Effect of Heat Shock on the Thermotolerance and Protein Composition of Yersinia enterocolitica in Brain Heart Infusion Broth and Ground Pork.

The optimum conditions required to induce heat-shock response in Yersinia enterocolitica in brain heart infusion (BHI) broth is determined. Heat-shock protein production and an increase in cell thermotolerance Yersinia heat-shocked at ground pork when exposed to higher temperatures was also examined. Heat shocking Y. enterocolitica cells at 45 ° C for 60 minutes consistently resulted in an increase in the number of casualties for further treatment 55 or 60 ° C in BHI broth compared to control non-heat-shocked. the value of D at 55 ° C was calculated as 7.7 and 2.0 minutes and at 60 ° C of 1.6 and 1.2 minutes to heat shock and control cells, respectively.

Upon inspection of the heat-shocked cells by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), two different heat-shock protein with a molecular mass of 70.5 and 58.0 kDa was observed which is not present in the control. Evaluation of heat shock and control cell survival in ground pork expresses the values ​​of 15.6 and 6.5 minutes D55 and D60 values ​​of 6.7 and 1.7 minutes, respectively. The results showed that before heat shock can induce increased resistance in Y. enterocolitica in pork ground for higher heat treatment. The survival of Yersinia enterocolitica in the meat is cooked because the phenomenon of heat-shock response could be a cause for concern regarding the microbiological food safety.

A modified brain heart infusion (MBHI) broth and protocols Immunomagnetic separation (IMS) using antibody-coated Dynabeads® G protein developed for the enrichment and separation of Bacillus cereus in samples artificially contaminated vegetables. The MBHI consisting of BHI and 0.34 g / L magnesium sulfate, 12.08 g / L sodium pyruvate, 1.82 g / L yeast extract, and polymyxin B. The number of beads Immunomagnetic (IMBs) and time-optimized immunoreaction , The capture efficiency is 58.32% with 0.4 mg IMBs when immunoreaction time is 20 minutes. The arrest of B. cereus by IMBs not disturb the flora competitive. IMS pre-enrichment is validated by four strains of B. cereus in artificially contaminated baby sprouts, bean sprouts, lettuce, and spinach on two levels (~ 0.1 and ~ 1 CFU / g). We were able to detect and isolate B. cereus in 40/40 samples contaminated vegetables at 0.1 CFU / g with IMS after 6 hours of enrichment in MBHI.

The combination of lactate and diacetate synergistically reduce cold growth in brain heart infusion broth Listeria monocytogenes lineages.

The combination of an organic acid which is often used in the ready-to-eat to control the growth of Listeria monocytogenes during refrigerated storage. The purpose of this study was to quantitatively assess the synergies between the two inhibitors of the growth of the organic acids in conditions similar to those present in cold-smoked salmon, and to assess the effect of the evolutionary lineage of their response to growth inhibitors.

Thirteen strains of L. monocytogenes, representing the lineage I and II, planted at 7 degrees C in broth at pH 6.1 and 4.65% NaCl water phase, which is equipped with a 2% potassium lactate, 0.14% sodium diacetate , or a combination of both at the same level. Our data indicate that adapting the same lineage with these inhibitors, due to differences in growth parameters was significant only among lineages is a small effect (+/- 0.16 days, P = 0.0499) in the phase lag (lambda).

BRAIN HEART INFUSION BROTH W/O DEXTROSE

B02-114-10kg 10 kg
EUR 1532.4

BRAIN HEART INFUSION BROTH W/O DEXTROSE

B02-114-2kg 2kg
EUR 380.4

BRAIN HEART INFUSION BROTH W/O DEXTROSE

B02-114-500g 500 g
EUR 146.4

HEART INFUSION BROTH

H08-101-10kg 10 kg
EUR 1351.2

HEART INFUSION BROTH

H08-101-2Kg 2 Kg
EUR 340.8

HEART INFUSION BROTH

H08-101-500g 500 g
EUR 136.8

Brain Heart Infusion

abx082020-100g 100 g
EUR 276

Brain Heart Infusion

abx082020-100l 100 µl
EUR 237.5

Brain Heart Infusion

abx082020-1ml 1 ml Ask for price

Brain Heart Infusion

abx082020-200l 200 µl Ask for price

BRAIN HEART INFUSION AGAR

B02-112-10kg 10 kg
EUR 1496.4

BRAIN HEART INFUSION AGAR

B02-112-2kg 2kg
EUR 372

BRAIN HEART INFUSION AGAR

B02-112-500g 500 g
EUR 145.2

BRAIN HEART INFUSION SOLIDS

B02-129-10kg 10 kg
EUR 1203.6

BRAIN HEART INFUSION SOLIDS

B02-129-2Kg 2 Kg
EUR 308.4

BRAIN HEART INFUSION SOLIDS

B02-129-500g 500 g
EUR 124.8

Brain Heart Infusion (BHI)Agar - EACH

MED1432 EACH
EUR 113.4

BHI HiVeg™ w/PABA (Brain Heart Infusion

MV212-500G 1 unit
EUR 45.83
Description: BHI HiVeg™ w/PABA (Brain Heart Infusion

V Infusion Broth

M329-500G 1 unit
EUR 43.27
Description: V Infusion Broth

Potato Infusion Broth

M1833-500G 1 unit
EUR 40.51
Description: Potato Infusion Broth

HEART INFUSION AGAR

H08-100-10kg 10 kg
EUR 1399.2

HEART INFUSION AGAR

H08-100-2kg 2kg
EUR 351.6

HEART INFUSION AGAR

H08-100-500g 500 g
EUR 139.2

Brain Hrt Infusion 100gm - EACH

237400 EACH
EUR 109.35

Brain Heart Infus 500gm

211065 EACH
EUR 201.78

Brain Heart Infus 500gm - EACH

211059 EACH
EUR 178.2

Potato Infusion

41600005-1 100 g
EUR 25.12

Potato Infusion

41600005-2 500 g
EUR 83.08

Potato Infusion

41600005-3 1 kg
EUR 153.37

V Infusion Agar

M328-500G 1 unit
EUR 46.08
Description: V Infusion Agar

LM Infusion Agar

M1206-500G 1 unit
EUR 42.8
Description: LM Infusion Agar

HiVeg™ Infusion

RM191V-500G 1 unit
EUR 49.58
Description: HiVeg™ Infusion

HL Infusion Powder

RM022-500G 1 unit
EUR 34.17
Description: HL Infusion Powder

HM Infusion Powder

RM192-500G 1 unit
EUR 34.52
Description: HM Infusion Powder

POTATO INFUSION AGAR

P16-120-10kg 10 kg
EUR 2798.4

POTATO INFUSION AGAR

P16-120-2kg 2kg
EUR 655.2

POTATO INFUSION AGAR

P16-120-500g 500 g
EUR 222

Potato Infusion Agar

M174-500G 1 unit
EUR 38.38
Description: Potato Infusion Agar

Standard Infusion Agar

M883-500G 1 unit
EUR 42.8
Description: Standard Infusion Agar

HiVeg# Special Infusion

RM188V-500G 1 unit
EUR 38.25
Description: HiVeg# Special Infusion

LM Infusion HiVeg Agar

MV1206-500G 1 unit
EUR 42.8
Description: LM Infusion HiVeg Agar

HiVeg™ Infusion No. 1

RM022V-500G 1 unit
EUR 35.37
Description: HiVeg™ Infusion No. 1

HiVeg™ Infusion No. 2

RM192V-500G 1 unit
EUR 35.48
Description: HiVeg™ Infusion No. 2

Potato Infusion HiVeg Agar

MV174-500G 1 unit
EUR 38.38
Description: Potato Infusion HiVeg Agar

Standard Infusion Agar, HiVeg

MV883-500G 1 unit
EUR 42.8
Description: Standard Infusion Agar, HiVeg

Hrt Infusion Agar 500gm - EACH

244400 EACH
EUR 284.85

Blood Agar Base (Infusion Agar)

M073-100G 1 unit
EUR 13.42
Description: Blood Agar Base (Infusion Agar)

Blood Agar Base (Infusion Agar)

M073-500G 1 unit
EUR 33.1
Description: Blood Agar Base (Infusion Agar)

Blood Agar Base (Infusion Agar)

GM073-100G 1 unit
EUR 12.97
Description: Blood Agar Base (Infusion Agar)

Blood Agar Base (Infusion Agar)

GM073-500G 1 unit
EUR 38.09
Description: Blood Agar Base (Infusion Agar)

BHI Agar (Special Infusion Agar)

M211-100G 1 unit
EUR 13.42
Description: BHI Agar (Special Infusion Agar)

BHI Agar (Special Infusion Agar)

M211-500G 1 unit
EUR 33.1
Description: BHI Agar (Special Infusion Agar)

VEAL INFUSION VIRAL TRANSPORT MEDIUM

7574002 1ML
EUR 59.77

Viral Transport Medium Veal Infusion

IGVTMV1000ML each
EUR 141
Description: Viral Transport Medium Veal Infusion

Viral Transport Medium Veal Infusion

IGVTMV500ML each
EUR 81
Description: Viral Transport Medium Veal Infusion

Blood Agar Base, HiVeg (Infusion Agar,

MV073-100G 1 unit
EUR 13.42
Description: Blood Agar Base, HiVeg (Infusion Agar,

Blood Agar Base, HiVeg (Infusion Agar,

MV073-500G 1 unit
EUR 33.1
Description: Blood Agar Base, HiVeg (Infusion Agar,

BHI Agar (Special Infusion Agar), Granul

GM211-500G 1 unit
EUR 38.09
Description: BHI Agar (Special Infusion Agar), Granul

Blood HiCynth Agar Base (Infusion HiCynt

MCD073-100G 1 unit
EUR 15.46
Description: Blood HiCynth Agar Base (Infusion HiCynt

Blood HiCynth Agar Base (Infusion HiCynt

MCD073-500G 1 unit
EUR 38.09
Description: Blood HiCynth Agar Base (Infusion HiCynt

VEAL INFUSION VIRAL TRANSPORT MEDIUM, 1LTR

7574002-1LTR 1LTR
EUR 111.02

BHI HiCynth™ Agar ( Special Infusion HiC

MCD211-100G 1 unit
EUR 15.46
Description: BHI HiCynth™ Agar ( Special Infusion HiC

BHI HiCynth™ Agar ( Special Infusion HiC

MCD211-500G 1 unit
EUR 38.09
Description: BHI HiCynth™ Agar ( Special Infusion HiC

VEAL INFUSION VIRAL TRANSPORT MEDIUM, 1 ML

7574002-A 1ML
EUR 14.39

SURFLO winged infusion set - 19 gauge - cream - PK50

DD050164B PK50
EUR 83.7

Brain Heart Inf 2Kg - EACH

237200 EACH
EUR 903.15

YPD Broth (YEPD Broth)

M1363-500G 1 unit
EUR 52.25
Description: YPD Broth (YEPD Broth)

YPD Broth (YEPD Broth)

GM1363-500G 1 unit
EUR 60.05
Description: YPD Broth (YEPD Broth)

YT Broth (2X YT Broth)

GM1251-500G 1 unit
EUR 50.84
Description: YT Broth (2X YT Broth)

LB BROTH - LENNOX LB BROTH

L12-101-10kg 10 kg
EUR 680.4

LB BROTH - LENNOX LB BROTH

L12-101-2kg 2kg
EUR 194.4

LB BROTH - LENNOX LB BROTH

L12-101-500g 500 g
EUR 97.2

Yeast Malt Broth (YM Broth)

M425-100G 1 unit
EUR 14.77
Description: Yeast Malt Broth (YM Broth)

Yeast Malt Broth (YM Broth)

M425-500G 1 unit
EUR 38.04
Description: Yeast Malt Broth (YM Broth)

LURIA BROTH - MILLER'S LB BROTH

L12-112-10kg 10 kg
EUR 694.8

LURIA BROTH - MILLER'S LB BROTH

L12-112-2kg 2kg
EUR 198

LURIA BROTH - MILLER'S LB BROTH

L12-112-500g 500 g
EUR 97.2

Feeley Gorman Broth (F.G. Broth)

M812-500G 1 unit
EUR 43.37
Description: Feeley Gorman Broth (F.G. Broth)

Lactose Broth (Broth Medium D)

ME1003-100G 1 unit
EUR 10.99
Description: Lactose Broth (Broth Medium D)

Lactose Broth (Broth Medium D)

ME1003-500G 1 unit
EUR 25.97
Description: Lactose Broth (Broth Medium D)

Lactose Broth (Broth Medium D)

M1003B-100G 1 unit
EUR 10.99
Description: Lactose Broth (Broth Medium D)

Lactose Broth (Broth Medium D)

M1003B-500G 1 unit
EUR 25.97
Description: Lactose Broth (Broth Medium D)

Lactose Sulphite Broth Base (Broth

M1287B-500G 1 unit
EUR 34.17
Description: Lactose Sulphite Broth Base (Broth

*Lactobacillus MRS Broth (MRS Broth)

GM369-100G 1 unit
EUR 18.33
Description: *Lactobacillus MRS Broth (MRS Broth)

*Lactobacillus MRS Broth (MRS Broth)

GM369-500G 1 unit
EUR 46.19
Description: *Lactobacillus MRS Broth (MRS Broth)

Casitose Magnesium Broth (NZM Broth)

M1249-500G 1 unit
EUR 55.11
Description: Casitose Magnesium Broth (NZM Broth)

Tartoff - Hobbs Broth (Terrific Broth)

M1250-500G 1 unit
EUR 55.87
Description: Tartoff - Hobbs Broth (Terrific Broth)

Tartoff - Hobbs Broth (Terrific Broth)

M1250G-500G 1 unit
EUR 67.44
Description: Tartoff - Hobbs Broth (Terrific Broth)

Tartoff - Hobbs Broth (Terrific Broth)

GM1250-10KG 1 unit
EUR 1260.67
Description: Tartoff - Hobbs Broth (Terrific Broth)

Tartoff - Hobbs Broth (Terrific Broth)

GM1250-500G 1 unit
EUR 64.25
Description: Tartoff - Hobbs Broth (Terrific Broth)

AATCC Bacteriostasis Broth (FDA Broth)

M221-500G 1 unit
EUR 44.15
Description: AATCC Bacteriostasis Broth (FDA Broth)

Ethyl Violet Azide Broth (E.V.A. Broth)

M426-500G 1 unit
EUR 36.99
Description: Ethyl Violet Azide Broth (E.V.A. Broth)

Ethyl Violet Azide Broth (E.V.A. Broth)

M426S-100G 1 unit
EUR 15.93
Description: Ethyl Violet Azide Broth (E.V.A. Broth)

Ethyl Violet Azide Broth (E.V.A. Broth)

M426S-500G 1 unit
EUR 42.55
Description: Ethyl Violet Azide Broth (E.V.A. Broth)

MR-VP Broth (Glucose Phosphate Broth)

LQ082-25X5ML 1 unit
EUR 10.27
Description: MR-VP Broth (Glucose Phosphate Broth)

MR-VP Broth (Glucose Phosphate Broth)

LQ082-50X5ML 1 unit
EUR 15.93
Description: MR-VP Broth (Glucose Phosphate Broth)

Selenite Broth (Selenite F Broth) (Twin

M052-100G 1 unit
EUR 17.21
Description: Selenite Broth (Selenite F Broth) (Twin

Selenite Broth (Selenite F Broth) (Twin

M052-500G 1 unit
EUR 47.43
Description: Selenite Broth (Selenite F Broth) (Twin

Selenite Broth (Selenite F Broth) (Twin

GM052-500G 1 unit
EUR 54.52
Description: Selenite Broth (Selenite F Broth) (Twin

ITC HiVeg Broth Base (TTC HiVeg Broth

MV1220-500G 1 unit
EUR 52.25
Description: ITC HiVeg Broth Base (TTC HiVeg Broth

Yeast Malt HiVeg Broth (YM HiVeg Broth)

MV425-100G 1 unit
EUR 14.77
Description: Yeast Malt HiVeg Broth (YM HiVeg Broth)

Yeast Malt HiVeg Broth (YM HiVeg Broth)

MV425-500G 1 unit
EUR 38.04
Description: Yeast Malt HiVeg Broth (YM HiVeg Broth)

cDNA - Congenital heart disease: Heart

C1236122Hd-3 40 reactions
EUR 802

cDNA - Congestive Heart Failure: Heart

C1236122Hd-6 40 reactions
EUR 989

Heart (Congenital heart disease) Lysate

XBL-10342 0.1 mg
EUR 796.2
Description: Human heart tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human heart tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the heart tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The heart tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

GN BROTH

G07-101-10kg 10 kg
EUR 1293.6

GN BROTH

G07-101-2Kg 2 Kg
EUR 327.6

GN BROTH

G07-101-500g 500 g
EUR 133.2

LB Broth

G246 500 g
EUR 105
Description: LB Broth is used for the cultivation of E. coli in molecular microbiology techniques. This medium provides nutrients for the bacteria so that it does not have to make its own, resulting in thriving colonies of E. coli.

NZ BROTH

N14-110-10kg 10 kg
EUR 1306.8

NZ BROTH

N14-110-2kg 2kg
EUR 331.2

NZ BROTH

N14-110-500g 500 g
EUR 133.2

M BROTH

M13-100-10kg 10 kg
EUR 1554

M BROTH

M13-100-2kg 2kg
EUR 385.2

M BROTH

M13-100-500g 500 g
EUR 148.8

SF BROTH

S19-109-10kg 10 kg
EUR 2197.2

SF BROTH

S19-109-2Kg 2 Kg
EUR 524.4

SF BROTH

S19-109-500g 500 g
EUR 186

YT Broth

SD7016 250g
EUR 86.1

CL Broth

M606-500G 1 unit
EUR 41.58
Description: CL Broth

For all strains, lactic lambda extended significantly, from 2.6 +/- 0.4 to 3.8 +/- 0.5 days (P <0.001), and lower the maximum growth rate (mu (max)) of 0 , 54 +/- .06 to 0.49 + / – 0.04 log (CFU / ml) / day (P <0.001), compared with control.Diacetate not effective alone, but in combination with lactic acid, a synergistic increase lambda 6.6 +/- 1.6 days (P <0.001) and a decrease in mu (max) to 0.34 +/- 0.05 log (CFU / ml) / day (P <0.001).