The optimum conditions required to induce heat-shock response in Yersinia enterocolitica in brain heart infusion (BHI) broth is determined. Heat-shock protein production and an increase in cell thermotolerance Yersinia heat-shocked at ground pork when exposed to higher temperatures was also examined. Heat shocking Y. enterocolitica cells at 45 ° C for 60 minutes consistently resulted in an increase in the number of casualties for further treatment 55 or 60 ° C in BHI broth compared to control non-heat-shocked. the value of D at 55 ° C was calculated as 7.7 and 2.0 minutes and at 60 ° C of 1.6 and 1.2 minutes to heat shock and control cells, respectively.

Upon inspection of the heat-shocked cells by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), two different heat-shock protein with a molecular mass of 70.5 and 58.0 kDa was observed which is not present in the control. Evaluation of heat shock and control cell survival in ground pork expresses the values ​​of 15.6 and 6.5 minutes D55 and D60 values ​​of 6.7 and 1.7 minutes, respectively. The results showed that before heat shock can induce increased resistance in Y. enterocolitica in pork ground for higher heat treatment. The survival of Yersinia enterocolitica in the meat is cooked because the phenomenon of heat-shock response could be a cause for concern regarding the microbiological food safety.

A modified brain heart infusion (MBHI) broth and protocols Immunomagnetic separation (IMS) using antibody-coated Dynabeads® G protein developed for the enrichment and separation of Bacillus cereus in samples artificially contaminated vegetables. The MBHI consisting of BHI and 0.34 g / L magnesium sulfate, 12.08 g / L sodium pyruvate, 1.82 g / L yeast extract, and polymyxin B. The number of beads Immunomagnetic (IMBs) and time-optimized immunoreaction , The capture efficiency is 58.32% with 0.4 mg IMBs when immunoreaction time is 20 minutes. The arrest of B. cereus by IMBs not disturb the flora competitive. IMS pre-enrichment is validated by four strains of B. cereus in artificially contaminated baby sprouts, bean sprouts, lettuce, and spinach on two levels (~ 0.1 and ~ 1 CFU / g). We were able to detect and isolate B. cereus in 40/40 samples contaminated vegetables at 0.1 CFU / g with IMS after 6 hours of enrichment in MBHI.

The combination of lactate and diacetate synergistically reduce cold growth in brain heart infusion broth Listeria monocytogenes lineages.

The combination of an organic acid which is often used in the ready-to-eat to control the growth of Listeria monocytogenes during refrigerated storage. The purpose of this study was to quantitatively assess the synergies between the two inhibitors of the growth of the organic acids in conditions similar to those present in cold-smoked salmon, and to assess the effect of the evolutionary lineage of their response to growth inhibitors.

Thirteen strains of L. monocytogenes, representing the lineage I and II, planted at 7 degrees C in broth at pH 6.1 and 4.65% NaCl water phase, which is equipped with a 2% potassium lactate, 0.14% sodium diacetate , or a combination of both at the same level. Our data indicate that adapting the same lineage with these inhibitors, due to differences in growth parameters was significant only among lineages is a small effect (+/- 0.16 days, P = 0.0499) in the phase lag (lambda).

HEART INFUSION BROTH
H08-101-10kg	Alphabiosciences	10 kg	EUR 1351.2

HEART INFUSION BROTH
H08-101-2Kg	Alphabiosciences	2 Kg	EUR 340.8

HEART INFUSION BROTH
H08-101-500g	Alphabiosciences	500 g	EUR 136.8

BRAIN HEART INFUSION BROTH W/O DEXTROSE
B02-114-10kg	Alphabiosciences	10 kg	EUR 1532.4

BRAIN HEART INFUSION BROTH W/O DEXTROSE
B02-114-2kg	Alphabiosciences	2kg	EUR 380.4

BRAIN HEART INFUSION BROTH W/O DEXTROSE
B02-114-500g	Alphabiosciences	500 g	EUR 146.4

Brain Heart Infusion
abx082020-100g	Abbexa	100 g	EUR 276

BRAIN HEART INFUSION AGAR
B02-112-10kg	Alphabiosciences	10 kg	EUR 1496.4

BRAIN HEART INFUSION AGAR
B02-112-2kg	Alphabiosciences	2kg	EUR 372

BRAIN HEART INFUSION AGAR
B02-112-500g	Alphabiosciences	500 g	EUR 145.2

BRAIN HEART INFUSION SOLIDS
B02-129-10kg	Alphabiosciences	10 kg	EUR 1203.6

BRAIN HEART INFUSION SOLIDS
B02-129-2Kg	Alphabiosciences	2 Kg	EUR 308.4

BRAIN HEART INFUSION SOLIDS
B02-129-500g	Alphabiosciences	500 g	EUR 124.8

Bacto Brain Heart Infusion
237500	Scientific Laboratory Supplies	EACH	EUR 157.32

Brain Heart Infusion (BHI)Agar
MED1432	Scientific Laboratory Supplies	EACH	EUR 78.66

HEART INFUSION AGAR
H08-100-10kg	Alphabiosciences	10 kg	EUR 1399.2

HEART INFUSION AGAR
H08-100-2kg	Alphabiosciences	2kg	EUR 351.6

HEART INFUSION AGAR
H08-100-500g	Alphabiosciences	500 g	EUR 139.2

Brain Hrt Infusion 100gm
237400	Scientific Laboratory Supplies	EACH	EUR 82.08

Heart Inf Broth 500gm
238400	Scientific Laboratory Supplies	EACH	EUR 225.72

Brain Heart Infus 500gm
211059	Scientific Laboratory Supplies	EACH	EUR 133.38

Brain Heart Infus 500gm
211065	Scientific Laboratory Supplies	EACH	EUR 186.96

Brain Heart Inf 2Kg
237200	Scientific Laboratory Supplies	EACH	EUR 627

POTATO INFUSION AGAR
P16-120-10kg	Alphabiosciences	10 kg	EUR 2798.4

POTATO INFUSION AGAR
P16-120-2kg	Alphabiosciences	2kg	EUR 655.2

POTATO INFUSION AGAR
P16-120-500g	Alphabiosciences	500 g	EUR 222

Hrt Infusion Agar 500gm
244400	Scientific Laboratory Supplies	EACH	EUR 214.32

LB BROTH - LENNOX LB BROTH
L12-101-10kg	Alphabiosciences	10 kg	EUR 680.4

LB BROTH - LENNOX LB BROTH
L12-101-2kg	Alphabiosciences	2kg	EUR 194.4

LB BROTH - LENNOX LB BROTH
L12-101-500g	Alphabiosciences	500 g	EUR 97.2

LURIA BROTH - MILLER'S LB BROTH
L12-112-10kg	Alphabiosciences	10 kg	EUR 694.8

LURIA BROTH - MILLER'S LB BROTH
L12-112-2kg	Alphabiosciences	2kg	EUR 198

LURIA BROTH - MILLER'S LB BROTH
L12-112-500g	Alphabiosciences	500 g	EUR 97.2

BRUCELLA BROTH
B02-120-10kg	Alphabiosciences	10 kg	EUR 1340.4

BRUCELLA BROTH
B02-120-2Kg	Alphabiosciences	2 Kg	EUR 338.4

BRUCELLA BROTH
B02-120-500g	Alphabiosciences	500 g	EUR 135.6

BOLTON'S BROTH
B02-132-10kg	Alphabiosciences	10 kg	EUR 2122.8

BOLTON'S BROTH
B02-132-2kg	Alphabiosciences	2kg	EUR 508.8

BOLTON'S BROTH
B02-132-500g	Alphabiosciences	500 g	EUR 182.4

Duper Broth
D7000-050	GenDepot	500ml	EUR 262.8

DEXTROSE BROTH
D04-108-10kg	Alphabiosciences	10 kg	EUR 954

DEXTROSE BROTH
D04-108-2kg	Alphabiosciences	2kg	EUR 254.4

DEXTROSE BROTH
D04-108-500g	Alphabiosciences	500 g	EUR 112.8

COLUMBIA BROTH
C03-112-10kg	Alphabiosciences	10 kg	EUR 1372.8

COLUMBIA BROTH
C03-112-2Kg	Alphabiosciences	2 Kg	EUR 345.6

COLUMBIA BROTH
C03-112-500g	Alphabiosciences	500 g	EUR 138

EUGONIC BROTH
E05-107-10kg	Alphabiosciences	10 kg	EUR 1269.6

EUGONIC BROTH
E05-107-2kg	Alphabiosciences	2kg	EUR 322.8

EUGONIC BROTH
E05-107-500g	Alphabiosciences	500 g	EUR 132

EXTRACT BROTH
E05-109-10kg	Alphabiosciences	10 kg	EUR 60

EXTRACT BROTH
E05-109-2kg	Alphabiosciences	2kg	EUR 60

EXTRACT BROTH
E05-109-500g	Alphabiosciences	500 g	EUR 60

M BROTH
M13-100-10kg	Alphabiosciences	10 kg	EUR 1554

M BROTH
M13-100-2kg	Alphabiosciences	2kg	EUR 385.2

M BROTH
M13-100-500g	Alphabiosciences	500 g	EUR 148.8

MACCONKEY BROTH
M13-111-10kg	Alphabiosciences	10 kg	EUR 1196.4

MACCONKEY BROTH
M13-111-2Kg	Alphabiosciences	2 Kg	EUR 307.2

MACCONKEY BROTH
M13-111-500g	Alphabiosciences	500 g	EUR 127.2

NUTRIENT BROTH
N14-103-10kg	Alphabiosciences	10 kg	EUR 988.8

NUTRIENT BROTH
N14-103-2Kg	Alphabiosciences	2 Kg	EUR 261.6

NUTRIENT BROTH
N14-103-500g	Alphabiosciences	500 g	EUR 115.2

NZM BROTH
N14-104-10kg	Alphabiosciences	10 kg	EUR 962.4

NZM BROTH
N14-104-2kg	Alphabiosciences	2kg	EUR 256.8

NZM BROTH
N14-104-500g	Alphabiosciences	500 g	EUR 112.8

NITRATE BROTH
N14-106-10kg	Alphabiosciences	10 kg	EUR 1713.6

NITRATE BROTH
N14-106-2kg	Alphabiosciences	2kg	EUR 420

NITRATE BROTH
N14-106-500g	Alphabiosciences	500 g	EUR 157.2

NZYM BROTH
N14-109-10kg	Alphabiosciences	10 kg	EUR 571.2

NZYM BROTH
N14-109-2Kg	Alphabiosciences	2 Kg	EUR 171.6

NZYM BROTH
N14-109-500g	Alphabiosciences	500 g	EUR 90

NZ BROTH
N14-110-10kg	Alphabiosciences	10 kg	EUR 1306.8

NZ BROTH
N14-110-2kg	Alphabiosciences	2kg	EUR 331.2

NZ BROTH
N14-110-500g	Alphabiosciences	500 g	EUR 133.2

LACTOSE BROTH
L12-105-10kg	Alphabiosciences	10 kg	EUR 931.2

LACTOSE BROTH
L12-105-2kg	Alphabiosciences	2kg	EUR 249.6

LACTOSE BROTH
L12-105-500g	Alphabiosciences	500 g	EUR 111.6

LIM BROTH
L12-129-10kg	Alphabiosciences	10 kg	EUR 944.4

LIM BROTH
L12-129-2Kg	Alphabiosciences	2 Kg	EUR 250.8

LIM BROTH
L12-129-500g	Alphabiosciences	500 g	EUR 111.6

GN BROTH
G07-101-10kg	Alphabiosciences	10 kg	EUR 1293.6

GN BROTH
G07-101-2Kg	Alphabiosciences	2 Kg	EUR 327.6

GN BROTH
G07-101-500g	Alphabiosciences	500 g	EUR 133.2

LB Broth
G246	ABM	500 g	EUR 177.6

FRASER BROTH
F06-102-10kg	Alphabiosciences	10 kg	EUR 1004.4

FRASER BROTH
F06-102-2Kg	Alphabiosciences	2 Kg	EUR 265.2

FRASER BROTH
F06-102-500g	Alphabiosciences	500 g	EUR 116.4

PHOTOBACTERIUM BROTH
P16-143-10kg	Alphabiosciences	10 kg	Ask for price

PHOTOBACTERIUM BROTH
P16-143-2Kg	Alphabiosciences	2 Kg	Ask for price

PHOTOBACTERIUM BROTH
P16-143-500g	Alphabiosciences	500 g	Ask for price

HYLB Broth
SD7001	Bio Basic	100g	EUR 75.66

SOB Broth
SD7007	Bio Basic	250g	EUR 84.54

SOC Broth
SD7009	Bio Basic	250g	EUR 86.1

NZCYM Broth
SD7010	Bio Basic	100g	EUR 75.59

NZYM Broth
SD7012	Bio Basic	100g	EUR 73.06

NZM Broth
SD7014	Bio Basic	100g	EUR 73.06

YT Broth
SD7016	Bio Basic	250g	EUR 86.1

2xYT Broth
SD7019	Bio Basic	250g	EUR 86.1

YPD Broth
SD7022	Bio Basic	250g	EUR 86.1

Super Broth
SD7026	Bio Basic	250g	EUR 86.1

Lambda Broth
SD7029	Bio Basic	100g	EUR 72.53

Terrific Broth
SD7035	Bio Basic	250g	EUR 86.1

SCHAEDLER BROTH
S19-105-10kg	Alphabiosciences	10 kg	EUR 1509.6

SCHAEDLER BROTH
S19-105-2Kg	Alphabiosciences	2 Kg	EUR 375.6

SCHAEDLER BROTH
S19-105-500g	Alphabiosciences	500 g	EUR 145.2

SELENITE BROTH
S19-107-10kg	Alphabiosciences	10 kg	EUR 1018.8

SELENITE BROTH
S19-107-2kg	Alphabiosciences	2kg	EUR 268.8

SELENITE BROTH
S19-107-500g	Alphabiosciences	500 g	EUR 116.4

SF BROTH
S19-109-10kg	Alphabiosciences	10 kg	EUR 2197.2

SF BROTH
S19-109-2Kg	Alphabiosciences	2 Kg	EUR 524.4

SF BROTH
S19-109-500g	Alphabiosciences	500 g	EUR 186

SUPER BROTH
S19-128-10kg	Alphabiosciences	10 kg	EUR 1005.6

SUPER BROTH
S19-128-2Kg	Alphabiosciences	2 Kg	EUR 265.2

SUPER BROTH
S19-128-500g	Alphabiosciences	500 g	EUR 116.4

TAT BROTH
T20-100-10kg	Alphabiosciences	10 kg	EUR 1393.2

TAT BROTH
T20-100-2Kg	Alphabiosciences	2 Kg	EUR 349.2

TAT BROTH
T20-100-500g	Alphabiosciences	500 g	EUR 139.2

TRYPTOSE BROTH
T20-116-10kg	Alphabiosciences	10 kg	EUR 1082.4

TRYPTOSE BROTH
T20-116-2kg	Alphabiosciences	2kg	EUR 282

TRYPTOSE BROTH
T20-116-500g	Alphabiosciences	500 g	EUR 120

TPGY BROTH
T20-127-10kg	Alphabiosciences	10 kg	EUR 1663.2

For all strains, lactic lambda extended significantly, from 2.6 +/- 0.4 to 3.8 +/- 0.5 days (P <0.001), and lower the maximum growth rate (mu (max)) of 0 , 54 +/- .06 to 0.49 + / – 0.04 log (CFU / ml) / day (P <0.001), compared with control.Diacetate not effective alone, but in combination with lactic acid, a synergistic increase lambda 6.6 +/- 1.6 days (P <0.001) and a decrease in mu (max) to 0.34 +/- 0.05 log (CFU / ml) / day (P <0.001).