A novel brain heart infusion broth supports the study of common Francisella tularensis serotypes.

Francisella tularensis Schu S4, LVS and U112 have been a model organism for the study of the pathogenesis Francisella, and represent a cross section of different subspecies of F. tularensis. Both Schu S4 and LVS is meticulous organism, require medium enriched with supplements and nutrients for enhanced growth. Chamberlains defined medium, Tryptone Soy Broth equipped with cysteine ​​(TSBc), and cation-adjusted Mueller-Hinton broth (CAMHB) plus 2% IsoVitaleX usually used in the cultivation of these bacteria.

In this report, we describe the formulation of brain heart infusion broth simple that can be used to obtain superior growth characteristics in all these model organisms, and can promote bacterial growth from a low inoculum. Surprisingly, CAMHB, preferably in the literature for Schu S4 and LVS culture, induced the worst growth characteristics of the four formulations studied. To extend this observation, additional seven strains of F. tularensis, represent the kind of AI, A.II, and B is selected from the Department of Defense Culture Collection (UCC) and a comparative analysis of their growth characteristics carried out in four formulations broth.

Results showed differences in growth characteristics Francisella species were significantly affected by both types of strains and selection growth medium. Although four of the five additional strains of Type A is displayed superior growth characteristics in defined media Chamberlain, the growth characteristics of the three model organism, and Type B strains, which is enhanced by a new formulation based broth BHI. We conclude that this medium is an optimal choice for the cultivation of three model organisms used for research Francisella.

Brain heart infusion broth may not be the components needed for the decontamination process for the isolation of Mycobacterium avium subspecies Paratuberculosis from stool samples using ESP culture broth.

Based on laboratory experience authors suggest that the increase in bacterial contamination of Mycobacterium avium ssp. paratuberculosis (MAP) culture may be due to the addition of brain heart infusion broth (BHI) during the decontamination process, the study was designed to investigate whether BHI is a necessary component for MAP isolation from the culture broth ESP (R). Twenty-six samples of the National Veterinary Services Laboratory (NVSL) proficiency tests provided for in 2005 was used for comparison.

Two sets of paired samples are processed in the experiment. In one set, monohydrate hexadecylpyridinium chloride (HPC) and compiled antibiotics drink half strength BHI and to the other set, HPC and antibiotics drinks prepared in sterile distilled water. The culture of the 26 samples using decontamination methods BHI / HPC identified 13 (50%) is positive, while the culture using a water / HPC decontamination identified 14 (54%) were positive.

 

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The proportion of samples with positive results did not differ significantly between the methods of decontamination 2. Although in most cases take a long time to identify positive results with BHI method, the difference between the methods with respect to the number of days for a positive culture results were not statistically significant. Retrospective data collected from the Animal Disease Diagnostic Labo

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ratory, Ohio also showed that the inclusion of BHI in decontamination protocols may not have any effect on recovery MAP or the degree of contamination. Elimination of BHI broth culture can increase the sensitivity of MAP isolation, and reduce the cost of testing.