Effects of pH and agitation on the growth of Listeria monocytogenes Scott A in brain heart infusion broth containing combined potassium lactate and sodium diacetate during storage at 4 or 10 degrees C.

The purpose of this study was to compare the effect of pH on the growth kinetics of Listeria monocytogenes Scott A static and agitated broth stored at 4 and 10 degrees C with and without the combination of 1.85% potassium lactate (PL) and 0.13% sodium diacetate (SDA ) (3.3% of a commercial solution of 60%, PURASAL P Opti. Form 4). PH brain heart infusion broth without (control) or with 1.85% PL + 0.13% SDA adjusted to 5.5, 6.0, 6.5, and 7.5. L. monocytogenes Scott A inoculated (at 10 (2) CFU / ml) to the pH-adjusted broth, which is stored at 4 or 10 ° C with or without agitation. At pH 5.5, listeriostatic effects observed for broth containing 1.85% PL + 0.13% SDA at 4 and 10 degrees C both with and without agitation.

At pH 6.0, 1.85% PL + 0.13% SDA completely controlled growth of L. monocytogenes Scott A in broth static at 4 degrees C up to 20 days and significantly slow the growth of pathogens in broth agitated. At 10 degrees C, the growth of L. monocytogenes Scott A was significantly decreased 1.85% PL + 0.13% SDA in broth agitated and unagitated. At pH 6.5, 1.85% PL + 0.13% SDA significantly suppressed the growth of L. monocytogenes Scott A in both 4 degrees C (P <0.001) and 10 degrees C (P <0.01). At pH 7.5, 1.85% PL + 0.13% SDA had a limited effect on the growth of L. monocytogenes Scott A in broth stored at 4 and 10 degrees C. at 4 degrees C, the agitation decreased lag time and increase the rate of growth L. monocytogenes Scott A at all pH tested. Similar but less obvious trend was observed for the broth stored at 10 degrees C. These results suggest that the combination of lactic-diacetate effectively act with low pH and temperature to inhibit the growth of L. monocytogenes Scott A.

A simple HPLC method was developed and validated for the quantification of linezolid in Brain Heart Infusion (BHI) broth. Eperezolid employed as an internal standard and sample pre-treatment procedure is simple. calibration standards ranging from 0.05 to 16 mg / L is within 7.8% accuracy and reproducibility (RSD) of less than 8.3%. Recovery is about 100% at all concentrations examined. Linezolid autosampler insert stable for at least 24 hours at ambient temperature and in BHI for 72 hours at 37 degrees C. This test is quick and ideal for the analysis of large numbers of samples.

Resistance of Listeria monocytogenes F2365 cells to a greater synthetic gastric fluid following the growth in ready-to-eat deli turkey meat than in brain heart infusion broth.

Ready-to-eat (RTE) meat shop has been categorized as high-risk foods for foodborne listeriosis contraction. Several outbreaks of listeriosis has recently been associated with the consumption of RTE deli turkey meat. In this study, we examined whether the growth of Listeria monocytogenes F2365 on commercially prepared RTE deli turkey meat causes listerial cells become more resistant to inactivation by synthetic gastric fluid (SGF). listerial cells grow on the turkey until the end of the logarithmic-early stationary phase were significantly more resistant to SGF at pH 7.0, 5.0, or 3.5 of listerial cells grown in brain heart infusion (BHI) broth. the lower pH in the liquid in the package of turkey than in BHI broth (6.5 vs. 7.5).

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However, cells grown in BHI broth listerial adjusted to a low pH (6.0) did not show improved resistance to SGF. Lower resistance to SGF listerial cells growing in BHI broth may be due, in part, by the presence of glucose (0.2%). This study shows that the environment presented by the growth of L. monocytogenes in turkey deli affect its ability to survive the condition was met in the digestive tract.