A novel brain heart infusion broth supports the study of common Francisella tularensis serotypes.

Francisella tularensis Schu S4, LVS and U112 have been a model organism for the study of the pathogenesis Francisella, and represent a cross section of different subspecies of F. tularensis. Both Schu S4 and LVS is meticulous organism, require medium enriched with supplements and nutrients for enhanced growth. Chamberlains defined medium, Tryptone Soy Broth equipped with cysteine ​​(TSBc), and cation-adjusted Mueller-Hinton broth (CAMHB) plus 2% IsoVitaleX usually used in the cultivation of these bacteria.

In this report, we describe the formulation of brain heart infusion broth simple that can be used to obtain superior growth characteristics in all these model organisms, and can promote bacterial growth from a low inoculum. Surprisingly, CAMHB, preferably in the literature for Schu S4 and LVS culture, induced the worst growth characteristics of the four formulations studied. To extend this observation, additional seven strains of F. tularensis, represent the kind of AI, A.II, and B is selected from the Department of Defense Culture Collection (UCC) and a comparative analysis of their growth characteristics carried out in four formulations broth.

Results showed differences in growth characteristics Francisella species were significantly affected by both types of strains and selection growth medium. Although four of the five additional strains of Type A is displayed superior growth characteristics in defined media Chamberlain, the growth characteristics of the three model organism, and Type B strains, which is enhanced by a new formulation based broth BHI. We conclude that this medium is an optimal choice for the cultivation of three model organisms used for research Francisella.

Brain heart infusion broth may not be the components needed for the decontamination process for the isolation of Mycobacterium avium subspecies Paratuberculosis from stool samples using ESP culture broth.

Based on laboratory experience authors suggest that the increase in bacterial contamination of Mycobacterium avium ssp. paratuberculosis (MAP) culture may be due to the addition of brain heart infusion broth (BHI) during the decontamination process, the study was designed to investigate whether BHI is a necessary component for MAP isolation from the culture broth ESP (R). Twenty-six samples of the National Veterinary Services Laboratory (NVSL) proficiency tests provided for in 2005 was used for comparison.

Two sets of paired samples are processed in the experiment. In one set, monohydrate hexadecylpyridinium chloride (HPC) and compiled antibiotics drink half strength BHI and to the other set, HPC and antibiotics drinks prepared in sterile distilled water. The culture of the 26 samples using decontamination methods BHI / HPC identified 13 (50%) is positive, while the culture using a water / HPC decontamination identified 14 (54%) were positive.

 

Mouse anti IgA1

10-I06A 1 mg
EUR 369.6
Description: Mouse anti IgA1 antibody

UltraTek Anti-Mouse

ABJ008 8 ml
EUR 96

UltraTek Anti-Mouse

ABJ015 15 ml
EUR 117.6

UltraTek Anti-Mouse

ABJ125 125 ml
EUR 273.6

UltraTek Anti-Mouse

ABJ500 500 ml
EUR 664.8

UltraTek Anti-Mouse

ABJ999 1000 ml
EUR 1120.8

SensiTek Anti-Mouse

ABC008 8 ml
EUR 85.2

SensiTek Anti-Mouse

ABC015 15 ml
EUR 96

SensiTek Anti-Mouse

ABC125 125 ml
EUR 211.2

SensiTek Anti-Mouse

ABC500 500 ml
EUR 504

SensiTek Anti-Mouse

ABC999 1000 ml
EUR 853.2

anti-mouse VLK

AR03-PA0002 100 ul
EUR 400.8
Description: Rabbit polyclonal to VLK

Mouse Anti-FcεRIα

MOFCeRIaAP(V100) 100 ug
EUR 60

Mouse Anti-FcεRIα

MOFCeRIaAP(V25) 25 ug
EUR 60

Mouse Anti-FcεRIα

MOFCeRIaB(V100) 100 ug
EUR 60

Mouse Anti-FcεRIα

MOFCeRIaB(V25) 25 ug
EUR 60

Mouse Anti-FcεRIα

MOFCeRIaCFB(V100) 100 ug
EUR 60

Mouse Anti-FcεRIα

MOFCeRIaCFB(V25) 25 ug
EUR 60

Mouse Anti-FcεRIα

MOFCeRIaF(V100) 100 ug
EUR 60

Mouse Anti-FcεRIα

MOFCeRIaF(V25) 25 ug
EUR 60

Mouse Anti-FcεRIα

MOFCeRIaF(V500) 500 ug
EUR 60

Mouse Anti-FcεRIα

MOFCeRIaPE(V100) 100 ug
EUR 60

Mouse Anti-FcεRIα

MOFCeRIaPE(V25) 25 ug
EUR 60

Mouse Anti-FcεRIα

MOFCeRIaPP(V100) 100 ug
EUR 60

Mouse Anti-FcεRIα

MOFCeRIaPP(V25) 25 ug
EUR 60

Mouse Anti-FcεRIα

MOFCeRIaPP55(V100) 100 ug
EUR 60

Mouse Anti-FcεRIα

MOFCeRIaPP55(V25) 25 ug
EUR 60

Mouse Anti-FcεRIα

MOFCeRIaPU(V100) 100 ug
EUR 60

Mouse Anti-FcεRIα

MOFCeRIaPU(V500) 500 ug
EUR 60

Mouse Anti-IL2

MOIL2A(V100) 100 ug
EUR 60

Mouse Anti-IL2

MOIL2A(V25) 25 ug
EUR 60

Mouse Anti-IL2

MOIL2B(V100) 100 ug
EUR 60

Mouse Anti-IL2

MOIL2B(V25) 25 ug
EUR 60

Mouse Anti-IL2

MOIL2CFB(V100) 100 ug
EUR 60

Mouse Anti-IL2

MOIL2CFB(V25) 25 ug
EUR 60

Mouse Anti-IL2

MOIL2F(V100) 100 ug
EUR 60

Mouse Anti-IL2

MOIL2F(V25) 25 ug
EUR 60

Mouse Anti-IL2

MOIL2F(V500) 500 ug
EUR 60

Mouse Anti-IL2

MOIL2PE(V100) 100 ug
EUR 60

Mouse Anti-IL2

MOIL2PE(V25) 25 ug
EUR 60

Mouse Anti-IL2

MOIL2PP(V100) 100 ug
EUR 60

Mouse Anti-IL2

MOIL2PP(V25) 25 ug
EUR 60

Mouse Anti-IL2

MOIL2PP5.5(V100) 100 ug
EUR 60

Mouse Anti-IL2

MOIL2PP5.5(V25) 25 ug
EUR 60

Mouse Anti-IL2

MOIL2PU(V100) 100 ug
EUR 60

Mouse Anti-IL2

MOIL2PU(V500) 500 ug
EUR 60

Mouse Anti-IL4

MOIL4A(V100) 100 ug
EUR 60

Mouse Anti-IL4

MOIL4A(V25) 25 ug
EUR 60

Mouse Anti-IL4

MOIL4B(V100) 100 ug
EUR 60

Mouse Anti-IL4

MOIL4B(V25) 25 ug
EUR 60

Mouse Anti-IL4

MOIL4CFB(V100) 100 ug
EUR 60

Mouse Anti-IL4

MOIL4CFB(V25) 25 ug
EUR 60

Mouse Anti-IL4

MOIL4F(V100) 100 ug
EUR 60

Mouse Anti-IL4

MOIL4F(V25) 25 ug
EUR 60

Mouse Anti-IL4

MOIL4F(V500) 500 ug
EUR 60

Mouse Anti-IL4

MOIL4PE(V100) 100 ug
EUR 60

Mouse Anti-IL4

MOIL4PE(V25) 25 ug
EUR 60

Mouse Anti-IL4

MOIL4PP(V100) 100 ug
EUR 60

Mouse Anti-IL4

MOIL4PP(V25) 25 ug
EUR 60

Mouse Anti-IL4

MOIL4PP5.5(V100) 100 ug
EUR 60

Mouse Anti-IL4

MOIL4PP5.5(V25) 25 ug
EUR 60

Mouse Anti-IL4

MOIL4PU(V100) 100 ug
EUR 60

Mouse Anti-IL4

MOIL4PU(V500) 500 ug
EUR 60

Mouse Anti-KLRG1

MOKLRA(V100) 100 ug
EUR 60

Mouse Anti-KLRG1

MOKLRA(V25) 25 ug
EUR 60

Mouse Anti-KLRG1

MOKLRB(V100) 100 ug
EUR 60

Mouse Anti-KLRG1

MOKLRB(V25) 25 ug
EUR 60

Mouse Anti-KLRG1

MOKLRCFB(V100) 100 ug
EUR 60

Mouse Anti-KLRG1

MOKLRCFB(V25) 25 ug
EUR 60

Mouse Anti-KLRG1

MOKLRF(V100) 100 ug
EUR 60

Mouse Anti-KLRG1

MOKLRF(V25) 25 ug
EUR 60

Mouse Anti-KLRG1

MOKLRF(V500) 500 ug
EUR 60

Mouse Anti-KLRG1

MOKLRPE(V100) 100 ug
EUR 60

Mouse Anti-KLRG1

MOKLRPE(V25) 25 ug
EUR 60

Mouse Anti-KLRG1

MOKLRPP(V100) 100 ug
EUR 60

Mouse Anti-KLRG1

MOKLRPP(V25) 25 ug
EUR 60

Mouse Anti-KLRG1

MOKLRPP5.5(V100) 100 ug
EUR 60

Mouse Anti-KLRG1

MOKLRPP5.5(V25) 25 ug
EUR 60

Mouse Anti-KLRG1

MOKLRPU(V100) 100 ug
EUR 60

Mouse Anti-KLRG1

MOKLRPU(V500) 500 ug
EUR 60

Anti-Procalcitonin antibody *Mouse anti-human, monoclonal *

V100125 50 ug
EUR 367.2

Anti-Procalcitonin antibody *Mouse anti-human, monoclonal *

V100126 1 mg
EUR 471.6

Mouse Anti Mouse Cd44 Monoclonal Antibody

CABT-46107MM 0.25 mg
EUR 795.6

Mouse anti Human IgE

10-1025 1 mg
EUR 392.4
Description: Mouse monoclonal Mouse anti Human IgE antibody

Mouse anti Human IgE

10-1026 1 mg
EUR 392.4
Description: Mouse monoclonal Mouse anti Human IgE antibody

Mouse anti Human IgE

10-1027 1 mg
EUR 392.4
Description: Mouse monoclonal Mouse anti Human IgE antibody

Mouse anti Human IgA

10-7808 1 mg
EUR 403.2
Description: Mouse anti Human IgA antibody

Mouse anti Human IgA

10-I05A 1 mg
EUR 369.6
Description: Mouse anti Human IgA antibody

Mouse anti Human IgE

10-I104B 1 mg
EUR 403.2
Description: Mouse anti Human IgE antibody

Mouse anti Human IgE

10-I104CC 1 mg
EUR 403.2
Description: Mouse anti Human IgE antibody

Mouse anti Human IgE

10-I10A 1 mg
EUR 217.2
Description: Mouse anti Human IgE antibody

Mouse anti Human IgE

10-I10C 1 mg
EUR 217.2
Description: Mouse anti Human IgE antibody

Mouse anti Human IgE

10-I10E 1 mg
EUR 594
Description: Mouse anti Human IgE antibody

Mouse anti-Canine IgE

10-I11BLY 1 mg
EUR 814.8
Description: Mouse anti-Canine IgE antibody

Mouse anti Canine IgE

10-I11D 1 mg
EUR 807.6
Description: Mouse anti Canine IgE antibody

Mouse anti IgG Fc

10-I17A 1 mg
EUR 382.8
Description: Mouse anti IgG Fc antibody

Mouse anti Human IgM

10-I20A 1 mg
EUR 229.2
Description: Mouse anti Human IgM antibody

Mouse anti Human IgM

10-I20B 1 mg
EUR 354
Description: Mouse anti Human IgM antibody

Mouse anti Human IgM

10-I20G 1 mg
EUR 354
Description: Mouse anti Human IgM antibody

Mouse anti Human IgM

10-I20H 1 mg
EUR 224.4
Description: Mouse anti Human IgM antibody

Mouse anti Human IgG1

10-I21A 200 ug
EUR 264
Description: Mouse anti Human IgG1 antibody

Mouse anti Human IgG2

10-I22A 200 ug
EUR 470.4
Description: Mouse anti Human IgG2 antibody

Mouse anti Human IgG3

10-I23A 200 ug
EUR 762
Description: Mouse anti Human IgG3 antibody

Mouse anti Human IgG4

10-I24A 200 ug
EUR 470.4
Description: Mouse anti Human IgG4 antibody

Mouse anti Human IgE

10-S9601MND1-M0 1 mg
EUR 262.8
Description: Mouse anti Human IgE antibody

Anti-CCR4 (mouse) Antibody

A00755-2 200ug
EUR 476.4
Description: Goat Polyclonal CCR4 (mouse) Antibody. Validated in ELISA, IF, IHC and tested in Mouse.

Mouse anti Rabbit IgG

40-1005 1 mg
EUR 418.8
Description: Mouse anti Rabbit IgG secondary antibody

Mouse anti Rabbit IgG

40-1006 1 mg
EUR 418.8
Description: Mouse anti Rabbit IgG secondary antibody

Goat anti Mouse IgM

41C-CB5113 2 mg
EUR 346.8
Description: Goat anti Mouse IgM secondary antibody

Rabbit anti Mouse IgG1

41C-CB5120 1 mg
EUR 373.2
Description: Rabbit anti Mouse IgG1 secondary antibody

Rabbit anti Mouse IgG2a

41C-CB5127 1 mg
EUR 373.2
Description: Rabbit anti Mouse IgG2a secondary antibody

Rabbit anti Mouse IgA

41C-CB5143 1 mg
EUR 432
Description: Rabbit anti Mouse IgA secondary antibody

Goat anti Mouse IgG

41R-1058 2 mg
EUR 178.8
Description: Goat anti Mouse IgG secondary antibody

Rat anti Mouse IgG1

41R-1114 100 ug
EUR 318
Description: Rat anti Mouse IgG1 secondary antibody

Rat anti Mouse IgG3

41R-1115 100 ug
EUR 318
Description: Rat anti Mouse IgG3 secondary antibody

Rat anti Mouse IgM

41R-1116 100 ug
EUR 318
Description: Rat anti Mouse IgM secondary antibody

Mouse anti Rat IgG1

41R-1117 100 ug
EUR 318
Description: Mouse anti Rat IgG1 secondary antibody

Mouse anti Rat IgG2a

41R-1118 100 ug
EUR 318
Description: Mouse anti Rat IgG2a secondary antibody

Mouse anti Rat IgG2b

41R-1119 100 ug
EUR 318
Description: Mouse anti Rat IgG2b secondary antibody

Mouse anti Rat IgG2c

41R-1120 100 ug
EUR 318
Description: Mouse anti Rat IgG2c secondary antibody

Mouse anti Rat IgM

41R-1122 100 ug
EUR 318
Description: Mouse anti Rat IgM secondary antibody

Mouse anti Rat IgM

41R-1123 100 ug
EUR 318
Description: Mouse anti Rat IgM secondary antibody

The proportion of samples with positive results did not differ significantly between the methods of decontamination 2. Although in most cases take a long time to identify positive results with BHI method, the difference between the methods with respect to the number of days for a positive culture results were not statistically significant. Retrospective data collected from the Animal Disease Diagnostic Labo

Clia Kits

ratory, Ohio also showed that the inclusion of BHI in decontamination protocols may not have any effect on recovery MAP or the degree of contamination. Elimination of BHI broth culture can increase the sensitivity of MAP isolation, and reduce the cost of testing.