Clinical comparison of the recoveries of bloodstream pathogens in Septi-Chek brain heart infusion broth with saponin, Septi-Chek tryptic soy broth, and the isolator lysis-centrifugation system.

The recovery of microorganisms in the Septi-Chek biphasic broth tryptic soy (TSB) and Isolator blood culture system as compared to a recovery in Septi-Chek brain biphasic infusion of heart broth is equipped with an agent saponin lytic (L-BHIB; Roche Diagnostics, a Division of Hoffmann-La Roche Inc.). A total of 5,096 TSB-L-BHIB and 1,778 sets of blood culture-L-BHIB Isolator set is evaluated. There are 435 pathogenic organisms recovered in 413 (8.1%) of culture TSB-L-BHIB, with 348 organisms in TSB and 370 bottles in the bottle L-BHIB (P less than 0.05).

Significantly more isolates of yeast found in the system L-BHIB (P less than 0.01). subculture terminal from L-BHIB system is required to detect 13 clinically significant organisms. Significantly more organisms found in a bottle of L-BHIB compared with Isolator system (P less than 0.001), including Torulopsis glabrata, coagulase-negative staphylococci, and Pseudomonas species. The time required to detect all major groups of organisms are the same in both comparisons.

However, the detection of microbial growth in L-BHIB bottle macroscopic examination required sliding unit to be attached and the use of terminal subcultures after 7 days of incubation. The main advantage of L-BHIB biphasic system when compared to either TSB biphasic system or upgraded Isolator system recovery mushrooms.

Nocardia asteroides GUH-2 is not acid-fast when grown in brain heart infusion (BHI) broth. When grown in BHI broth equipped with paraffin, many filamentous cells showed the acid-fastness after treatment with 1% acid-alcohol as a decolorizing agent. When treated with 3% acid-alcohol, the cells not acid-fast filaments. In addition to acid-fast cell filaments of nocardiae, unknown acid-fast round body was observed in the culture broth BHI paraffin-equipped.

Folic acid supplements are important for brain heart infusion broth for the cultivation and cloning of Leishmania donovani promastigotes.

Propagation of virulent and avirulent Leishmania donovani promastigotes inhibited by brain heart infusion broth (BHI) (3.7%) are equipped with haemolysed or whole blood (2.5-10%) or heat-inactivated fetal bovine serum (HIFBS) (10-20 %). Peptone, glutamine, tryptophan, purine bases, ribonucleosides, deoxyribonucleosides, B-vitamins and vitamin C were evaluated for reversal of this growth inhibition.

BHI supplementation with folic acid is further complemented by haemolysed rabbit blood or HIFBS and haemin supported serial propagation in subpassages two primary isolates and 3 more virulent strain of L. donovani and 1 strain of L. major. Folic acid is equipped with a 3% BHI blood of rabbits in solid phase with 0.7% agar colony formation also supports L. donovani. This text documents the development of high efficiency media for cultivation of L. donovani promastigotes in the liquid phase and a colony on agar plates using BHI as a basal medium.

BRAIN HEART INFUSION BROTH W/O DEXTROSE

B02-114-2kg 2kg
EUR 380.4

BRAIN HEART INFUSION BROTH W/O DEXTROSE

B02-114-500g 500 g
EUR 146.4

HEART INFUSION BROTH

H08-101-10kg 10 kg
EUR 1351.2

HEART INFUSION BROTH

H08-101-2Kg 2 Kg
EUR 340.8

HEART INFUSION BROTH

H08-101-500g 500 g
EUR 136.8

Brain Heart Infusion

abx082020-100g 100 g
EUR 276

BRAIN HEART INFUSION AGAR

B02-112-10kg 10 kg
EUR 1496.4

BRAIN HEART INFUSION AGAR

B02-112-2kg 2kg
EUR 372

BRAIN HEART INFUSION AGAR

B02-112-500g 500 g
EUR 145.2

Bacto Brain Heart Infusion

237500 EACH
EUR 175.2

BRAIN HEART INFUSION SOLIDS

B02-129-10kg 10 kg
EUR 1203.6

BRAIN HEART INFUSION SOLIDS

B02-129-2Kg 2 Kg
EUR 308.4

BRAIN HEART INFUSION SOLIDS

B02-129-500g 500 g
EUR 124.8

HEART INFUSION AGAR

H08-100-10kg 10 kg
EUR 1399.2

HEART INFUSION AGAR

H08-100-2kg 2kg
EUR 351.6

HEART INFUSION AGAR

H08-100-500g 500 g
EUR 139.2

Brain Hrt Infusion 100gm

237400 EACH
EUR 97.2

Brain Heart Infus 500gm

211059 EACH
EUR 158.4

Brain Heart Infus 500gm

211065 EACH
EUR 212.4

Potato Infusion

41600005-1 100 g
EUR 25.12

Potato Infusion

41600005-2 500 g
EUR 83.08

Potato Infusion

41600005-3 1 kg
EUR 153.37

POTATO INFUSION AGAR

P16-120-10kg 10 kg
EUR 2798.4

POTATO INFUSION AGAR

P16-120-2kg 2kg
EUR 655.2

POTATO INFUSION AGAR

P16-120-500g 500 g
EUR 222

Hrt Infusion Agar 500gm

244400 EACH
EUR 253.2

Heart Inf Broth 500gm

238400 EACH
EUR 267.6

Viral Transport Medium Veal Infusion

IGVTMV1000ML each
EUR 141
Description: Viral Transport Medium Veal Infusion

Viral Transport Medium Veal Infusion

IGVTMV500ML each
EUR 81
Description: Viral Transport Medium Veal Infusion

SURFLO winged infusion set - 19 gauge - cream

DD050164B PK50
EUR 74.4

Brain Heart Inf 2Kg

237200 EACH
EUR 802.8

BHI Broth w 10% Horse Blood

MED1142 PK50
EUR 61.2

LB BROTH - LENNOX LB BROTH

L12-101-10kg 10 kg
EUR 680.4

LB BROTH - LENNOX LB BROTH

L12-101-2kg 2kg
EUR 194.4

LB BROTH - LENNOX LB BROTH

L12-101-500g 500 g
EUR 97.2

LURIA BROTH - MILLER'S LB BROTH

L12-112-10kg 10 kg
EUR 694.8

LURIA BROTH - MILLER'S LB BROTH

L12-112-2kg 2kg
EUR 198

LURIA BROTH - MILLER'S LB BROTH

L12-112-500g 500 g
EUR 97.2

NZ BROTH

N14-110-10kg 10 kg
EUR 1306.8

NZ BROTH

N14-110-2kg 2kg
EUR 331.2

NZ BROTH

N14-110-500g 500 g
EUR 133.2

M BROTH

M13-100-10kg 10 kg
EUR 1554

M BROTH

M13-100-2kg 2kg
EUR 385.2

M BROTH

M13-100-500g 500 g
EUR 148.8

B.H.I. Broth

MED1028 PK50
EUR 52.8

GN BROTH

G07-101-10kg 10 kg
EUR 1293.6

GN BROTH

G07-101-2Kg 2 Kg
EUR 327.6

GN BROTH

G07-101-500g 500 g
EUR 133.2

LB Broth

G246 500 g
EUR 177.6

YM BROTH

Y25-108-10kg 10 kg
EUR 1279.2

YM BROTH

Y25-108-2kg 2kg
EUR 325.2

YM BROTH

Y25-108-500g 500 g
EUR 132

YT Broth

SD7016 250g
EUR 86.1

SF BROTH

S19-109-10kg 10 kg
EUR 2197.2

SF BROTH

S19-109-2Kg 2 Kg
EUR 524.4

SF BROTH

S19-109-500g 500 g
EUR 186

NZM BROTH

N14-104-10kg 10 kg
EUR 962.4

NZM BROTH

N14-104-2kg 2kg
EUR 256.8

NZM BROTH

N14-104-500g 500 g
EUR 112.8

LIM BROTH

L12-129-10kg 10 kg
EUR 944.4

LIM BROTH

L12-129-2Kg 2 Kg
EUR 250.8

LIM BROTH

L12-129-500g 500 g
EUR 111.6

MRS Broth

MED1636 500G
EUR 69.6

YPD BROTH

Y25-103-10kg 10 kg
EUR 963.6

YPD BROTH

Y25-103-2Kg 2 Kg
EUR 256.8

YPD BROTH

Y25-103-500g 500 g
EUR 114

SOB Broth

SD7007 250g
EUR 84.54

SOC Broth

SD7009 250g
EUR 86.1

NZM Broth

SD7014 100g
EUR 73.06

YPD Broth

SD7022 250g
EUR 86.1

TAT BROTH

T20-100-10kg 10 kg
EUR 1393.2

TAT BROTH

T20-100-2Kg 2 Kg
EUR 349.2

TAT BROTH

T20-100-500g 500 g
EUR 139.2

Mh Broth 500gm

211443 EACH
EUR 205.2

Ym Broth 500gm

271120 EACH
EUR 328.8

NZYM BROTH

N14-109-10kg 10 kg
EUR 571.2

NZYM BROTH

N14-109-2Kg 2 Kg
EUR 171.6

NZYM BROTH

N14-109-500g 500 g
EUR 90

Urea Broth

MED1076 PK50
EUR 42

UREA BROTH

U21-102B-10kg 10 kg
EUR 1098

UREA BROTH

U21-102B-2Kg 2 Kg
EUR 285.6

UREA BROTH

U21-102B-500g 500 g
EUR 121.2

HYLB Broth

SD7001 100g
EUR 75.66

NZYM Broth

SD7012 100g
EUR 73.06

2xYT Broth

SD7019 250g
EUR 86.1

TPGY BROTH

T20-127-10kg 10 kg
EUR 1663.2

TPGY BROTH

T20-127-2Kg 2 Kg
EUR 408

TPGY BROTH

T20-127-500g 500 g
EUR 154.8

M17 Broth 500gm

218561 EACH
EUR 306

Duper Broth

D7000-050 500ml
EUR 262.8

NZCYM Broth

SD7010 100g
EUR 75.59

Super Broth

SD7026 250g
EUR 86.1

SUPER BROTH

S19-128-10kg 10 kg
EUR 1005.6

SUPER BROTH

S19-128-2Kg 2 Kg
EUR 265.2

SUPER BROTH

S19-128-500g 500 g
EUR 116.4

BOLTON'S BROTH

B02-132-10kg 10 kg
EUR 2122.8

BOLTON'S BROTH

B02-132-2kg 2kg
EUR 508.8

BOLTON'S BROTH

B02-132-500g 500 g
EUR 182.4

FRASER BROTH

F06-102-10kg 10 kg
EUR 1004.4

FRASER BROTH

F06-102-2Kg 2 Kg
EUR 265.2

FRASER BROTH

F06-102-500g 500 g
EUR 116.4

Fraser Broth

MED1096 PK50
EUR 58.8

Lambda Broth

SD7029 100g
EUR 72.53

NITRATE BROTH

N14-106-10kg 10 kg
EUR 1713.6

NITRATE BROTH

N14-106-2kg 2kg
EUR 420

NITRATE BROTH

N14-106-500g 500 g
EUR 157.2

EUGONIC BROTH

E05-107-10kg 10 kg
EUR 1269.6

EUGONIC BROTH

E05-107-2kg 2kg
EUR 322.8

EUGONIC BROTH

E05-107-500g 500 g
EUR 132

EXTRACT BROTH

E05-109-10kg 10 kg
EUR 60

EXTRACT BROTH

E05-109-2kg 2kg
EUR 60

EXTRACT BROTH

E05-109-500g 500 g
EUR 60

LACTOSE BROTH

L12-105-10kg 10 kg
EUR 931.2

LACTOSE BROTH

L12-105-2kg 2kg
EUR 249.6

LACTOSE BROTH

L12-105-500g 500 g
EUR 111.6

Lactose broth

MED1446 EACH
EUR 88.8

Elliker Broth 500gm

212183 EACH
EUR 457.2

Letheen Broth 500gm

268110 EACH
EUR 375.6

BRUCELLA BROTH

B02-120-10kg 10 kg
EUR 1340.4

BRUCELLA BROTH

B02-120-2Kg 2 Kg
EUR 338.4

BRUCELLA BROTH

B02-120-500g 500 g
EUR 135.6

COLUMBIA BROTH

C03-112-10kg 10 kg
EUR 1372.8

COLUMBIA BROTH

C03-112-2Kg 2 Kg
EUR 345.6

COLUMBIA BROTH

C03-112-500g 500 g
EUR 138

Four Clostridium perfringens strains recovered from clinical specimens, four strains obtained from specimens feces of healthy adults, and the control strain ATCC 13124 was inoculated into Schaedler and Brain Heart Infusion broth, respectively, and incubated anaerobically at 35 degrees C for 24 hours. The exponential growth phase usually lasts from 2 to 6 hours after inoculation; generation times averaging 23 minutes at 2 to 4 hours and 27 minutes between 4 and 6 hours of incubation. The (generation time between 6 and 8 h = 89 minutes) initial growth phase stationary reached at 8 hours after inoculation. All strains shown are essentially identical in vitro growth rate, although they differ in virulence murine.