Effect of Heat Shock on the Thermotolerance and Protein Composition of Yersinia enterocolitica in Brain Heart Infusion Broth and Ground Pork.

The optimum conditions required to induce heat-shock response in Yersinia enterocolitica in brain heart infusion (BHI) broth is determined. Heat-shock protein production and an increase in cell thermotolerance Yersinia heat-shocked at ground pork when exposed to higher temperatures was also examined. Heat shocking Y. enterocolitica cells at 45 ° C for 60 minutes consistently resulted in an increase in the number of casualties for further treatment 55 or 60 ° C in BHI broth compared to control non-heat-shocked. the value of D at 55 ° C was calculated as 7.7 and 2.0 minutes and at 60 ° C of 1.6 and 1.2 minutes to heat shock and control cells, respectively.

Upon inspection of the heat-shocked cells by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), two different heat-shock protein with a molecular mass of 70.5 and 58.0 kDa was observed which is not present in the control. Evaluation of heat shock and control cell survival in ground pork expresses the values ​​of 15.6 and 6.5 minutes D55 and D60 values ​​of 6.7 and 1.7 minutes, respectively. The results showed that before heat shock can induce increased resistance in Y. enterocolitica in pork ground for higher heat treatment. The survival of Yersinia enterocolitica in the meat is cooked because the phenomenon of heat-shock response could be a cause for concern regarding the microbiological food safety.

A modified brain heart infusion (MBHI) broth and protocols Immunomagnetic separation (IMS) using antibody-coated Dynabeads® G protein developed for the enrichment and separation of Bacillus cereus in samples artificially contaminated vegetables. The MBHI consisting of BHI and 0.34 g / L magnesium sulfate, 12.08 g / L sodium pyruvate, 1.82 g / L yeast extract, and polymyxin B. The number of beads Immunomagnetic (IMBs) and time-optimized immunoreaction , The capture efficiency is 58.32% with 0.4 mg IMBs when immunoreaction time is 20 minutes. The arrest of B. cereus by IMBs not disturb the flora competitive. IMS pre-enrichment is validated by four strains of B. cereus in artificially contaminated baby sprouts, bean sprouts, lettuce, and spinach on two levels (~ 0.1 and ~ 1 CFU / g). We were able to detect and isolate B. cereus in 40/40 samples contaminated vegetables at 0.1 CFU / g with IMS after 6 hours of enrichment in MBHI.

The combination of lactate and diacetate synergistically reduce cold growth in brain heart infusion broth Listeria monocytogenes lineages.

The combination of an organic acid which is often used in the ready-to-eat to control the growth of Listeria monocytogenes during refrigerated storage. The purpose of this study was to quantitatively assess the synergies between the two inhibitors of the growth of the organic acids in conditions similar to those present in cold-smoked salmon, and to assess the effect of the evolutionary lineage of their response to growth inhibitors.

Thirteen strains of L. monocytogenes, representing the lineage I and II, planted at 7 degrees C in broth at pH 6.1 and 4.65% NaCl water phase, which is equipped with a 2% potassium lactate, 0.14% sodium diacetate , or a combination of both at the same level. Our data indicate that adapting the same lineage with these inhibitors, due to differences in growth parameters was significant only among lineages is a small effect (+/- 0.16 days, P = 0.0499) in the phase lag (lambda).

Brain Heart Infusion Broth (BHI Broth)
87864 100 Gms
EUR 6.84
Description: Part D

Brain Heart Infusion Broth (BHI Broth)
87864-1 500 Gms
EUR 24.8
Description: Part D

Brain Heart Infusion Broth (BHI Broth)
87864-2 5 Kg
EUR 235.2
Description: Part D

Brain Heart Infusion Broth - EACH
MED1434 EACH
EUR 124.2

Brain Heart Infusion Broth (Powder)
MBS653157-100g 100g
EUR 175

Brain Heart Infusion Broth (Powder)
MBS653157-1kg 1kg
EUR 565

Brain Heart Infusion Broth (Powder)
MBS653157-500g 500g
EUR 385

Brain Heart Infusion Broth (Powder)
MBS653157-5x1kg 5x1kg
EUR 2445

BRAIN HEART INFUSION BROTH W/O DEXTROSE
B02-114-10kg 10 kg
EUR 1532.4

BRAIN HEART INFUSION BROTH W/O DEXTROSE
B02-114-2kg 2kg
EUR 380.4

BRAIN HEART INFUSION BROTH W/O DEXTROSE
B02-114-500g 500 g
EUR 146.4

Brain Heart Infusion Broth w/o Dextrose (Powder)
MBS652861-100g 100g
EUR 340

Brain Heart Infusion Broth w/o Dextrose (Powder)
MBS652861-1kg 1kg
EUR 835

Brain Heart Infusion Broth w/o Dextrose (Powder)
MBS652861-500g 500g
EUR 570

Brain Heart Infusion Broth w/o Dextrose (Powder)
MBS652861-5x1kg 5x1kg
EUR 3665

Brain Heart Infusion Broth w/6.5% NaCl (BHI Broth w/6.5% NaCl)
25357 100 Gms
EUR 7.7
Description: Part D

Brain Heart Infusion Broth w/6.5% NaCl (BHI Broth w/6.5% NaCl)
25357-1 500 Gms
EUR 29.93
Description: Part D

HEART INFUSION BROTH
H08-101-10kg 10 kg
EUR 1351.2

HEART INFUSION BROTH
H08-101-2Kg 2 Kg
EUR 340.8

HEART INFUSION BROTH
H08-101-500g 500 g
EUR 136.8

Heart Infusion Broth (HI Broth)
75134 100 Gms
EUR 7.7
Description: Part D

Heart Infusion Broth (HI Broth)
75134-1 500 Gms
EUR 32.5
Description: Part D

Heart Infusion Broth (Powder)
MBS652800-500g 500g
EUR 435

Heart Infusion Broth (Powder)
MBS652800-5x500g 5x500g
EUR 1800

Brain Heart Infusion
abx082020-100g 100 g
EUR 276

Brain Heart Infusion
abx082020-100l 100 µl
EUR 237.5

Brain Heart Infusion
abx082020-1ml 1 ml Ask for price

Brain Heart Infusion
abx082020-200l 200 µl Ask for price

BRAIN HEART INFUSION AGAR
B02-112-10kg 10 kg
EUR 1496.4

BRAIN HEART INFUSION AGAR
B02-112-2kg 2kg
EUR 372

BRAIN HEART INFUSION AGAR
B02-112-500g 500 g
EUR 145.2

Brain Heart Infusion Agar
HY-157375 Get quote Ask for price
Description: Brain Heart Infusion Agar is growth medium for the cultivation of a wide variety of microorganisms[1].

BRAIN HEART INFUSION SOLIDS
B02-129-10kg 10 kg
EUR 1203.6

BRAIN HEART INFUSION SOLIDS
B02-129-2Kg 2 Kg
EUR 308.4

BRAIN HEART INFUSION SOLIDS
B02-129-500g 500 g
EUR 124.8

Brain-Heart Infusion Broth w/o Dextrose, NaCl (Powder)
MBS652857-100g 100g
EUR 300

Brain-Heart Infusion Broth w/o Dextrose, NaCl (Powder)
MBS652857-1kg 1kg
EUR 1280

Brain-Heart Infusion Broth w/o Dextrose, NaCl (Powder)
MBS652857-500g 500g
EUR 860

Brain-Heart Infusion Broth w/o Dextrose, NaCl (Powder)
MBS652857-5x1kg 5x1kg
EUR 5670

Bacto Brain Heart Infusion - EACH
237500 EACH
EUR 197.1

Brain Heart Infusion Agar (Powder)
MBS653248-100g 100g
EUR 245

Brain Heart Infusion Agar (Powder)
MBS653248-1kg 1kg
EUR 925

Brain Heart Infusion Agar (Powder)
MBS653248-500g 500g
EUR 570

Brain Heart Infusion Agar (Powder)
MBS653248-5x1kg 5x1kg
EUR 4070

Brain Heart Infusion Agar (BHI Agar)
94124 100 Gms
EUR 6.84
Description: Part D

Brain Heart Infusion Agar (BHI Agar)
94124-1 500 Gms
EUR 27.37
Description: Part D

Brain Heart Infusion Agar (BHI Agar)
94124-2 5 Kg
EUR 259.14
Description: Part D

Brain Heart Infusion (BHI)Agar - EACH
MED1432 EACH
EUR 113.4

BHI HiVeg™ w/PABA (Brain Heart Infusion
MV212-500G 1 unit
EUR 45.83
Description: BHI HiVeg™ w/PABA (Brain Heart Infusion

V Infusion Broth (Veal Infusion Broth)
86771 100 Gms
EUR 8.55
Description: Part D

V Infusion Broth (Veal Infusion Broth)
86771-1 500 Gms
EUR 30.79
Description: Part D

Brain Heart Infusion w/ 0.1% Agar (BHI w/ 0.1% Agar)
84560 100 Gms
EUR 8.55
Description: Part D

Brain Heart Infusion w/ 0.1% Agar (BHI w/ 0.1% Agar)
84560-1 500 Gms
EUR 30.79
Description: Part D

Brain Heart Infusion Agar, Modified (BHI Agar, Modified)
82856 100 Gms
EUR 7.7
Description: Part D

Brain Heart Infusion Agar, Modified (BHI Agar, Modified)
82856-1 500 Gms
EUR 30.79
Description: Part D

Brain Heart Infusion Agar, Modified (BHI Agar, Modified)
82856-2 5 Kg
EUR 280.53
Description: Part D

Brain Heart Infusion w/ PABA and Agar (BHI Agar w/PABA)
26671 100 Gms
EUR 9.41
Description: Part D

Brain Heart Infusion w/ PABA and Agar (BHI Agar w/PABA)
26671-1 500 Gms
EUR 37.63
Description: Part D

Brain Heart Infusion Powder (BHI Powder) BactoBio for bacteriology
64944 500 Gms
EUR 23.95
Description: Part D

Brain Heart Infusion Powder (BHI Powder) BactoBio for bacteriology
64944-1 5 Kg
EUR 224.93
Description: Part D

Liver Infusion Broth (LI Broth)
96453 100 Gms
EUR 6.84
Description: Part D

Liver Infusion Broth (LI Broth)
96453-1 500 Gms
EUR 24.8
Description: Part D

V Infusion Broth
M329-500G 1 unit
EUR 43.27
Description: V Infusion Broth

Potato Infusion Broth
M1833-500G 1 unit
EUR 40.51
Description: Potato Infusion Broth

Infusion Broth (Powder)
MBS653130-500g 500g
EUR 475

Infusion Broth (Powder)
MBS653130-5x500g 5x500g
EUR 1995

Beef Heart Infusion
HY-157374 Get quote Ask for price
Description: Beef Heart Infusion is a non-selective microbial culture medium formulation. Beef Heart Infusion provides nutrients for microbial growth. Beef Heart Infusion can be used for the culture, transplantation and rejuvenation of microorganisms.

HEART INFUSION AGAR
H08-100-10kg 10 kg
EUR 1399.2

HEART INFUSION AGAR
H08-100-2kg 2kg
EUR 351.6

HEART INFUSION AGAR
H08-100-500g 500 g
EUR 139.2

Brain-Heart Infusion Solids (Powder)
MBS653073-500g 500g
EUR 370

Brain-Heart Infusion Solids (Powder)
MBS653073-5x500g 5x500g
EUR 1510

Heart Infusion Agar (Powder)
MBS653006-500g 500g
EUR 415

Heart Infusion Agar (Powder)
MBS653006-5x500g 5x500g
EUR 1710

Heart Infusion Agar (HI Agar)
47704 100 Gms
EUR 8.55
Description: Part D

Heart Infusion Agar (HI Agar)
47704-1 500 Gms
EUR 35.92
Description: Part D

Brain Hrt Infusion 100gm - EACH
237400 EACH
EUR 109.35

Heart Infusion Powder (HI Powder) BactoBio for bacteriology
28551 500 Gms
EUR 19.67
Description: Part D

Heart Infusion Powder (HI Powder) BactoBio for bacteriology
28551-1 2.5 Kg
EUR 88.09
Description: Part D

Brain Heart Infus 500gm
211065 EACH
EUR 201.78

Brain Heart Infus 500gm - EACH
211059 EACH
EUR 178.2

V Infusion Agar (Veal Infusion Agar)
78604 100 Gms
EUR 11.12
Description: Part D

V Infusion Agar (Veal Infusion Agar)
78604-1 500 Gms
EUR 33.36
Description: Part D

Potato Infusion
41600005-1 100 g
EUR 32.34

Potato Infusion
41600005-2 500 g
EUR 106.98

Potato Infusion
41600005-3 1 kg
EUR 197.49

V Infusion Agar
M328-500G 1 unit
EUR 46.08
Description: V Infusion Agar

LM Infusion Agar
M1206-500G 1 unit
EUR 42.8
Description: LM Infusion Agar

HiVeg™ Infusion
RM191V-500G 1 unit
EUR 49.58
Description: HiVeg™ Infusion

HL Infusion Powder
RM022-500G 1 unit
EUR 34.17
Description: HL Infusion Powder

HM Infusion Powder
RM192-500G 1 unit
EUR 34.52
Description: HM Infusion Powder

Potato Infusion Agar
M174-500G 1 unit
EUR 38.38
Description: Potato Infusion Agar

POTATO INFUSION AGAR
P16-120-10kg 10 kg
EUR 2798.4

POTATO INFUSION AGAR
P16-120-2kg 2kg
EUR 655.2

POTATO INFUSION AGAR
P16-120-500g 500 g
EUR 222

Standard Infusion Agar
M883-500G 1 unit
EUR 42.8
Description: Standard Infusion Agar

HiVeg# Special Infusion
RM188V-500G 1 unit
EUR 38.25
Description: HiVeg# Special Infusion

LM Infusion HiVeg Agar
MV1206-500G 1 unit
EUR 42.8
Description: LM Infusion HiVeg Agar

HiVeg™ Infusion No. 1
RM022V-500G 1 unit
EUR 35.37
Description: HiVeg™ Infusion No. 1

HiVeg™ Infusion No. 2
RM192V-500G 1 unit
EUR 35.48
Description: HiVeg™ Infusion No. 2

Potato Infusion HiVeg Agar
MV174-500G 1 unit
EUR 38.38
Description: Potato Infusion HiVeg Agar

Standard Infusion Agar, HiVeg
MV883-500G 1 unit
EUR 42.8
Description: Standard Infusion Agar, HiVeg

Liver Infusion Agar (LI Agar)
68861 100 Gms
EUR 7.7
Description: Part D

Liver Infusion Agar (LI Agar)
68861-1 500 Gms
EUR 29.93
Description: Part D

Hrt Infusion Agar 500gm - EACH
244400 EACH
EUR 284.85

Blood Agar Base (Infusion Agar)
GM073-100G 1 unit
EUR 12.97
Description: Blood Agar Base (Infusion Agar)

Blood Agar Base (Infusion Agar)
GM073-500G 1 unit
EUR 38.09
Description: Blood Agar Base (Infusion Agar)

Blood Agar Base (Infusion Agar)
M073-100G 1 unit
EUR 13.42
Description: Blood Agar Base (Infusion Agar)

Blood Agar Base (Infusion Agar)
M073-500G 1 unit
EUR 33.1
Description: Blood Agar Base (Infusion Agar)

BHI Agar (Special Infusion Agar)
M211-100G 1 unit
EUR 13.42
Description: BHI Agar (Special Infusion Agar)

BHI Agar (Special Infusion Agar)
M211-500G 1 unit
EUR 33.1
Description: BHI Agar (Special Infusion Agar)

VEAL INFUSION VIRAL TRANSPORT MEDIUM
7574002 1ML
EUR 59.77

Viral Transport Medium Veal Infusion
MBS136574-1L 1L
EUR 265

Viral Transport Medium Veal Infusion
MBS136574-500mL 500mL
EUR 200

Viral Transport Medium Veal Infusion
MBS136574-5x1L 5x1L
EUR 1055

Viral Transport Medium Veal Infusion
IGVTMV1000ML each
EUR 141
Description: Viral Transport Medium Veal Infusion

Viral Transport Medium Veal Infusion
IGVTMV500ML each
EUR 81
Description: Viral Transport Medium Veal Infusion

Blood Agar Base, HiVeg (Infusion Agar,
MV073-100G 1 unit
EUR 13.42
Description: Blood Agar Base, HiVeg (Infusion Agar,

Blood Agar Base, HiVeg (Infusion Agar,
MV073-500G 1 unit
EUR 33.1
Description: Blood Agar Base, HiVeg (Infusion Agar,

BHI Agar (Special Infusion Agar), Granul
GM211-500G 1 unit
EUR 38.09
Description: BHI Agar (Special Infusion Agar), Granul

For all strains, lactic lambda extended significantly, from 2.6 +/- 0.4 to 3.8 +/- 0.5 days (P <0.001), and lower the maximum growth rate (mu (max)) of 0 , 54 +/- .06 to 0.49 + / – 0.04 log (CFU / ml) / day (P <0.001), compared with control.Diacetate not effective alone, but in combination with lactic acid, a synergistic increase lambda 6.6 +/- 1.6 days (P <0.001) and a decrease in mu (max) to 0.34 +/- 0.05 log (CFU / ml) / day (P <0.001).