Effect of Heat Shock on the Thermotolerance and Protein Composition of Yersinia enterocolitica in Brain Heart Infusion Broth and Ground Pork.
The optimum conditions required to induce heat-shock response in Yersinia enterocolitica in brain heart infusion (BHI) broth is determined. Heat-shock protein production and an increase in cell thermotolerance Yersinia heat-shocked at ground pork when exposed to higher temperatures was also examined. Heat shocking Y. enterocolitica cells at 45 ° C for 60 minutes consistently resulted in an increase in the number of casualties for further treatment 55 or 60 ° C in BHI broth compared to control non-heat-shocked. the value of D at 55 ° C was calculated as 7.7 and 2.0 minutes and at 60 ° C of 1.6 and 1.2 minutes to heat shock and control cells, respectively.
Upon inspection of the heat-shocked cells by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), two different heat-shock protein with a molecular mass of 70.5 and 58.0 kDa was observed which is not present in the control. Evaluation of heat shock and control cell survival in ground pork expresses the values of 15.6 and 6.5 minutes D55 and D60 values of 6.7 and 1.7 minutes, respectively. The results showed that before heat shock can induce increased resistance in Y. enterocolitica in pork ground for higher heat treatment. The survival of Yersinia enterocolitica in the meat is cooked because the phenomenon of heat-shock response could be a cause for concern regarding the microbiological food safety.
A modified brain heart infusion (MBHI) broth and protocols Immunomagnetic separation (IMS) using antibody-coated Dynabeads® G protein developed for the enrichment and separation of Bacillus cereus in samples artificially contaminated vegetables. The MBHI consisting of BHI and 0.34 g / L magnesium sulfate, 12.08 g / L sodium pyruvate, 1.82 g / L yeast extract, and polymyxin B. The number of beads Immunomagnetic (IMBs) and time-optimized immunoreaction , The capture efficiency is 58.32% with 0.4 mg IMBs when immunoreaction time is 20 minutes. The arrest of B. cereus by IMBs not disturb the flora competitive. IMS pre-enrichment is validated by four strains of B. cereus in artificially contaminated baby sprouts, bean sprouts, lettuce, and spinach on two levels (~ 0.1 and ~ 1 CFU / g). We were able to detect and isolate B. cereus in 40/40 samples contaminated vegetables at 0.1 CFU / g with IMS after 6 hours of enrichment in MBHI.
The combination of lactate and diacetate synergistically reduce cold growth in brain heart infusion broth Listeria monocytogenes lineages.
The combination of an organic acid which is often used in the ready-to-eat to control the growth of Listeria monocytogenes during refrigerated storage. The purpose of this study was to quantitatively assess the synergies between the two inhibitors of the growth of the organic acids in conditions similar to those present in cold-smoked salmon, and to assess the effect of the evolutionary lineage of their response to growth inhibitors.
Thirteen strains of L. monocytogenes, representing the lineage I and II, planted at 7 degrees C in broth at pH 6.1 and 4.65% NaCl water phase, which is equipped with a 2% potassium lactate, 0.14% sodium diacetate , or a combination of both at the same level. Our data indicate that adapting the same lineage with these inhibitors, due to differences in growth parameters was significant only among lineages is a small effect (+/- 0.16 days, P = 0.0499) in the phase lag (lambda).
BRAIN HEART INFUSION BROTH W/O DEXTROSE |
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B02-114-10kg | Alphabiosciences | 10 kg | EUR 1532.4 |
BRAIN HEART INFUSION BROTH W/O DEXTROSE |
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B02-114-2kg | Alphabiosciences | 2kg | EUR 380.4 |
BRAIN HEART INFUSION BROTH W/O DEXTROSE |
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B02-114-500g | Alphabiosciences | 500 g | EUR 146.4 |
HEART INFUSION BROTH |
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H08-101-10kg | Alphabiosciences | 10 kg | EUR 1351.2 |
HEART INFUSION BROTH |
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H08-101-2Kg | Alphabiosciences | 2 Kg | EUR 340.8 |
HEART INFUSION BROTH |
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H08-101-500g | Alphabiosciences | 500 g | EUR 136.8 |
Brain Heart Infusion |
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abx082020-100g | Abbexa | 100 g | EUR 276 |
Brain Heart Infusion |
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abx082020-100l | Abbexa | 100 µl | EUR 237.5 |
Brain Heart Infusion |
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abx082020-1ml | Abbexa | 1 ml | Ask for price |
Brain Heart Infusion |
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abx082020-200l | Abbexa | 200 µl | Ask for price |
BRAIN HEART INFUSION AGAR |
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B02-112-10kg | Alphabiosciences | 10 kg | EUR 1496.4 |
BRAIN HEART INFUSION AGAR |
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B02-112-2kg | Alphabiosciences | 2kg | EUR 372 |
BRAIN HEART INFUSION AGAR |
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B02-112-500g | Alphabiosciences | 500 g | EUR 145.2 |
BRAIN HEART INFUSION SOLIDS |
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B02-129-10kg | Alphabiosciences | 10 kg | EUR 1203.6 |
BRAIN HEART INFUSION SOLIDS |
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B02-129-2Kg | Alphabiosciences | 2 Kg | EUR 308.4 |
BRAIN HEART INFUSION SOLIDS |
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B02-129-500g | Alphabiosciences | 500 g | EUR 124.8 |
Brain Heart Infusion (BHI)Agar - EACH |
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MED1432 | Scientific Laboratory Supplies | EACH | EUR 113.4 |
BHI HiVeg™ w/PABA (Brain Heart Infusion |
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MV212-500G | EWC Diagnostics | 1 unit | EUR 45.83 |
Description: BHI HiVeg™ w/PABA (Brain Heart Infusion |
V Infusion Broth |
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M329-500G | EWC Diagnostics | 1 unit | EUR 43.27 |
Description: V Infusion Broth |
Potato Infusion Broth |
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M1833-500G | EWC Diagnostics | 1 unit | EUR 40.51 |
Description: Potato Infusion Broth |
HEART INFUSION AGAR |
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H08-100-10kg | Alphabiosciences | 10 kg | EUR 1399.2 |
HEART INFUSION AGAR |
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H08-100-2kg | Alphabiosciences | 2kg | EUR 351.6 |
HEART INFUSION AGAR |
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H08-100-500g | Alphabiosciences | 500 g | EUR 139.2 |
Brain Hrt Infusion 100gm - EACH |
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237400 | Scientific Laboratory Supplies | EACH | EUR 109.35 |
Brain Heart Infus 500gm |
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211065 | Scientific Laboratory Supplies | EACH | EUR 201.78 |
Brain Heart Infus 500gm - EACH |
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211059 | Scientific Laboratory Supplies | EACH | EUR 178.2 |
Potato Infusion |
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41600005-1 | Glycomatrix | 100 g | EUR 25.12 |
Potato Infusion |
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41600005-2 | Glycomatrix | 500 g | EUR 83.08 |
Potato Infusion |
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41600005-3 | Glycomatrix | 1 kg | EUR 153.37 |
V Infusion Agar |
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M328-500G | EWC Diagnostics | 1 unit | EUR 46.08 |
Description: V Infusion Agar |
LM Infusion Agar |
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M1206-500G | EWC Diagnostics | 1 unit | EUR 42.8 |
Description: LM Infusion Agar |
HiVeg™ Infusion |
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RM191V-500G | EWC Diagnostics | 1 unit | EUR 49.58 |
Description: HiVeg™ Infusion |
HL Infusion Powder |
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RM022-500G | EWC Diagnostics | 1 unit | EUR 34.17 |
Description: HL Infusion Powder |
HM Infusion Powder |
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RM192-500G | EWC Diagnostics | 1 unit | EUR 34.52 |
Description: HM Infusion Powder |
POTATO INFUSION AGAR |
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P16-120-10kg | Alphabiosciences | 10 kg | EUR 2798.4 |
POTATO INFUSION AGAR |
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P16-120-2kg | Alphabiosciences | 2kg | EUR 655.2 |
POTATO INFUSION AGAR |
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P16-120-500g | Alphabiosciences | 500 g | EUR 222 |
Potato Infusion Agar |
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M174-500G | EWC Diagnostics | 1 unit | EUR 38.38 |
Description: Potato Infusion Agar |
Standard Infusion Agar |
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M883-500G | EWC Diagnostics | 1 unit | EUR 42.8 |
Description: Standard Infusion Agar |
HiVeg# Special Infusion |
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RM188V-500G | EWC Diagnostics | 1 unit | EUR 38.25 |
Description: HiVeg# Special Infusion |
LM Infusion HiVeg Agar |
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MV1206-500G | EWC Diagnostics | 1 unit | EUR 42.8 |
Description: LM Infusion HiVeg Agar |
HiVeg™ Infusion No. 1 |
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RM022V-500G | EWC Diagnostics | 1 unit | EUR 35.37 |
Description: HiVeg™ Infusion No. 1 |
HiVeg™ Infusion No. 2 |
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RM192V-500G | EWC Diagnostics | 1 unit | EUR 35.48 |
Description: HiVeg™ Infusion No. 2 |
Potato Infusion HiVeg Agar |
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MV174-500G | EWC Diagnostics | 1 unit | EUR 38.38 |
Description: Potato Infusion HiVeg Agar |
Standard Infusion Agar, HiVeg |
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MV883-500G | EWC Diagnostics | 1 unit | EUR 42.8 |
Description: Standard Infusion Agar, HiVeg |
Hrt Infusion Agar 500gm - EACH |
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244400 | Scientific Laboratory Supplies | EACH | EUR 284.85 |
Blood Agar Base (Infusion Agar) |
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M073-100G | EWC Diagnostics | 1 unit | EUR 13.42 |
Description: Blood Agar Base (Infusion Agar) |
Blood Agar Base (Infusion Agar) |
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M073-500G | EWC Diagnostics | 1 unit | EUR 33.1 |
Description: Blood Agar Base (Infusion Agar) |
Blood Agar Base (Infusion Agar) |
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GM073-100G | EWC Diagnostics | 1 unit | EUR 12.97 |
Description: Blood Agar Base (Infusion Agar) |
Blood Agar Base (Infusion Agar) |
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GM073-500G | EWC Diagnostics | 1 unit | EUR 38.09 |
Description: Blood Agar Base (Infusion Agar) |
BHI Agar (Special Infusion Agar) |
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M211-100G | EWC Diagnostics | 1 unit | EUR 13.42 |
Description: BHI Agar (Special Infusion Agar) |
BHI Agar (Special Infusion Agar) |
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M211-500G | EWC Diagnostics | 1 unit | EUR 33.1 |
Description: BHI Agar (Special Infusion Agar) |
VEAL INFUSION VIRAL TRANSPORT MEDIUM |
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7574002 | Lampire Biologicals Laboratories | 1ML | EUR 59.77 |
Viral Transport Medium Veal Infusion |
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IGVTMV1000ML | Innovative research | each | EUR 141 |
Description: Viral Transport Medium Veal Infusion |
Viral Transport Medium Veal Infusion |
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IGVTMV500ML | Innovative research | each | EUR 81 |
Description: Viral Transport Medium Veal Infusion |
Blood Agar Base, HiVeg (Infusion Agar, |
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MV073-100G | EWC Diagnostics | 1 unit | EUR 13.42 |
Description: Blood Agar Base, HiVeg (Infusion Agar, |
Blood Agar Base, HiVeg (Infusion Agar, |
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MV073-500G | EWC Diagnostics | 1 unit | EUR 33.1 |
Description: Blood Agar Base, HiVeg (Infusion Agar, |
BHI Agar (Special Infusion Agar), Granul |
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GM211-500G | EWC Diagnostics | 1 unit | EUR 38.09 |
Description: BHI Agar (Special Infusion Agar), Granul |
Blood HiCynth Agar Base (Infusion HiCynt |
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MCD073-100G | EWC Diagnostics | 1 unit | EUR 15.46 |
Description: Blood HiCynth Agar Base (Infusion HiCynt |
Blood HiCynth Agar Base (Infusion HiCynt |
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MCD073-500G | EWC Diagnostics | 1 unit | EUR 38.09 |
Description: Blood HiCynth Agar Base (Infusion HiCynt |
VEAL INFUSION VIRAL TRANSPORT MEDIUM, 1LTR |
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7574002-1LTR | Lampire Biologicals Laboratories | 1LTR | EUR 111.02 |
BHI HiCynth™ Agar ( Special Infusion HiC |
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MCD211-100G | EWC Diagnostics | 1 unit | EUR 15.46 |
Description: BHI HiCynth™ Agar ( Special Infusion HiC |
BHI HiCynth™ Agar ( Special Infusion HiC |
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MCD211-500G | EWC Diagnostics | 1 unit | EUR 38.09 |
Description: BHI HiCynth™ Agar ( Special Infusion HiC |
VEAL INFUSION VIRAL TRANSPORT MEDIUM, 1 ML |
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7574002-A | Lampire Biologicals Laboratories | 1ML | EUR 14.39 |
SURFLO winged infusion set - 19 gauge - cream - PK50 |
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DD050164B | Scientific Laboratory Supplies | PK50 | EUR 83.7 |
Brain Heart Inf 2Kg - EACH |
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237200 | Scientific Laboratory Supplies | EACH | EUR 903.15 |
YPD Broth (YEPD Broth) |
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M1363-500G | EWC Diagnostics | 1 unit | EUR 52.25 |
Description: YPD Broth (YEPD Broth) |
YPD Broth (YEPD Broth) |
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GM1363-500G | EWC Diagnostics | 1 unit | EUR 60.05 |
Description: YPD Broth (YEPD Broth) |
YT Broth (2X YT Broth) |
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GM1251-500G | EWC Diagnostics | 1 unit | EUR 50.84 |
Description: YT Broth (2X YT Broth) |
LB BROTH - LENNOX LB BROTH |
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L12-101-10kg | Alphabiosciences | 10 kg | EUR 680.4 |
LB BROTH - LENNOX LB BROTH |
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L12-101-2kg | Alphabiosciences | 2kg | EUR 194.4 |
LB BROTH - LENNOX LB BROTH |
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L12-101-500g | Alphabiosciences | 500 g | EUR 97.2 |
Yeast Malt Broth (YM Broth) |
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M425-100G | EWC Diagnostics | 1 unit | EUR 14.77 |
Description: Yeast Malt Broth (YM Broth) |
Yeast Malt Broth (YM Broth) |
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M425-500G | EWC Diagnostics | 1 unit | EUR 38.04 |
Description: Yeast Malt Broth (YM Broth) |
LURIA BROTH - MILLER'S LB BROTH |
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L12-112-10kg | Alphabiosciences | 10 kg | EUR 694.8 |
LURIA BROTH - MILLER'S LB BROTH |
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L12-112-2kg | Alphabiosciences | 2kg | EUR 198 |
LURIA BROTH - MILLER'S LB BROTH |
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L12-112-500g | Alphabiosciences | 500 g | EUR 97.2 |
Feeley Gorman Broth (F.G. Broth) |
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M812-500G | EWC Diagnostics | 1 unit | EUR 43.37 |
Description: Feeley Gorman Broth (F.G. Broth) |
Lactose Broth (Broth Medium D) |
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ME1003-100G | EWC Diagnostics | 1 unit | EUR 10.99 |
Description: Lactose Broth (Broth Medium D) |
Lactose Broth (Broth Medium D) |
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ME1003-500G | EWC Diagnostics | 1 unit | EUR 25.97 |
Description: Lactose Broth (Broth Medium D) |
Lactose Broth (Broth Medium D) |
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M1003B-100G | EWC Diagnostics | 1 unit | EUR 10.99 |
Description: Lactose Broth (Broth Medium D) |
Lactose Broth (Broth Medium D) |
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M1003B-500G | EWC Diagnostics | 1 unit | EUR 25.97 |
Description: Lactose Broth (Broth Medium D) |
Lactose Sulphite Broth Base (Broth |
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M1287B-500G | EWC Diagnostics | 1 unit | EUR 34.17 |
Description: Lactose Sulphite Broth Base (Broth |
*Lactobacillus MRS Broth (MRS Broth) |
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GM369-100G | EWC Diagnostics | 1 unit | EUR 18.33 |
Description: *Lactobacillus MRS Broth (MRS Broth) |
*Lactobacillus MRS Broth (MRS Broth) |
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GM369-500G | EWC Diagnostics | 1 unit | EUR 46.19 |
Description: *Lactobacillus MRS Broth (MRS Broth) |
Casitose Magnesium Broth (NZM Broth) |
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M1249-500G | EWC Diagnostics | 1 unit | EUR 55.11 |
Description: Casitose Magnesium Broth (NZM Broth) |
Tartoff - Hobbs Broth (Terrific Broth) |
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M1250-500G | EWC Diagnostics | 1 unit | EUR 55.87 |
Description: Tartoff - Hobbs Broth (Terrific Broth) |
Tartoff - Hobbs Broth (Terrific Broth) |
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M1250G-500G | EWC Diagnostics | 1 unit | EUR 67.44 |
Description: Tartoff - Hobbs Broth (Terrific Broth) |
Tartoff - Hobbs Broth (Terrific Broth) |
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GM1250-10KG | EWC Diagnostics | 1 unit | EUR 1260.67 |
Description: Tartoff - Hobbs Broth (Terrific Broth) |
Tartoff - Hobbs Broth (Terrific Broth) |
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GM1250-500G | EWC Diagnostics | 1 unit | EUR 64.25 |
Description: Tartoff - Hobbs Broth (Terrific Broth) |
AATCC Bacteriostasis Broth (FDA Broth) |
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M221-500G | EWC Diagnostics | 1 unit | EUR 44.15 |
Description: AATCC Bacteriostasis Broth (FDA Broth) |
Ethyl Violet Azide Broth (E.V.A. Broth) |
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M426-500G | EWC Diagnostics | 1 unit | EUR 36.99 |
Description: Ethyl Violet Azide Broth (E.V.A. Broth) |
Ethyl Violet Azide Broth (E.V.A. Broth) |
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M426S-100G | EWC Diagnostics | 1 unit | EUR 15.93 |
Description: Ethyl Violet Azide Broth (E.V.A. Broth) |
Ethyl Violet Azide Broth (E.V.A. Broth) |
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M426S-500G | EWC Diagnostics | 1 unit | EUR 42.55 |
Description: Ethyl Violet Azide Broth (E.V.A. Broth) |
MR-VP Broth (Glucose Phosphate Broth) |
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LQ082-25X5ML | EWC Diagnostics | 1 unit | EUR 10.27 |
Description: MR-VP Broth (Glucose Phosphate Broth) |
MR-VP Broth (Glucose Phosphate Broth) |
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LQ082-50X5ML | EWC Diagnostics | 1 unit | EUR 15.93 |
Description: MR-VP Broth (Glucose Phosphate Broth) |
Selenite Broth (Selenite F Broth) (Twin |
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M052-100G | EWC Diagnostics | 1 unit | EUR 17.21 |
Description: Selenite Broth (Selenite F Broth) (Twin |
Selenite Broth (Selenite F Broth) (Twin |
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M052-500G | EWC Diagnostics | 1 unit | EUR 47.43 |
Description: Selenite Broth (Selenite F Broth) (Twin |
Selenite Broth (Selenite F Broth) (Twin |
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GM052-500G | EWC Diagnostics | 1 unit | EUR 54.52 |
Description: Selenite Broth (Selenite F Broth) (Twin |
ITC HiVeg Broth Base (TTC HiVeg Broth |
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MV1220-500G | EWC Diagnostics | 1 unit | EUR 52.25 |
Description: ITC HiVeg Broth Base (TTC HiVeg Broth |
Yeast Malt HiVeg Broth (YM HiVeg Broth) |
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MV425-100G | EWC Diagnostics | 1 unit | EUR 14.77 |
Description: Yeast Malt HiVeg Broth (YM HiVeg Broth) |
Yeast Malt HiVeg Broth (YM HiVeg Broth) |
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MV425-500G | EWC Diagnostics | 1 unit | EUR 38.04 |
Description: Yeast Malt HiVeg Broth (YM HiVeg Broth) |
cDNA - Congenital heart disease: Heart |
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C1236122Hd-3 | Biochain | 40 reactions | EUR 802 |
cDNA - Congestive Heart Failure: Heart |
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C1236122Hd-6 | Biochain | 40 reactions | EUR 989 |
Heart (Congenital heart disease) Lysate |
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XBL-10342 | ProSci | 0.1 mg | EUR 796.2 |
Description: Human heart tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human heart tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the heart tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The heart tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
GN BROTH |
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G07-101-10kg | Alphabiosciences | 10 kg | EUR 1293.6 |
GN BROTH |
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G07-101-2Kg | Alphabiosciences | 2 Kg | EUR 327.6 |
GN BROTH |
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G07-101-500g | Alphabiosciences | 500 g | EUR 133.2 |
LB Broth |
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G246 | ABM | 500 g | EUR 105 |
Description: LB Broth is used for the cultivation of E. coli in molecular microbiology techniques. This medium provides nutrients for the bacteria so that it does not have to make its own, resulting in thriving colonies of E. coli. |
NZ BROTH |
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N14-110-10kg | Alphabiosciences | 10 kg | EUR 1306.8 |
NZ BROTH |
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N14-110-2kg | Alphabiosciences | 2kg | EUR 331.2 |
NZ BROTH |
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N14-110-500g | Alphabiosciences | 500 g | EUR 133.2 |
M BROTH |
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M13-100-10kg | Alphabiosciences | 10 kg | EUR 1554 |
M BROTH |
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M13-100-2kg | Alphabiosciences | 2kg | EUR 385.2 |
M BROTH |
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M13-100-500g | Alphabiosciences | 500 g | EUR 148.8 |
SF BROTH |
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S19-109-10kg | Alphabiosciences | 10 kg | EUR 2197.2 |
SF BROTH |
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S19-109-2Kg | Alphabiosciences | 2 Kg | EUR 524.4 |
SF BROTH |
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S19-109-500g | Alphabiosciences | 500 g | EUR 186 |
YT Broth |
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SD7016 | Bio Basic | 250g | EUR 86.1 |
CL Broth |
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M606-500G | EWC Diagnostics | 1 unit | EUR 41.58 |
Description: CL Broth |
For all strains, lactic lambda extended significantly, from 2.6 +/- 0.4 to 3.8 +/- 0.5 days (P <0.001), and lower the maximum growth rate (mu (max)) of 0 , 54 +/- .06 to 0.49 + / – 0.04 log (CFU / ml) / day (P <0.001), compared with control.Diacetate not effective alone, but in combination with lactic acid, a synergistic increase lambda 6.6 +/- 1.6 days (P <0.001) and a decrease in mu (max) to 0.34 +/- 0.05 log (CFU / ml) / day (P <0.001).