Human IgG2 |
10-I22A |
Fitzgerald |
200 ug |
EUR 470.4 |
Description: Mouse anti Human IgG2 antibody |
Mouse anti Human IgG3 |
10-I23A |
Fitzgerald |
200 ug |
EUR 762 |
Description: Mouse anti Human IgG3 antibody |
Mouse anti Human IgG4 |
10-I24A |
Fitzgerald |
200 ug |
EUR 470.4 |
Description: Mouse anti Human IgG4 antibody |
Mouse anti Human IgG1 |
10R-I110a |
Fitzgerald |
1 ml |
EUR 664.8 |
Description: Mouse anti Human IgG1 antibody |
Mouse anti Human IgG1 |
10R-I127a |
Fitzgerald |
1 mg |
EUR 586.8 |
Description: Mouse anti Human IgG1 antibody |
Mouse anti Human IgG2 |
10R-I128a |
Fitzgerald |
1 mg |
EUR 586.8 |
Description: Mouse anti Human IgG2 antibody |
Mouse anti Human IgG4 |
10R-I129a |
Fitzgerald |
1 mg |
EUR 573.6 |
Description: Mouse anti Human IgG4 antibody |
Rabbit anti Human IgE |
20-IR77 |
Fitzgerald |
1 ml |
EUR 230.4 |
Description: Rabbit anti Human IgE antibody |
Rabbit anti Human IgG |
40C-CB0943 |
Fitzgerald |
1 mg |
EUR 298.8 |
Description: Rabbit anti Human IgG secondary antibody |
Rabbit anti Human IgG |
40C-CB0950 |
Fitzgerald |
5 mg |
EUR 457.2 |
Description: Rabbit anti Human IgG secondary antibody |
Rabbit anti Human IgG |
40C-CB0971 |
Fitzgerald |
2 mg |
EUR 386.4 |
Description: Rabbit anti Human IgG secondary antibody |
Rabbit anti Human IgM |
40C-CB9100 |
Fitzgerald |
2 mg |
EUR 339.6 |
Description: Rabbit anti Human IgM secondary antibody |
Rabbit anti Human IgA |
40C-CB9114 |
Fitzgerald |
2 mg |
EUR 310.8 |
Description: Rabbit anti Human IgA secondary antibody |
However, cells grown in BHI broth listerial adjusted to a low pH (6.0) did not show improved resistance to SGF. Lower resistance to SGF listerial cells growing in BHI broth may be due, in part, by the presence of glucose (0.2%). This study shows that the environment presented by the growth of L. monocytogenes in turkey deli affect its ability to survive the condition was met in the digestive tract.