Francisella tularensis Schu S4, LVS and U112 have been a model organism for the study of the pathogenesis Francisella, and represent a cross section of different subspecies of F. tularensis. Both Schu S4 and LVS is meticulous organism, require medium enriched with supplements and nutrients for enhanced growth. Chamberlains defined medium, Tryptone Soy Broth equipped with cysteine (TSBc), and cation-adjusted Mueller-Hinton broth (CAMHB) plus 2% IsoVitaleX usually used in the cultivation of these bacteria.
In this report, we describe the formulation of brain heart infusion broth simple that can be used to obtain superior growth characteristics in all these model organisms, and can promote bacterial growth from a low inoculum. Surprisingly, CAMHB, preferably in the literature for Schu S4 and LVS culture, induced the worst growth characteristics of the four formulations studied. To extend this observation, additional seven strains of F. tularensis, represent the kind of AI, A.II, and B is selected from the Department of Defense Culture Collection (UCC) and a comparative analysis of their growth characteristics carried out in four formulations broth.
Results showed differences in growth characteristics Francisella species were significantly affected by both types of strains and selection growth medium. Although four of the five additional strains of Type A is displayed superior growth characteristics in defined media Chamberlain, the growth characteristics of the three model organism, and Type B strains, which is enhanced by a new formulation based broth BHI. We conclude that this medium is an optimal choice for the cultivation of three model organisms used for research Francisella.
Brain heart infusion broth may not be the components needed for the decontamination process for the isolation of Mycobacterium avium subspecies Paratuberculosis from stool samples using ESP culture broth.
Based on laboratory experience authors suggest that the increase in bacterial contamination of Mycobacterium avium ssp. paratuberculosis (MAP) culture may be due to the addition of brain heart infusion broth (BHI) during the decontamination process, the study was designed to investigate whether BHI is a necessary component for MAP isolation from the culture broth ESP (R). Twenty-six samples of the National Veterinary Services Laboratory (NVSL) proficiency tests provided for in 2005 was used for comparison.
Two sets of paired samples are processed in the experiment. In one set, monohydrate hexadecylpyridinium chloride (HPC) and compiled antibiotics drink half strength BHI and to the other set, HPC and antibiotics drinks prepared in sterile distilled water. The culture of the 26 samples using decontamination methods BHI / HPC identified 13 (50%) is positive, while the culture using a water / HPC decontamination identified 14 (54%) were positive.
 Mouse Anti-IL2 |
|
MOIL2B(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-IL2 |
|
MOIL2CFB(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-IL2 |
|
MOIL2CFB(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-IL2 |
|
MOIL2F(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-IL2 |
|
MOIL2F(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-IL2 |
|
MOIL2F(V500) |
ImmunoStep |
500 ug |
EUR 60 |
Mouse Anti-IL2 |
|
MOIL2PE(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-IL2 |
|
MOIL2PE(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-IL2 |
|
MOIL2PP(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-IL2 |
|
MOIL2PP(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-IL2 |
|
MOIL2PP5.5(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-IL2 |
|
MOIL2PP5.5(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-IL2 |
|
MOIL2PU(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-IL2 |
|
MOIL2PU(V500) |
ImmunoStep |
500 ug |
EUR 60 |
 Mouse Anti-IL4 |
|
MOIL4A(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-IL4 |
|
MOIL4A(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-IL4 |
|
MOIL4B(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-IL4 |
|
MOIL4B(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-IL4 |
|
MOIL4CFB(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-IL4 |
|
MOIL4CFB(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-IL4 |
|
MOIL4F(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-IL4 |
|
MOIL4F(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-IL4 |
|
MOIL4F(V500) |
ImmunoStep |
500 ug |
EUR 60 |
Mouse Anti-IL4 |
|
MOIL4PE(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-IL4 |
|
MOIL4PE(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-IL4 |
|
MOIL4PP(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-IL4 |
|
MOIL4PP(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-IL4 |
|
MOIL4PP5.5(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-IL4 |
|
MOIL4PP5.5(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-IL4 |
|
MOIL4PU(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-IL4 |
|
MOIL4PU(V500) |
ImmunoStep |
500 ug |
EUR 60 |
 Mouse Anti-KLRG1 |
|
MOKLRA(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-KLRG1 |
|
MOKLRA(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-KLRG1 |
|
MOKLRB(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-KLRG1 |
|
MOKLRB(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-KLRG1 |
|
MOKLRCFB(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-KLRG1 |
|
MOKLRCFB(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-KLRG1 |
|
MOKLRF(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-KLRG1 |
|
MOKLRF(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-KLRG1 |
|
MOKLRF(V500) |
ImmunoStep |
500 ug |
EUR 60 |
Mouse Anti-KLRG1 |
|
MOKLRPE(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-KLRG1 |
|
MOKLRPE(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-KLRG1 |
|
MOKLRPP(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-KLRG1 |
|
MOKLRPP(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-KLRG1 |
|
MOKLRPP5.5(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-KLRG1 |
|
MOKLRPP5.5(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-KLRG1 |
|
MOKLRPU(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-KLRG1 |
|
MOKLRPU(V500) |
ImmunoStep |
500 ug |
EUR 60 |
 Mouse Anti Mouse BDNF Monoclonal Clone |
|
IMSAMLBDNFC120UL |
Innovative research |
each |
EUR 341 |
|
|
|
Description: Mouse Anti Mouse BDNF Monoclonal Clone |
 Mouse Anti-PTX Antibody, Mouse IgG2a |
|
PTX-S343 |
ACROBIOSYSTEMS |
100ug |
EUR 2043.7 |
|
|
|
Description: Mouse Anti-PTX Antibody, Mouse IgG2a is recombinantly produced from human 293 cells (HEK293). |
 PE mouse anti-mouse CD72.1 |
|
E16FMP072.1-100U |
EnoGene |
100 μg |
EUR 541.67 |
|
Description: Available in various conjugation types. |
 Mouse Anti-FcεRIα |
|
MOFCeRIaAP(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-FcεRIα |
|
MOFCeRIaAP(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-FcεRIα |
|
MOFCeRIaB(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-FcεRIα |
|
MOFCeRIaB(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-FcεRIα |
|
MOFCeRIaCFB(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-FcεRIα |
|
MOFCeRIaCFB(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-FcεRIα |
|
MOFCeRIaF(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-FcεRIα |
|
MOFCeRIaF(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-FcεRIα |
|
MOFCeRIaF(V500) |
ImmunoStep |
500 ug |
EUR 60 |
Mouse Anti-FcεRIα |
|
MOFCeRIaPE(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-FcεRIα |
|
MOFCeRIaPE(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-FcεRIα |
|
MOFCeRIaPP(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-FcεRIα |
|
MOFCeRIaPP(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-FcεRIα |
|
MOFCeRIaPP55(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-FcεRIα |
|
MOFCeRIaPP55(V25) |
ImmunoStep |
25 ug |
EUR 60 |
Mouse Anti-FcεRIα |
|
MOFCeRIaPU(V100) |
ImmunoStep |
100 ug |
EUR 60 |
Mouse Anti-FcεRIα |
|
MOFCeRIaPU(V500) |
ImmunoStep |
500 ug |
EUR 60 |
 FITC mouse anti-mouse CD72.1 |
|
E16FMF072.1-500U |
EnoGene |
500 μg |
EUR 1430 |
|
Description: Available in various conjugation types. |
 Rat anti Mouse IgE |
|
10R-I105a |
Fitzgerald |
1 mg |
EUR 650.4 |
|
Description: Rat anti Mouse IgE antibody |
 Rat anti Mouse IgG |
|
41-M61A |
Fitzgerald |
1 mg |
EUR 468 |
|
Description: Rat anti Mouse IgG antibody |
 Rat anti Mouse IgM |
|
41R-1116 |
Fitzgerald |
100 ug |
EUR 318 |
|
Description: Rat anti Mouse IgM secondary antibody |
 Biotin mouse anti-mouse CD72.1 |
|
E16FMB072.1-500U |
EnoGene |
500 μg |
EUR 1300 |
|
Description: Available in various conjugation types. |
 Goat anti Mouse IgG |
|
20-B9015G000-S0 |
Fitzgerald |
10 ml |
EUR 159.6 |
|
Description: Goat anti Mouse IgG antibody |
Goat anti Mouse IgG |
|
20-S8301G000-V0 |
Fitzgerald |
10 ml |
EUR 159.6 |
|
Description: Goat anti Mouse IgG antibody |
Goat anti Mouse IgG |
|
40-GM25S |
Fitzgerald |
100 ml |
EUR 159.6 |
|
Description: Goat anti Mouse IgG secondary antibody |
Goat anti Mouse IgG |
|
40-S8301G000-S4 |
Fitzgerald |
10 ml |
EUR 159.6 |
|
Description: Goat anti Mouse IgG secondary antibody |
Goat anti Mouse IgG |
|
41-GM25 |
Fitzgerald |
20 mg |
EUR 220.8 |
|
Description: Goat anti Mouse IgG secondary antibody |
 Goat anti Mouse IgM |
|
41C-CB5113 |
Fitzgerald |
2 mg |
EUR 346.8 |
|
Description: Goat anti Mouse IgM secondary antibody |
Goat anti Mouse IgG |
|
41R-1058 |
Fitzgerald |
2 mg |
EUR 178.8 |
|
Description: Goat anti Mouse IgG secondary antibody |
 Rat anti Mouse IgG1 |
|
41R-1114 |
Fitzgerald |
100 ug |
EUR 318 |
|
Description: Rat anti Mouse IgG1 secondary antibody |
 Rat anti Mouse IgG3 |
|
41R-1115 |
Fitzgerald |
100 ug |
EUR 318 |
|
Description: Rat anti Mouse IgG3 secondary antibody |
 Goat anti Mouse IgM |
|
FNSA-0091 |
FN Test |
500 uL |
EUR 367.92 |
|
|
|
Description: Goat anti Mouse IgM secondary antibody |
 Goat Anti Mouse IgG |
|
E61I00501 |
EnoGene |
100ug |
EUR 411.6 |
 Rat anti Mouse IgG2a |
|
10R-I117b |
Fitzgerald |
1 mg |
EUR 952.8 |
|
Description: Rat anti Mouse IgG2a antibody |
 Goat anti Mouse IgG1 |
|
20R-IG003 |
Fitzgerald |
1 mg |
EUR 262.8 |
|
Description: Goat anti Mouse IgG1 antibody |
 Goat anti Mouse IgG1 |
|
FNSA-0086 |
FN Test |
500 uL |
EUR 367.92 |
|
|
|
Description: Goat anti Mouse IgG1 secondary antibody |
 Goat anti Mouse IgG3 |
|
FNSA-0090 |
FN Test |
500 uL |
EUR 367.92 |
|
|
|
Description: Goat anti Mouse IgG3 secondary antibody |
 Rabbit anti Mouse IgM |
|
20-B80010000-U4 |
Fitzgerald |
10 ml |
EUR 230.4 |
|
Description: Rabbit anti Mouse IgM antibody |
Rabbit anti Mouse IgM |
|
40-B9201R000-R0 |
Fitzgerald |
5 ml |
EUR 159.6 |
|
Description: Rabbit anti Mouse IgM secondary antibody |
 Rabbit anti Mouse IgA |
|
41C-CB5143 |
Fitzgerald |
1 mg |
EUR 432 |
|
Description: Rabbit anti Mouse IgA secondary antibody |
 Goat anti Mouse IgG2a |
|
41R-1128 |
Fitzgerald |
1 mg |
EUR 435.6 |
|
Description: Goat anti Mouse IgG2a secondary antibody |
Rabbit anti Mouse IgM |
|
40C-CB1506 |
Fitzgerald |
2 mg |
EUR 340.8 |
|
Description: Rabbit anti Mouse IgM secondary antibody |
 Rabbit anti- mouse IgA |
|
FNab09894 |
FN Test |
100µg |
EUR 658.5 |
|
|
|
Description: Antibody raised against Rabbit mouse IgA |
 Goat anti Mouse IgG2a |
|
FNSA-0087 |
FN Test |
500 uL |
EUR 367.92 |
|
|
|
Description: Goat anti Mouse IgG2a secondary antibody |
 Goat anti Mouse IgG2b |
|
FNSA-0088 |
FN Test |
500 uL |
EUR 367.92 |
|
|
|
Description: Goat anti Mouse IgG2b secondary antibody |
 Goat anti Mouse IgG2c |
|
FNSA-0089 |
FN Test |
500 uL |
EUR 367.92 |
|
|
|
Description: Goat anti Mouse IgG2c secondary antibody |
 Rabbit anti mouse IgG |
|
E61I00901 |
EnoGene |
100ug |
EUR 411.6 |
 Rabbit anti Mouse IgG1 |
|
41C-CB5120 |
Fitzgerald |
1 mg |
EUR 373.2 |
|
Description: Rabbit anti Mouse IgG1 secondary antibody |
 Rabbit anti Mouse IgG3 |
|
40C-CB5141 |
Fitzgerald |
1 mg |
EUR 408 |
|
Description: Rabbit anti Mouse IgG3 secondary antibody |
 Rabbit anti Mouse IgG2a |
|
41C-CB5127 |
Fitzgerald |
1 mg |
EUR 373.2 |
|
Description: Rabbit anti Mouse IgG2a secondary antibody |
 Rabbit anti Mouse IgG2b |
|
40C-CB5134 |
Fitzgerald |
1 mg |
EUR 408 |
|
Description: Rabbit anti Mouse IgG2b secondary antibody |
 Mouse anti IgG Fc |
|
10-I17A |
Fitzgerald |
1 mg |
EUR 382.8 |
|
Description: Mouse anti IgG Fc antibody |
) Mouse anti IgG (Fc) |
|
40R-1010 |
Fitzgerald |
500 ug |
EUR 678 |
|
Description: Mouse anti Human IgG Fc secondary antibody |
 Mouse Anti Mouse GFAP Monoclonal Clone 3H1 |
|
IMSAMLGFAP3H1C120UL |
Innovative research |
each |
EUR 260 |
|
|
|
Description: Mouse Anti Mouse GFAP Monoclonal Clone 3H1 |
 Mouse Anti Mouse TH Monoclonal Clone 5B7D10 |
|
IMSAMLTH5B7D10C120UL |
Innovative research |
each |
EUR 282 |
|
|
|
Description: Mouse Anti Mouse TH Monoclonal Clone 5B7D10 |
 Mouse Anti Mouse tPA Monoclonal Clone H27B6 |
|
IMSAMSTPAH27B6C1MG |
Innovative research |
each |
EUR 595 |
|
|
|
Description: Mouse Anti Mouse tPA Monoclonal Clone H27B6 |
 Mouse Anti FITC Antibody |
|
E61I01401 |
EnoGene |
100ug |
EUR 411.6 |
 Mouse anti Rat IgE |
|
10R-I106a |
Fitzgerald |
1 mg |
EUR 1018.8 |
|
Description: Mouse anti Rat IgE antibody |
 Mouse anti Rat IgM |
|
41R-1122 |
Fitzgerald |
100 ug |
EUR 318 |
|
Description: Mouse anti Rat IgM secondary antibody |
Mouse anti Rat IgM |
|
41R-1123 |
Fitzgerald |
100 ug |
EUR 318 |
|
Description: Mouse anti Rat IgM secondary antibody |
 Mouse anti RBC IgG |
|
E61I01101 |
EnoGene |
10mg |
EUR 411.6 |
 Mouse Anti Mouse AIF1 Monoclonal Clone 5G4E7 |
|
IMSAMLAIF15G4E7C120UL |
Innovative research |
each |
EUR 282 |
|
|
|
Description: Mouse Anti Mouse AIF1 Monoclonal Clone 5G4E7 |
 anti- HBsAg mouse antibody |
|
HBV001 |
FN Test |
100µg |
EUR 658.5 |
|
|
|
Description: Antibody raised against HBsAg mouse |
anti- HBsAg mouse antibody |
|
HBV002 |
FN Test |
100µg |
EUR 658.5 |
|
|
|
Description: Antibody raised against HBsAg mouse |
 mouse anti-CD3 Antibody |
|
DL98566A-100ul |
DL Develop |
100 ul |
EUR 299 |
|
|
|
Description: T3D; T-cell surface glycoprotein CD3 delta chain; T-cell receptor T3 delta chain; CD antigen CD3d; T3E; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD antigen CD3e; T3G; T-cell surface glycoprotein CD3 gamma chain; T-cell receptor T3 gamma chain; CD antigen CD3g; CD3Z; T3Z; TCRZ; T-cell surface glycoprotein CD3 zeta chain; T-cell receptor T3 zeta chain; CD antigen CD247 |
The proportion of samples with positive results did not differ significantly between the methods of decontamination 2. Although in most cases take a long time to identify positive results with BHI method, the difference between the methods with respect to the number of days for a positive culture results were not statistically significant. Retrospective data collected from the Animal Disease Diagnostic Labo
ratory, Ohio also showed that the inclusion of BHI in decontamination protocols may not have any effect on recovery MAP or the degree of contamination. Elimination of BHI broth culture can increase the sensitivity of MAP isolation, and reduce the cost of testing.
