Effect of Heat Shock on the Thermotolerance and Protein Composition of Yersinia enterocolitica in Brain Heart Infusion Broth and Ground Pork.

The optimum conditions required to induce heat-shock response in Yersinia enterocolitica in brain heart infusion (BHI) broth is determined. Heat-shock protein production and an increase in cell thermotolerance Yersinia heat-shocked at ground pork when exposed to higher temperatures was also examined. Heat shocking Y. enterocolitica cells at 45 ° C for 60 minutes consistently resulted in an increase in the number of casualties for further treatment 55 or 60 ° C in BHI broth compared to control non-heat-shocked. the value of D at 55 ° C was calculated as 7.7 and 2.0 minutes and at 60 ° C of 1.6 and 1.2 minutes to heat shock and control cells, respectively.

Upon inspection of the heat-shocked cells by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), two different heat-shock protein with a molecular mass of 70.5 and 58.0 kDa was observed which is not present in the control. Evaluation of heat shock and control cell survival in ground pork expresses the values ​​of 15.6 and 6.5 minutes D55 and D60 values ​​of 6.7 and 1.7 minutes, respectively. The results showed that before heat shock can induce increased resistance in Y. enterocolitica in pork ground for higher heat treatment. The survival of Yersinia enterocolitica in the meat is cooked because the phenomenon of heat-shock response could be a cause for concern regarding the microbiological food safety.

A modified brain heart infusion (MBHI) broth and protocols Immunomagnetic separation (IMS) using antibody-coated Dynabeads® G protein developed for the enrichment and separation of Bacillus cereus in samples artificially contaminated vegetables. The MBHI consisting of BHI and 0.34 g / L magnesium sulfate, 12.08 g / L sodium pyruvate, 1.82 g / L yeast extract, and polymyxin B. The number of beads Immunomagnetic (IMBs) and time-optimized immunoreaction , The capture efficiency is 58.32% with 0.4 mg IMBs when immunoreaction time is 20 minutes. The arrest of B. cereus by IMBs not disturb the flora competitive. IMS pre-enrichment is validated by four strains of B. cereus in artificially contaminated baby sprouts, bean sprouts, lettuce, and spinach on two levels (~ 0.1 and ~ 1 CFU / g). We were able to detect and isolate B. cereus in 40/40 samples contaminated vegetables at 0.1 CFU / g with IMS after 6 hours of enrichment in MBHI.

The combination of lactate and diacetate synergistically reduce cold growth in brain heart infusion broth Listeria monocytogenes lineages.

The combination of an organic acid which is often used in the ready-to-eat to control the growth of Listeria monocytogenes during refrigerated storage. The purpose of this study was to quantitatively assess the synergies between the two inhibitors of the growth of the organic acids in conditions similar to those present in cold-smoked salmon, and to assess the effect of the evolutionary lineage of their response to growth inhibitors.

Thirteen strains of L. monocytogenes, representing the lineage I and II, planted at 7 degrees C in broth at pH 6.1 and 4.65% NaCl water phase, which is equipped with a 2% potassium lactate, 0.14% sodium diacetate , or a combination of both at the same level. Our data indicate that adapting the same lineage with these inhibitors, due to differences in growth parameters was significant only among lineages is a small effect (+/- 0.16 days, P = 0.0499) in the phase lag (lambda).

HEART INFUSION BROTH

H08-101-10kg 10 kg
EUR 1351.2

HEART INFUSION BROTH

H08-101-2Kg 2 Kg
EUR 340.8

HEART INFUSION BROTH

H08-101-500g 500 g
EUR 136.8

BRAIN HEART INFUSION BROTH W/O DEXTROSE

B02-114-10kg 10 kg
EUR 1532.4

BRAIN HEART INFUSION BROTH W/O DEXTROSE

B02-114-2kg 2kg
EUR 380.4

BRAIN HEART INFUSION BROTH W/O DEXTROSE

B02-114-500g 500 g
EUR 146.4

Brain Heart Infusion

abx082020-100g 100 g
EUR 276

BRAIN HEART INFUSION AGAR

B02-112-10kg 10 kg
EUR 1496.4

BRAIN HEART INFUSION AGAR

B02-112-2kg 2kg
EUR 372

BRAIN HEART INFUSION AGAR

B02-112-500g 500 g
EUR 145.2

BRAIN HEART INFUSION SOLIDS

B02-129-10kg 10 kg
EUR 1203.6

BRAIN HEART INFUSION SOLIDS

B02-129-2Kg 2 Kg
EUR 308.4

BRAIN HEART INFUSION SOLIDS

B02-129-500g 500 g
EUR 124.8

Bacto Brain Heart Infusion

237500 EACH
EUR 157.32

Brain Heart Infusion (BHI)Agar

MED1432 EACH
EUR 78.66

HEART INFUSION AGAR

H08-100-10kg 10 kg
EUR 1399.2

HEART INFUSION AGAR

H08-100-2kg 2kg
EUR 351.6

HEART INFUSION AGAR

H08-100-500g 500 g
EUR 139.2

Brain Hrt Infusion 100gm

237400 EACH
EUR 82.08

Heart Inf Broth 500gm

238400 EACH
EUR 225.72

Brain Heart Infus 500gm

211059 EACH
EUR 133.38

Brain Heart Infus 500gm

211065 EACH
EUR 186.96

Brain Heart Inf 2Kg

237200 EACH
EUR 627

POTATO INFUSION AGAR

P16-120-10kg 10 kg
EUR 2798.4

POTATO INFUSION AGAR

P16-120-2kg 2kg
EUR 655.2

POTATO INFUSION AGAR

P16-120-500g 500 g
EUR 222

Hrt Infusion Agar 500gm

244400 EACH
EUR 214.32

LB BROTH - LENNOX LB BROTH

L12-101-10kg 10 kg
EUR 680.4

LB BROTH - LENNOX LB BROTH

L12-101-2kg 2kg
EUR 194.4

LB BROTH - LENNOX LB BROTH

L12-101-500g 500 g
EUR 97.2

LURIA BROTH - MILLER'S LB BROTH

L12-112-10kg 10 kg
EUR 694.8

LURIA BROTH - MILLER'S LB BROTH

L12-112-2kg 2kg
EUR 198

LURIA BROTH - MILLER'S LB BROTH

L12-112-500g 500 g
EUR 97.2

BRUCELLA BROTH

B02-120-10kg 10 kg
EUR 1340.4

BRUCELLA BROTH

B02-120-2Kg 2 Kg
EUR 338.4

BRUCELLA BROTH

B02-120-500g 500 g
EUR 135.6

BOLTON'S BROTH

B02-132-10kg 10 kg
EUR 2122.8

BOLTON'S BROTH

B02-132-2kg 2kg
EUR 508.8

BOLTON'S BROTH

B02-132-500g 500 g
EUR 182.4

Duper Broth

D7000-050 500ml
EUR 262.8

DEXTROSE BROTH

D04-108-10kg 10 kg
EUR 954

DEXTROSE BROTH

D04-108-2kg 2kg
EUR 254.4

DEXTROSE BROTH

D04-108-500g 500 g
EUR 112.8

COLUMBIA BROTH

C03-112-10kg 10 kg
EUR 1372.8

COLUMBIA BROTH

C03-112-2Kg 2 Kg
EUR 345.6

COLUMBIA BROTH

C03-112-500g 500 g
EUR 138

EUGONIC BROTH

E05-107-10kg 10 kg
EUR 1269.6

EUGONIC BROTH

E05-107-2kg 2kg
EUR 322.8

EUGONIC BROTH

E05-107-500g 500 g
EUR 132

EXTRACT BROTH

E05-109-10kg 10 kg
EUR 60

EXTRACT BROTH

E05-109-2kg 2kg
EUR 60

EXTRACT BROTH

E05-109-500g 500 g
EUR 60

M BROTH

M13-100-10kg 10 kg
EUR 1554

M BROTH

M13-100-2kg 2kg
EUR 385.2

M BROTH

M13-100-500g 500 g
EUR 148.8

MACCONKEY BROTH

M13-111-10kg 10 kg
EUR 1196.4

MACCONKEY BROTH

M13-111-2Kg 2 Kg
EUR 307.2

MACCONKEY BROTH

M13-111-500g 500 g
EUR 127.2

NUTRIENT BROTH

N14-103-10kg 10 kg
EUR 988.8

NUTRIENT BROTH

N14-103-2Kg 2 Kg
EUR 261.6

NUTRIENT BROTH

N14-103-500g 500 g
EUR 115.2

NZM BROTH

N14-104-10kg 10 kg
EUR 962.4

NZM BROTH

N14-104-2kg 2kg
EUR 256.8

NZM BROTH

N14-104-500g 500 g
EUR 112.8

NITRATE BROTH

N14-106-10kg 10 kg
EUR 1713.6

NITRATE BROTH

N14-106-2kg 2kg
EUR 420

NITRATE BROTH

N14-106-500g 500 g
EUR 157.2

NZYM BROTH

N14-109-10kg 10 kg
EUR 571.2

NZYM BROTH

N14-109-2Kg 2 Kg
EUR 171.6

NZYM BROTH

N14-109-500g 500 g
EUR 90

NZ BROTH

N14-110-10kg 10 kg
EUR 1306.8

NZ BROTH

N14-110-2kg 2kg
EUR 331.2

NZ BROTH

N14-110-500g 500 g
EUR 133.2

LACTOSE BROTH

L12-105-10kg 10 kg
EUR 931.2

LACTOSE BROTH

L12-105-2kg 2kg
EUR 249.6

LACTOSE BROTH

L12-105-500g 500 g
EUR 111.6

LIM BROTH

L12-129-10kg 10 kg
EUR 944.4

LIM BROTH

L12-129-2Kg 2 Kg
EUR 250.8

LIM BROTH

L12-129-500g 500 g
EUR 111.6

GN BROTH

G07-101-10kg 10 kg
EUR 1293.6

GN BROTH

G07-101-2Kg 2 Kg
EUR 327.6

GN BROTH

G07-101-500g 500 g
EUR 133.2

LB Broth

G246 500 g
EUR 177.6

FRASER BROTH

F06-102-10kg 10 kg
EUR 1004.4

FRASER BROTH

F06-102-2Kg 2 Kg
EUR 265.2

FRASER BROTH

F06-102-500g 500 g
EUR 116.4

PHOTOBACTERIUM BROTH

P16-143-10kg 10 kg Ask for price

PHOTOBACTERIUM BROTH

P16-143-2Kg 2 Kg Ask for price

PHOTOBACTERIUM BROTH

P16-143-500g 500 g Ask for price

HYLB Broth

SD7001 100g
EUR 75.66

SOB Broth

SD7007 250g
EUR 84.54

SOC Broth

SD7009 250g
EUR 86.1

NZCYM Broth

SD7010 100g
EUR 75.59

NZYM Broth

SD7012 100g
EUR 73.06

NZM Broth

SD7014 100g
EUR 73.06

YT Broth

SD7016 250g
EUR 86.1

2xYT Broth

SD7019 250g
EUR 86.1

YPD Broth

SD7022 250g
EUR 86.1

Super Broth

SD7026 250g
EUR 86.1

Lambda Broth

SD7029 100g
EUR 72.53

Terrific Broth

SD7035 250g
EUR 86.1

SCHAEDLER BROTH

S19-105-10kg 10 kg
EUR 1509.6

SCHAEDLER BROTH

S19-105-2Kg 2 Kg
EUR 375.6

SCHAEDLER BROTH

S19-105-500g 500 g
EUR 145.2

SELENITE BROTH

S19-107-10kg 10 kg
EUR 1018.8

SELENITE BROTH

S19-107-2kg 2kg
EUR 268.8

SELENITE BROTH

S19-107-500g 500 g
EUR 116.4

SF BROTH

S19-109-10kg 10 kg
EUR 2197.2

SF BROTH

S19-109-2Kg 2 Kg
EUR 524.4

SF BROTH

S19-109-500g 500 g
EUR 186

SUPER BROTH

S19-128-10kg 10 kg
EUR 1005.6

SUPER BROTH

S19-128-2Kg 2 Kg
EUR 265.2

SUPER BROTH

S19-128-500g 500 g
EUR 116.4

TAT BROTH

T20-100-10kg 10 kg
EUR 1393.2

TAT BROTH

T20-100-2Kg 2 Kg
EUR 349.2

TAT BROTH

T20-100-500g 500 g
EUR 139.2

TRYPTOSE BROTH

T20-116-10kg 10 kg
EUR 1082.4

TRYPTOSE BROTH

T20-116-2kg 2kg
EUR 282

TRYPTOSE BROTH

T20-116-500g 500 g
EUR 120

TPGY BROTH

T20-127-10kg 10 kg
EUR 1663.2

For all strains, lactic lambda extended significantly, from 2.6 +/- 0.4 to 3.8 +/- 0.5 days (P <0.001), and lower the maximum growth rate (mu (max)) of 0 , 54 +/- .06 to 0.49 + / – 0.04 log (CFU / ml) / day (P <0.001), compared with control.Diacetate not effective alone, but in combination with lactic acid, a synergistic increase lambda 6.6 +/- 1.6 days (P <0.001) and a decrease in mu (max) to 0.34 +/- 0.05 log (CFU / ml) / day (P <0.001).