Clinical comparison of the recoveries of bloodstream pathogens in Septi-Chek brain heart infusion broth with saponin, Septi-Chek tryptic soy broth, and the isolator lysis-centrifugation system.
The recovery of microorganisms in the Septi-Chek biphasic broth tryptic soy (TSB) and Isolator blood culture system as compared to a recovery in Septi-Chek brain biphasic infusion of heart broth is equipped with an agent saponin lytic (L-BHIB; Roche Diagnostics, a Division of Hoffmann-La Roche Inc.). A total of 5,096 TSB-L-BHIB and 1,778 sets of blood culture-L-BHIB Isolator set is evaluated. There are 435 pathogenic organisms recovered in 413 (8.1%) of culture TSB-L-BHIB, with 348 organisms in TSB and 370 bottles in the bottle L-BHIB (P less than 0.05).
Significantly more isolates of yeast found in the system L-BHIB (P less than 0.01). subculture terminal from L-BHIB system is required to detect 13 clinically significant organisms. Significantly more organisms found in a bottle of L-BHIB compared with Isolator system (P less than 0.001), including Torulopsis glabrata, coagulase-negative staphylococci, and Pseudomonas species. The time required to detect all major groups of organisms are the same in both comparisons.
However, the detection of microbial growth in L-BHIB bottle macroscopic examination required sliding unit to be attached and the use of terminal subcultures after 7 days of incubation. The main advantage of L-BHIB biphasic system when compared to either TSB biphasic system or upgraded Isolator system recovery mushrooms.
Nocardia asteroides GUH-2 is not acid-fast when grown in brain heart infusion (BHI) broth. When grown in BHI broth equipped with paraffin, many filamentous cells showed the acid-fastness after treatment with 1% acid-alcohol as a decolorizing agent. When treated with 3% acid-alcohol, the cells not acid-fast filaments. In addition to acid-fast cell filaments of nocardiae, unknown acid-fast round body was observed in the culture broth BHI paraffin-equipped.
Folic acid supplements are important for brain heart infusion broth for the cultivation and cloning of Leishmania donovani promastigotes.
Propagation of virulent and avirulent Leishmania donovani promastigotes inhibited by brain heart infusion broth (BHI) (3.7%) are equipped with haemolysed or whole blood (2.5-10%) or heat-inactivated fetal bovine serum (HIFBS) (10-20 %). Peptone, glutamine, tryptophan, purine bases, ribonucleosides, deoxyribonucleosides, B-vitamins and vitamin C were evaluated for reversal of this growth inhibition.
BHI supplementation with folic acid is further complemented by haemolysed rabbit blood or HIFBS and haemin supported serial propagation in subpassages two primary isolates and 3 more virulent strain of L. donovani and 1 strain of L. major. Folic acid is equipped with a 3% BHI blood of rabbits in solid phase with 0.7% agar colony formation also supports L. donovani. This text documents the development of high efficiency media for cultivation of L. donovani promastigotes in the liquid phase and a colony on agar plates using BHI as a basal medium.
BRAIN HEART INFUSION BROTH W/O DEXTROSE |
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B02-114-2kg | Alphabiosciences | 2kg | EUR 380.4 |
BRAIN HEART INFUSION BROTH W/O DEXTROSE |
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B02-114-500g | Alphabiosciences | 500 g | EUR 146.4 |
HEART INFUSION BROTH |
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H08-101-10kg | Alphabiosciences | 10 kg | EUR 1351.2 |
HEART INFUSION BROTH |
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H08-101-2Kg | Alphabiosciences | 2 Kg | EUR 340.8 |
HEART INFUSION BROTH |
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H08-101-500g | Alphabiosciences | 500 g | EUR 136.8 |
Brain Heart Infusion |
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abx082020-100g | Abbexa | 100 g | EUR 276 |
BRAIN HEART INFUSION AGAR |
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B02-112-10kg | Alphabiosciences | 10 kg | EUR 1496.4 |
BRAIN HEART INFUSION AGAR |
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B02-112-2kg | Alphabiosciences | 2kg | EUR 372 |
BRAIN HEART INFUSION AGAR |
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B02-112-500g | Alphabiosciences | 500 g | EUR 145.2 |
Bacto Brain Heart Infusion |
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237500 | Scientific Laboratory Supplies | EACH | EUR 175.2 |
BRAIN HEART INFUSION SOLIDS |
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B02-129-10kg | Alphabiosciences | 10 kg | EUR 1203.6 |
BRAIN HEART INFUSION SOLIDS |
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B02-129-2Kg | Alphabiosciences | 2 Kg | EUR 308.4 |
BRAIN HEART INFUSION SOLIDS |
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B02-129-500g | Alphabiosciences | 500 g | EUR 124.8 |
HEART INFUSION AGAR |
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H08-100-10kg | Alphabiosciences | 10 kg | EUR 1399.2 |
HEART INFUSION AGAR |
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H08-100-2kg | Alphabiosciences | 2kg | EUR 351.6 |
HEART INFUSION AGAR |
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H08-100-500g | Alphabiosciences | 500 g | EUR 139.2 |
Brain Hrt Infusion 100gm |
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237400 | Scientific Laboratory Supplies | EACH | EUR 97.2 |
Brain Heart Infus 500gm |
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211059 | Scientific Laboratory Supplies | EACH | EUR 158.4 |
Brain Heart Infus 500gm |
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211065 | Scientific Laboratory Supplies | EACH | EUR 212.4 |
Potato Infusion |
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41600005-1 | Bio-WORLD | 100 g | EUR 25.12 |
Potato Infusion |
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41600005-2 | Bio-WORLD | 500 g | EUR 83.08 |
Potato Infusion |
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41600005-3 | Bio-WORLD | 1 kg | EUR 153.37 |
POTATO INFUSION AGAR |
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P16-120-10kg | Alphabiosciences | 10 kg | EUR 2798.4 |
POTATO INFUSION AGAR |
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P16-120-2kg | Alphabiosciences | 2kg | EUR 655.2 |
POTATO INFUSION AGAR |
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P16-120-500g | Alphabiosciences | 500 g | EUR 222 |
Hrt Infusion Agar 500gm |
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244400 | Scientific Laboratory Supplies | EACH | EUR 253.2 |
Heart Inf Broth 500gm |
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238400 | Scientific Laboratory Supplies | EACH | EUR 267.6 |
Viral Transport Medium Veal Infusion |
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IGVTMV1000ML | Innovative research | each | EUR 141 |
Description: Viral Transport Medium Veal Infusion |
Viral Transport Medium Veal Infusion |
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IGVTMV500ML | Innovative research | each | EUR 81 |
Description: Viral Transport Medium Veal Infusion |
SURFLO winged infusion set - 19 gauge - cream |
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DD050164B | Scientific Laboratory Supplies | PK50 | EUR 74.4 |
Brain Heart Inf 2Kg |
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237200 | Scientific Laboratory Supplies | EACH | EUR 802.8 |
BHI Broth w 10% Horse Blood |
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MED1142 | Scientific Laboratory Supplies | PK50 | EUR 61.2 |
LB BROTH - LENNOX LB BROTH |
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L12-101-10kg | Alphabiosciences | 10 kg | EUR 680.4 |
LB BROTH - LENNOX LB BROTH |
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L12-101-2kg | Alphabiosciences | 2kg | EUR 194.4 |
LB BROTH - LENNOX LB BROTH |
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L12-101-500g | Alphabiosciences | 500 g | EUR 97.2 |
LURIA BROTH - MILLER'S LB BROTH |
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L12-112-10kg | Alphabiosciences | 10 kg | EUR 694.8 |
LURIA BROTH - MILLER'S LB BROTH |
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L12-112-2kg | Alphabiosciences | 2kg | EUR 198 |
LURIA BROTH - MILLER'S LB BROTH |
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L12-112-500g | Alphabiosciences | 500 g | EUR 97.2 |
NZ BROTH |
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N14-110-10kg | Alphabiosciences | 10 kg | EUR 1306.8 |
NZ BROTH |
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N14-110-2kg | Alphabiosciences | 2kg | EUR 331.2 |
NZ BROTH |
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N14-110-500g | Alphabiosciences | 500 g | EUR 133.2 |
M BROTH |
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M13-100-10kg | Alphabiosciences | 10 kg | EUR 1554 |
M BROTH |
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M13-100-2kg | Alphabiosciences | 2kg | EUR 385.2 |
M BROTH |
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M13-100-500g | Alphabiosciences | 500 g | EUR 148.8 |
B.H.I. Broth |
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MED1028 | Scientific Laboratory Supplies | PK50 | EUR 52.8 |
GN BROTH |
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G07-101-10kg | Alphabiosciences | 10 kg | EUR 1293.6 |
GN BROTH |
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G07-101-2Kg | Alphabiosciences | 2 Kg | EUR 327.6 |
GN BROTH |
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G07-101-500g | Alphabiosciences | 500 g | EUR 133.2 |
LB Broth |
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G246 | ABM | 500 g | EUR 177.6 |
YM BROTH |
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Y25-108-10kg | Alphabiosciences | 10 kg | EUR 1279.2 |
YM BROTH |
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Y25-108-2kg | Alphabiosciences | 2kg | EUR 325.2 |
YM BROTH |
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Y25-108-500g | Alphabiosciences | 500 g | EUR 132 |
YT Broth |
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SD7016 | Bio Basic | 250g | EUR 86.1 |
SF BROTH |
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S19-109-10kg | Alphabiosciences | 10 kg | EUR 2197.2 |
SF BROTH |
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S19-109-2Kg | Alphabiosciences | 2 Kg | EUR 524.4 |
SF BROTH |
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S19-109-500g | Alphabiosciences | 500 g | EUR 186 |
NZM BROTH |
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N14-104-10kg | Alphabiosciences | 10 kg | EUR 962.4 |
NZM BROTH |
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N14-104-2kg | Alphabiosciences | 2kg | EUR 256.8 |
NZM BROTH |
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N14-104-500g | Alphabiosciences | 500 g | EUR 112.8 |
LIM BROTH |
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L12-129-10kg | Alphabiosciences | 10 kg | EUR 944.4 |
LIM BROTH |
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L12-129-2Kg | Alphabiosciences | 2 Kg | EUR 250.8 |
LIM BROTH |
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L12-129-500g | Alphabiosciences | 500 g | EUR 111.6 |
MRS Broth |
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MED1636 | Scientific Laboratory Supplies | 500G | EUR 69.6 |
YPD BROTH |
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Y25-103-10kg | Alphabiosciences | 10 kg | EUR 963.6 |
YPD BROTH |
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Y25-103-2Kg | Alphabiosciences | 2 Kg | EUR 256.8 |
YPD BROTH |
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Y25-103-500g | Alphabiosciences | 500 g | EUR 114 |
SOB Broth |
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SD7007 | Bio Basic | 250g | EUR 84.54 |
SOC Broth |
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SD7009 | Bio Basic | 250g | EUR 86.1 |
NZM Broth |
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SD7014 | Bio Basic | 100g | EUR 73.06 |
YPD Broth |
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SD7022 | Bio Basic | 250g | EUR 86.1 |
TAT BROTH |
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T20-100-10kg | Alphabiosciences | 10 kg | EUR 1393.2 |
TAT BROTH |
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T20-100-2Kg | Alphabiosciences | 2 Kg | EUR 349.2 |
TAT BROTH |
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T20-100-500g | Alphabiosciences | 500 g | EUR 139.2 |
Mh Broth 500gm |
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211443 | Scientific Laboratory Supplies | EACH | EUR 205.2 |
Ym Broth 500gm |
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271120 | Scientific Laboratory Supplies | EACH | EUR 328.8 |
NZYM BROTH |
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N14-109-10kg | Alphabiosciences | 10 kg | EUR 571.2 |
NZYM BROTH |
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N14-109-2Kg | Alphabiosciences | 2 Kg | EUR 171.6 |
NZYM BROTH |
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N14-109-500g | Alphabiosciences | 500 g | EUR 90 |
Urea Broth |
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MED1076 | Scientific Laboratory Supplies | PK50 | EUR 42 |
UREA BROTH |
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U21-102B-10kg | Alphabiosciences | 10 kg | EUR 1098 |
UREA BROTH |
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U21-102B-2Kg | Alphabiosciences | 2 Kg | EUR 285.6 |
UREA BROTH |
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U21-102B-500g | Alphabiosciences | 500 g | EUR 121.2 |
HYLB Broth |
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SD7001 | Bio Basic | 100g | EUR 75.66 |
NZYM Broth |
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SD7012 | Bio Basic | 100g | EUR 73.06 |
2xYT Broth |
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SD7019 | Bio Basic | 250g | EUR 86.1 |
TPGY BROTH |
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T20-127-10kg | Alphabiosciences | 10 kg | EUR 1663.2 |
TPGY BROTH |
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T20-127-2Kg | Alphabiosciences | 2 Kg | EUR 408 |
TPGY BROTH |
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T20-127-500g | Alphabiosciences | 500 g | EUR 154.8 |
M17 Broth 500gm |
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218561 | Scientific Laboratory Supplies | EACH | EUR 306 |
Duper Broth |
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D7000-050 | GenDepot | 500ml | EUR 262.8 |
NZCYM Broth |
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SD7010 | Bio Basic | 100g | EUR 75.59 |
Super Broth |
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SD7026 | Bio Basic | 250g | EUR 86.1 |
SUPER BROTH |
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S19-128-10kg | Alphabiosciences | 10 kg | EUR 1005.6 |
SUPER BROTH |
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S19-128-2Kg | Alphabiosciences | 2 Kg | EUR 265.2 |
SUPER BROTH |
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S19-128-500g | Alphabiosciences | 500 g | EUR 116.4 |
BOLTON'S BROTH |
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B02-132-10kg | Alphabiosciences | 10 kg | EUR 2122.8 |
BOLTON'S BROTH |
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B02-132-2kg | Alphabiosciences | 2kg | EUR 508.8 |
BOLTON'S BROTH |
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B02-132-500g | Alphabiosciences | 500 g | EUR 182.4 |
FRASER BROTH |
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F06-102-10kg | Alphabiosciences | 10 kg | EUR 1004.4 |
FRASER BROTH |
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F06-102-2Kg | Alphabiosciences | 2 Kg | EUR 265.2 |
FRASER BROTH |
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F06-102-500g | Alphabiosciences | 500 g | EUR 116.4 |
Fraser Broth |
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MED1096 | Scientific Laboratory Supplies | PK50 | EUR 58.8 |
Lambda Broth |
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SD7029 | Bio Basic | 100g | EUR 72.53 |
NITRATE BROTH |
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N14-106-10kg | Alphabiosciences | 10 kg | EUR 1713.6 |
NITRATE BROTH |
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N14-106-2kg | Alphabiosciences | 2kg | EUR 420 |
NITRATE BROTH |
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N14-106-500g | Alphabiosciences | 500 g | EUR 157.2 |
EUGONIC BROTH |
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E05-107-10kg | Alphabiosciences | 10 kg | EUR 1269.6 |
EUGONIC BROTH |
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E05-107-2kg | Alphabiosciences | 2kg | EUR 322.8 |
EUGONIC BROTH |
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E05-107-500g | Alphabiosciences | 500 g | EUR 132 |
EXTRACT BROTH |
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E05-109-10kg | Alphabiosciences | 10 kg | EUR 60 |
EXTRACT BROTH |
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E05-109-2kg | Alphabiosciences | 2kg | EUR 60 |
EXTRACT BROTH |
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E05-109-500g | Alphabiosciences | 500 g | EUR 60 |
LACTOSE BROTH |
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L12-105-10kg | Alphabiosciences | 10 kg | EUR 931.2 |
LACTOSE BROTH |
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L12-105-2kg | Alphabiosciences | 2kg | EUR 249.6 |
LACTOSE BROTH |
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L12-105-500g | Alphabiosciences | 500 g | EUR 111.6 |
Lactose broth |
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MED1446 | Scientific Laboratory Supplies | EACH | EUR 88.8 |
Elliker Broth 500gm |
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212183 | Scientific Laboratory Supplies | EACH | EUR 457.2 |
Letheen Broth 500gm |
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268110 | Scientific Laboratory Supplies | EACH | EUR 375.6 |
BRUCELLA BROTH |
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B02-120-10kg | Alphabiosciences | 10 kg | EUR 1340.4 |
BRUCELLA BROTH |
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B02-120-2Kg | Alphabiosciences | 2 Kg | EUR 338.4 |
BRUCELLA BROTH |
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B02-120-500g | Alphabiosciences | 500 g | EUR 135.6 |
COLUMBIA BROTH |
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C03-112-10kg | Alphabiosciences | 10 kg | EUR 1372.8 |
COLUMBIA BROTH |
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C03-112-2Kg | Alphabiosciences | 2 Kg | EUR 345.6 |
COLUMBIA BROTH |
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C03-112-500g | Alphabiosciences | 500 g | EUR 138 |
Four Clostridium perfringens strains recovered from clinical specimens, four strains obtained from specimens feces of healthy adults, and the control strain ATCC 13124 was inoculated into Schaedler and Brain Heart Infusion broth, respectively, and incubated anaerobically at 35 degrees C for 24 hours. The exponential growth phase usually lasts from 2 to 6 hours after inoculation; generation times averaging 23 minutes at 2 to 4 hours and 27 minutes between 4 and 6 hours of incubation. The (generation time between 6 and 8 h = 89 minutes) initial growth phase stationary reached at 8 hours after inoculation. All strains shown are essentially identical in vitro growth rate, although they differ in virulence murine.