Effect of Heat Shock on the Thermotolerance and Protein Composition of Yersinia enterocolitica in Brain Heart Infusion Broth and Ground Pork.
The optimum conditions required to induce heat-shock response in Yersinia enterocolitica in brain heart infusion (BHI) broth is determined. Heat-shock protein production and an increase in cell thermotolerance Yersinia heat-shocked at ground pork when exposed to higher temperatures was also examined. Heat shocking Y. enterocolitica cells at 45 ° C for 60 minutes consistently resulted in an increase in the number of casualties for further treatment 55 or 60 ° C in BHI broth compared to control non-heat-shocked. the value of D at 55 ° C was calculated as 7.7 and 2.0 minutes and at 60 ° C of 1.6 and 1.2 minutes to heat shock and control cells, respectively.
Upon inspection of the heat-shocked cells by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), two different heat-shock protein with a molecular mass of 70.5 and 58.0 kDa was observed which is not present in the control. Evaluation of heat shock and control cell survival in ground pork expresses the values of 15.6 and 6.5 minutes D55 and D60 values of 6.7 and 1.7 minutes, respectively. The results showed that before heat shock can induce increased resistance in Y. enterocolitica in pork ground for higher heat treatment. The survival of Yersinia enterocolitica in the meat is cooked because the phenomenon of heat-shock response could be a cause for concern regarding the microbiological food safety.
A modified brain heart infusion (MBHI) broth and protocols Immunomagnetic separation (IMS) using antibody-coated Dynabeads® G protein developed for the enrichment and separation of Bacillus cereus in samples artificially contaminated vegetables. The MBHI consisting of BHI and 0.34 g / L magnesium sulfate, 12.08 g / L sodium pyruvate, 1.82 g / L yeast extract, and polymyxin B. The number of beads Immunomagnetic (IMBs) and time-optimized immunoreaction , The capture efficiency is 58.32% with 0.4 mg IMBs when immunoreaction time is 20 minutes. The arrest of B. cereus by IMBs not disturb the flora competitive. IMS pre-enrichment is validated by four strains of B. cereus in artificially contaminated baby sprouts, bean sprouts, lettuce, and spinach on two levels (~ 0.1 and ~ 1 CFU / g). We were able to detect and isolate B. cereus in 40/40 samples contaminated vegetables at 0.1 CFU / g with IMS after 6 hours of enrichment in MBHI.
The combination of lactate and diacetate synergistically reduce cold growth in brain heart infusion broth Listeria monocytogenes lineages.
The combination of an organic acid which is often used in the ready-to-eat to control the growth of Listeria monocytogenes during refrigerated storage. The purpose of this study was to quantitatively assess the synergies between the two inhibitors of the growth of the organic acids in conditions similar to those present in cold-smoked salmon, and to assess the effect of the evolutionary lineage of their response to growth inhibitors.
Thirteen strains of L. monocytogenes, representing the lineage I and II, planted at 7 degrees C in broth at pH 6.1 and 4.65% NaCl water phase, which is equipped with a 2% potassium lactate, 0.14% sodium diacetate , or a combination of both at the same level. Our data indicate that adapting the same lineage with these inhibitors, due to differences in growth parameters was significant only among lineages is a small effect (+/- 0.16 days, P = 0.0499) in the phase lag (lambda).
BRAIN HEART INFUSION BROTH W/O DEXTROSE |
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B02-114-10kg | Alphabiosciences | 10 kg | EUR 1532.4 |
BRAIN HEART INFUSION BROTH W/O DEXTROSE |
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B02-114-2kg | Alphabiosciences | 2kg | EUR 380.4 |
BRAIN HEART INFUSION BROTH W/O DEXTROSE |
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B02-114-500g | Alphabiosciences | 500 g | EUR 146.4 |
HEART INFUSION BROTH |
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H08-101-10kg | Alphabiosciences | 10 kg | EUR 1351.2 |
HEART INFUSION BROTH |
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H08-101-2Kg | Alphabiosciences | 2 Kg | EUR 340.8 |
HEART INFUSION BROTH |
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H08-101-500g | Alphabiosciences | 500 g | EUR 136.8 |
Brain Heart Infusion |
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abx082020-100g | Abbexa | 100 g | EUR 276 |
BRAIN HEART INFUSION AGAR |
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B02-112-10kg | Alphabiosciences | 10 kg | EUR 1496.4 |
BRAIN HEART INFUSION AGAR |
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B02-112-2kg | Alphabiosciences | 2kg | EUR 372 |
BRAIN HEART INFUSION AGAR |
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B02-112-500g | Alphabiosciences | 500 g | EUR 145.2 |
Bacto Brain Heart Infusion |
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237500 | Scientific Laboratory Supplies | EACH | EUR 175.2 |
BRAIN HEART INFUSION SOLIDS |
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B02-129-10kg | Alphabiosciences | 10 kg | EUR 1203.6 |
BRAIN HEART INFUSION SOLIDS |
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B02-129-2Kg | Alphabiosciences | 2 Kg | EUR 308.4 |
BRAIN HEART INFUSION SOLIDS |
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B02-129-500g | Alphabiosciences | 500 g | EUR 124.8 |
Brain Heart Infusion (BHI)Agar |
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MED1432 | Scientific Laboratory Supplies | EACH | EUR 100.8 |
HEART INFUSION AGAR |
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H08-100-10kg | Alphabiosciences | 10 kg | EUR 1399.2 |
HEART INFUSION AGAR |
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H08-100-2kg | Alphabiosciences | 2kg | EUR 351.6 |
HEART INFUSION AGAR |
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H08-100-500g | Alphabiosciences | 500 g | EUR 139.2 |
Brain Hrt Infusion 100gm |
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237400 | Scientific Laboratory Supplies | EACH | EUR 97.2 |
Brain Heart Infus 500gm |
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211059 | Scientific Laboratory Supplies | EACH | EUR 158.4 |
Brain Heart Infus 500gm |
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211065 | Scientific Laboratory Supplies | EACH | EUR 212.4 |
Potato Infusion |
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41600005-1 | Bio-WORLD | 100 g | EUR 25.12 |
Potato Infusion |
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41600005-2 | Bio-WORLD | 500 g | EUR 83.08 |
Potato Infusion |
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41600005-3 | Bio-WORLD | 1 kg | EUR 153.37 |
POTATO INFUSION AGAR |
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P16-120-10kg | Alphabiosciences | 10 kg | EUR 2798.4 |
POTATO INFUSION AGAR |
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P16-120-2kg | Alphabiosciences | 2kg | EUR 655.2 |
POTATO INFUSION AGAR |
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P16-120-500g | Alphabiosciences | 500 g | EUR 222 |
Hrt Infusion Agar 500gm |
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244400 | Scientific Laboratory Supplies | EACH | EUR 253.2 |
Heart Inf Broth 500gm |
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238400 | Scientific Laboratory Supplies | EACH | EUR 267.6 |
Viral Transport Medium Veal Infusion |
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IGVTMV1000ML | Innovative research | each | EUR 141 |
Description: Viral Transport Medium Veal Infusion |
Viral Transport Medium Veal Infusion |
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IGVTMV500ML | Innovative research | each | EUR 81 |
Description: Viral Transport Medium Veal Infusion |
SURFLO winged infusion set - 19 gauge - cream |
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DD050164B | Scientific Laboratory Supplies | PK50 | EUR 74.4 |
Brain Heart Inf 2Kg |
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237200 | Scientific Laboratory Supplies | EACH | EUR 802.8 |
LB BROTH - LENNOX LB BROTH |
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L12-101-10kg | Alphabiosciences | 10 kg | EUR 680.4 |
LB BROTH - LENNOX LB BROTH |
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L12-101-2kg | Alphabiosciences | 2kg | EUR 194.4 |
LB BROTH - LENNOX LB BROTH |
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L12-101-500g | Alphabiosciences | 500 g | EUR 97.2 |
LURIA BROTH - MILLER'S LB BROTH |
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L12-112-10kg | Alphabiosciences | 10 kg | EUR 694.8 |
LURIA BROTH - MILLER'S LB BROTH |
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L12-112-2kg | Alphabiosciences | 2kg | EUR 198 |
LURIA BROTH - MILLER'S LB BROTH |
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L12-112-500g | Alphabiosciences | 500 g | EUR 97.2 |
NZ BROTH |
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N14-110-10kg | Alphabiosciences | 10 kg | EUR 1306.8 |
NZ BROTH |
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N14-110-2kg | Alphabiosciences | 2kg | EUR 331.2 |
NZ BROTH |
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N14-110-500g | Alphabiosciences | 500 g | EUR 133.2 |
M BROTH |
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M13-100-10kg | Alphabiosciences | 10 kg | EUR 1554 |
M BROTH |
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M13-100-2kg | Alphabiosciences | 2kg | EUR 385.2 |
M BROTH |
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M13-100-500g | Alphabiosciences | 500 g | EUR 148.8 |
B.H.I. Broth |
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MED1028 | Scientific Laboratory Supplies | PK50 | EUR 52.8 |
GN BROTH |
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G07-101-10kg | Alphabiosciences | 10 kg | EUR 1293.6 |
GN BROTH |
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G07-101-2Kg | Alphabiosciences | 2 Kg | EUR 327.6 |
GN BROTH |
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G07-101-500g | Alphabiosciences | 500 g | EUR 133.2 |
LB Broth |
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G246 | ABM | 500 g | EUR 177.6 |
YT Broth |
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SD7016 | Bio Basic | 250g | EUR 86.1 |
SF BROTH |
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S19-109-10kg | Alphabiosciences | 10 kg | EUR 2197.2 |
SF BROTH |
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S19-109-2Kg | Alphabiosciences | 2 Kg | EUR 524.4 |
SF BROTH |
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S19-109-500g | Alphabiosciences | 500 g | EUR 186 |
YM BROTH |
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Y25-108-10kg | Alphabiosciences | 10 kg | EUR 1279.2 |
YM BROTH |
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Y25-108-2kg | Alphabiosciences | 2kg | EUR 325.2 |
YM BROTH |
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Y25-108-500g | Alphabiosciences | 500 g | EUR 132 |
NZM BROTH |
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N14-104-10kg | Alphabiosciences | 10 kg | EUR 962.4 |
NZM BROTH |
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N14-104-2kg | Alphabiosciences | 2kg | EUR 256.8 |
NZM BROTH |
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N14-104-500g | Alphabiosciences | 500 g | EUR 112.8 |
LIM BROTH |
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L12-129-10kg | Alphabiosciences | 10 kg | EUR 944.4 |
LIM BROTH |
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L12-129-2Kg | Alphabiosciences | 2 Kg | EUR 250.8 |
LIM BROTH |
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L12-129-500g | Alphabiosciences | 500 g | EUR 111.6 |
MRS Broth |
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MED1636 | Scientific Laboratory Supplies | 500G | EUR 69.6 |
SOB Broth |
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SD7007 | Bio Basic | 250g | EUR 84.54 |
SOC Broth |
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SD7009 | Bio Basic | 250g | EUR 86.1 |
NZM Broth |
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SD7014 | Bio Basic | 100g | EUR 73.06 |
YPD Broth |
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SD7022 | Bio Basic | 250g | EUR 86.1 |
TAT BROTH |
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T20-100-10kg | Alphabiosciences | 10 kg | EUR 1393.2 |
TAT BROTH |
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T20-100-2Kg | Alphabiosciences | 2 Kg | EUR 349.2 |
TAT BROTH |
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T20-100-500g | Alphabiosciences | 500 g | EUR 139.2 |
YPD BROTH |
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Y25-103-10kg | Alphabiosciences | 10 kg | EUR 963.6 |
YPD BROTH |
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Y25-103-2Kg | Alphabiosciences | 2 Kg | EUR 256.8 |
YPD BROTH |
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Y25-103-500g | Alphabiosciences | 500 g | EUR 114 |
Mh Broth 500gm |
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211443 | Scientific Laboratory Supplies | EACH | EUR 205.2 |
Ym Broth 500gm |
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271120 | Scientific Laboratory Supplies | EACH | EUR 328.8 |
NZYM BROTH |
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N14-109-10kg | Alphabiosciences | 10 kg | EUR 571.2 |
NZYM BROTH |
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N14-109-2Kg | Alphabiosciences | 2 Kg | EUR 171.6 |
NZYM BROTH |
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N14-109-500g | Alphabiosciences | 500 g | EUR 90 |
Urea Broth |
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MED1076 | Scientific Laboratory Supplies | PK50 | EUR 42 |
UREA BROTH |
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U21-102B-10kg | Alphabiosciences | 10 kg | EUR 1098 |
UREA BROTH |
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U21-102B-2Kg | Alphabiosciences | 2 Kg | EUR 285.6 |
UREA BROTH |
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U21-102B-500g | Alphabiosciences | 500 g | EUR 121.2 |
HYLB Broth |
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SD7001 | Bio Basic | 100g | EUR 75.66 |
NZYM Broth |
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SD7012 | Bio Basic | 100g | EUR 73.06 |
2xYT Broth |
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SD7019 | Bio Basic | 250g | EUR 86.1 |
TPGY BROTH |
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T20-127-10kg | Alphabiosciences | 10 kg | EUR 1663.2 |
TPGY BROTH |
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T20-127-2Kg | Alphabiosciences | 2 Kg | EUR 408 |
TPGY BROTH |
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T20-127-500g | Alphabiosciences | 500 g | EUR 154.8 |
M17 Broth 500gm |
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218561 | Scientific Laboratory Supplies | EACH | EUR 306 |
Duper Broth |
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D7000-050 | GenDepot | 500ml | EUR 262.8 |
NZCYM Broth |
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SD7010 | Bio Basic | 100g | EUR 75.59 |
Super Broth |
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SD7026 | Bio Basic | 250g | EUR 86.1 |
SUPER BROTH |
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S19-128-10kg | Alphabiosciences | 10 kg | EUR 1005.6 |
SUPER BROTH |
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S19-128-2Kg | Alphabiosciences | 2 Kg | EUR 265.2 |
SUPER BROTH |
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S19-128-500g | Alphabiosciences | 500 g | EUR 116.4 |
BOLTON'S BROTH |
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B02-132-10kg | Alphabiosciences | 10 kg | EUR 2122.8 |
BOLTON'S BROTH |
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B02-132-2kg | Alphabiosciences | 2kg | EUR 508.8 |
BOLTON'S BROTH |
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B02-132-500g | Alphabiosciences | 500 g | EUR 182.4 |
FRASER BROTH |
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F06-102-10kg | Alphabiosciences | 10 kg | EUR 1004.4 |
FRASER BROTH |
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F06-102-2Kg | Alphabiosciences | 2 Kg | EUR 265.2 |
FRASER BROTH |
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F06-102-500g | Alphabiosciences | 500 g | EUR 116.4 |
Fraser Broth |
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MED1096 | Scientific Laboratory Supplies | PK50 | EUR 58.8 |
Lambda Broth |
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SD7029 | Bio Basic | 100g | EUR 72.53 |
NITRATE BROTH |
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N14-106-10kg | Alphabiosciences | 10 kg | EUR 1713.6 |
NITRATE BROTH |
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N14-106-2kg | Alphabiosciences | 2kg | EUR 420 |
NITRATE BROTH |
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N14-106-500g | Alphabiosciences | 500 g | EUR 157.2 |
EUGONIC BROTH |
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E05-107-10kg | Alphabiosciences | 10 kg | EUR 1269.6 |
EUGONIC BROTH |
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E05-107-2kg | Alphabiosciences | 2kg | EUR 322.8 |
EUGONIC BROTH |
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E05-107-500g | Alphabiosciences | 500 g | EUR 132 |
EXTRACT BROTH |
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E05-109-10kg | Alphabiosciences | 10 kg | EUR 60 |
EXTRACT BROTH |
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E05-109-2kg | Alphabiosciences | 2kg | EUR 60 |
EXTRACT BROTH |
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E05-109-500g | Alphabiosciences | 500 g | EUR 60 |
LACTOSE BROTH |
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L12-105-10kg | Alphabiosciences | 10 kg | EUR 931.2 |
LACTOSE BROTH |
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L12-105-2kg | Alphabiosciences | 2kg | EUR 249.6 |
LACTOSE BROTH |
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L12-105-500g | Alphabiosciences | 500 g | EUR 111.6 |
Lactose broth |
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MED1446 | Scientific Laboratory Supplies | EACH | EUR 88.8 |
Elliker Broth 500gm |
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212183 | Scientific Laboratory Supplies | EACH | EUR 457.2 |
Letheen Broth 500gm |
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268110 | Scientific Laboratory Supplies | EACH | EUR 375.6 |
BRUCELLA BROTH |
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B02-120-10kg | Alphabiosciences | 10 kg | EUR 1340.4 |
For all strains, lactic lambda extended significantly, from 2.6 +/- 0.4 to 3.8 +/- 0.5 days (P <0.001), and lower the maximum growth rate (mu (max)) of 0 , 54 +/- .06 to 0.49 + / – 0.04 log (CFU / ml) / day (P <0.001), compared with control.Diacetate not effective alone, but in combination with lactic acid, a synergistic increase lambda 6.6 +/- 1.6 days (P <0.001) and a decrease in mu (max) to 0.34 +/- 0.05 log (CFU / ml) / day (P <0.001).