Clinical comparison of the recoveries of bloodstream pathogens in Septi-Chek brain heart infusion broth with saponin, Septi-Chek tryptic soy broth, and the isolator lysis-centrifugation system.
The recovery of microorganisms in the Septi-Chek biphasic broth tryptic soy (TSB) and Isolator blood culture system as compared to a recovery in Septi-Chek brain biphasic infusion of heart broth is equipped with an agent saponin lytic (L-BHIB; Roche Diagnostics, a Division of Hoffmann-La Roche Inc.). A total of 5,096 TSB-L-BHIB and 1,778 sets of blood culture-L-BHIB Isolator set is evaluated. There are 435 pathogenic organisms recovered in 413 (8.1%) of culture TSB-L-BHIB, with 348 organisms in TSB and 370 bottles in the bottle L-BHIB (P less than 0.05).
Significantly more isolates of yeast found in the system L-BHIB (P less than 0.01). subculture terminal from L-BHIB system is required to detect 13 clinically significant organisms. Significantly more organisms found in a bottle of L-BHIB compared with Isolator system (P less than 0.001), including Torulopsis glabrata, coagulase-negative staphylococci, and Pseudomonas species. The time required to detect all major groups of organisms are the same in both comparisons.
However, the detection of microbial growth in L-BHIB bottle macroscopic examination required sliding unit to be attached and the use of terminal subcultures after 7 days of incubation. The main advantage of L-BHIB biphasic system when compared to either TSB biphasic system or upgraded Isolator system recovery mushrooms.
Nocardia asteroides GUH-2 is not acid-fast when grown in brain heart infusion (BHI) broth. When grown in BHI broth equipped with paraffin, many filamentous cells showed the acid-fastness after treatment with 1% acid-alcohol as a decolorizing agent. When treated with 3% acid-alcohol, the cells not acid-fast filaments. In addition to acid-fast cell filaments of nocardiae, unknown acid-fast round body was observed in the culture broth BHI paraffin-equipped.
Folic acid supplements are important for brain heart infusion broth for the cultivation and cloning of Leishmania donovani promastigotes.
Propagation of virulent and avirulent Leishmania donovani promastigotes inhibited by brain heart infusion broth (BHI) (3.7%) are equipped with haemolysed or whole blood (2.5-10%) or heat-inactivated fetal bovine serum (HIFBS) (10-20 %). Peptone, glutamine, tryptophan, purine bases, ribonucleosides, deoxyribonucleosides, B-vitamins and vitamin C were evaluated for reversal of this growth inhibition.
BHI supplementation with folic acid is further complemented by haemolysed rabbit blood or HIFBS and haemin supported serial propagation in subpassages two primary isolates and 3 more virulent strain of L. donovani and 1 strain of L. major. Folic acid is equipped with a 3% BHI blood of rabbits in solid phase with 0.7% agar colony formation also supports L. donovani. This text documents the development of high efficiency media for cultivation of L. donovani promastigotes in the liquid phase and a colony on agar plates using BHI as a basal medium.
BRAIN HEART INFUSION BROTH W/O DEXTROSE |
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B02-114-500g | Alphabiosciences | 500 g | EUR 146.4 |
Brain Heart Infusion (BHI)Agar - EACH |
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MED1432 | Scientific Laboratory Supplies | EACH | EUR 113.4 |
BHI HiVeg™ w/PABA (Brain Heart Infusion |
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MV212-500G | EWC Diagnostics | 1 unit | EUR 45.83 |
Description: BHI HiVeg™ w/PABA (Brain Heart Infusion |
HEART INFUSION BROTH |
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H08-101-10kg | Alphabiosciences | 10 kg | EUR 1351.2 |
HEART INFUSION BROTH |
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H08-101-2Kg | Alphabiosciences | 2 Kg | EUR 340.8 |
HEART INFUSION BROTH |
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H08-101-500g | Alphabiosciences | 500 g | EUR 136.8 |
Brain Heart Infusion |
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abx082020-100g | Abbexa | 100 g | EUR 276 |
Brain Heart Infusion |
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abx082020-100l | Abbexa | 100 µl | EUR 237.5 |
Brain Heart Infusion |
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abx082020-1ml | Abbexa | 1 ml | Ask for price |
Brain Heart Infusion |
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abx082020-200l | Abbexa | 200 µl | Ask for price |
BRAIN HEART INFUSION AGAR |
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B02-112-10kg | Alphabiosciences | 10 kg | EUR 1496.4 |
BRAIN HEART INFUSION AGAR |
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B02-112-2kg | Alphabiosciences | 2kg | EUR 372 |
BRAIN HEART INFUSION AGAR |
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B02-112-500g | Alphabiosciences | 500 g | EUR 145.2 |
BRAIN HEART INFUSION SOLIDS |
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B02-129-10kg | Alphabiosciences | 10 kg | EUR 1203.6 |
BRAIN HEART INFUSION SOLIDS |
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B02-129-2Kg | Alphabiosciences | 2 Kg | EUR 308.4 |
BRAIN HEART INFUSION SOLIDS |
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B02-129-500g | Alphabiosciences | 500 g | EUR 124.8 |
V Infusion Broth |
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M329-500G | EWC Diagnostics | 1 unit | EUR 43.27 |
Description: V Infusion Broth |
Potato Infusion Broth |
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M1833-500G | EWC Diagnostics | 1 unit | EUR 40.51 |
Description: Potato Infusion Broth |
HEART INFUSION AGAR |
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H08-100-10kg | Alphabiosciences | 10 kg | EUR 1399.2 |
HEART INFUSION AGAR |
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H08-100-2kg | Alphabiosciences | 2kg | EUR 351.6 |
HEART INFUSION AGAR |
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H08-100-500g | Alphabiosciences | 500 g | EUR 139.2 |
Brain Hrt Infusion 100gm - EACH |
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237400 | Scientific Laboratory Supplies | EACH | EUR 109.35 |
BHI Agar (Special Infusion Agar) |
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M211-100G | EWC Diagnostics | 1 unit | EUR 13.42 |
Description: BHI Agar (Special Infusion Agar) |
BHI Agar (Special Infusion Agar) |
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M211-500G | EWC Diagnostics | 1 unit | EUR 33.1 |
Description: BHI Agar (Special Infusion Agar) |
BHI Agar (Special Infusion Agar), Granul |
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GM211-500G | EWC Diagnostics | 1 unit | EUR 38.09 |
Description: BHI Agar (Special Infusion Agar), Granul |
BHI HiCynth™ Agar ( Special Infusion HiC |
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MCD211-100G | EWC Diagnostics | 1 unit | EUR 15.46 |
Description: BHI HiCynth™ Agar ( Special Infusion HiC |
BHI HiCynth™ Agar ( Special Infusion HiC |
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MCD211-500G | EWC Diagnostics | 1 unit | EUR 38.09 |
Description: BHI HiCynth™ Agar ( Special Infusion HiC |
Brain Heart Infus 500gm |
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211065 | Scientific Laboratory Supplies | EACH | EUR 201.78 |
Brain Heart Infus 500gm - EACH |
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211059 | Scientific Laboratory Supplies | EACH | EUR 178.2 |
Potato Infusion |
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41600005-1 | Glycomatrix | 100 g | EUR 25.12 |
Potato Infusion |
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41600005-2 | Glycomatrix | 500 g | EUR 83.08 |
Potato Infusion |
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41600005-3 | Glycomatrix | 1 kg | EUR 153.37 |
V Infusion Agar |
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M328-500G | EWC Diagnostics | 1 unit | EUR 46.08 |
Description: V Infusion Agar |
LM Infusion Agar |
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M1206-500G | EWC Diagnostics | 1 unit | EUR 42.8 |
Description: LM Infusion Agar |
HiVeg™ Infusion |
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RM191V-500G | EWC Diagnostics | 1 unit | EUR 49.58 |
Description: HiVeg™ Infusion |
HM Infusion Powder |
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RM192-500G | EWC Diagnostics | 1 unit | EUR 34.52 |
Description: HM Infusion Powder |
HL Infusion Powder |
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RM022-500G | EWC Diagnostics | 1 unit | EUR 34.17 |
Description: HL Infusion Powder |
POTATO INFUSION AGAR |
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P16-120-10kg | Alphabiosciences | 10 kg | EUR 2798.4 |
POTATO INFUSION AGAR |
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P16-120-2kg | Alphabiosciences | 2kg | EUR 655.2 |
POTATO INFUSION AGAR |
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P16-120-500g | Alphabiosciences | 500 g | EUR 222 |
Potato Infusion Agar |
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M174-500G | EWC Diagnostics | 1 unit | EUR 38.38 |
Description: Potato Infusion Agar |
Standard Infusion Agar |
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M883-500G | EWC Diagnostics | 1 unit | EUR 42.8 |
Description: Standard Infusion Agar |
HiVeg# Special Infusion |
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RM188V-500G | EWC Diagnostics | 1 unit | EUR 38.25 |
Description: HiVeg# Special Infusion |
LM Infusion HiVeg Agar |
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MV1206-500G | EWC Diagnostics | 1 unit | EUR 42.8 |
Description: LM Infusion HiVeg Agar |
HiVeg™ Infusion No. 2 |
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RM192V-500G | EWC Diagnostics | 1 unit | EUR 35.48 |
Description: HiVeg™ Infusion No. 2 |
HiVeg™ Infusion No. 1 |
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RM022V-500G | EWC Diagnostics | 1 unit | EUR 35.37 |
Description: HiVeg™ Infusion No. 1 |
Potato Infusion HiVeg Agar |
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MV174-500G | EWC Diagnostics | 1 unit | EUR 38.38 |
Description: Potato Infusion HiVeg Agar |
Standard Infusion Agar, HiVeg |
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MV883-500G | EWC Diagnostics | 1 unit | EUR 42.8 |
Description: Standard Infusion Agar, HiVeg |
Hrt Infusion Agar 500gm - EACH |
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244400 | Scientific Laboratory Supplies | EACH | EUR 284.85 |
Blood Agar Base (Infusion Agar) |
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GM073-100G | EWC Diagnostics | 1 unit | EUR 12.97 |
Description: Blood Agar Base (Infusion Agar) |
Blood Agar Base (Infusion Agar) |
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GM073-500G | EWC Diagnostics | 1 unit | EUR 38.09 |
Description: Blood Agar Base (Infusion Agar) |
Blood Agar Base (Infusion Agar) |
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M073-100G | EWC Diagnostics | 1 unit | EUR 13.42 |
Description: Blood Agar Base (Infusion Agar) |
Blood Agar Base (Infusion Agar) |
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M073-500G | EWC Diagnostics | 1 unit | EUR 33.1 |
Description: Blood Agar Base (Infusion Agar) |
VEAL INFUSION VIRAL TRANSPORT MEDIUM |
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7574002 | Lampire Biologicals Laboratories | 1ML | EUR 59.77 |
Viral Transport Medium Veal Infusion |
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IGVTMV1000ML | Innovative research | each | EUR 141 |
Description: Viral Transport Medium Veal Infusion |
Viral Transport Medium Veal Infusion |
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IGVTMV500ML | Innovative research | each | EUR 81 |
Description: Viral Transport Medium Veal Infusion |
Blood Agar Base, HiVeg (Infusion Agar, |
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MV073-100G | EWC Diagnostics | 1 unit | EUR 13.42 |
Description: Blood Agar Base, HiVeg (Infusion Agar, |
Blood Agar Base, HiVeg (Infusion Agar, |
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MV073-500G | EWC Diagnostics | 1 unit | EUR 33.1 |
Description: Blood Agar Base, HiVeg (Infusion Agar, |
Blood HiCynth Agar Base (Infusion HiCynt |
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MCD073-100G | EWC Diagnostics | 1 unit | EUR 15.46 |
Description: Blood HiCynth Agar Base (Infusion HiCynt |
Blood HiCynth Agar Base (Infusion HiCynt |
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MCD073-500G | EWC Diagnostics | 1 unit | EUR 38.09 |
Description: Blood HiCynth Agar Base (Infusion HiCynt |
VEAL INFUSION VIRAL TRANSPORT MEDIUM, 1LTR |
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7574002-1LTR | Lampire Biologicals Laboratories | 1LTR | EUR 111.02 |
VEAL INFUSION VIRAL TRANSPORT MEDIUM, 1 ML |
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7574002-A | Lampire Biologicals Laboratories | 1ML | EUR 14.39 |
SURFLO winged infusion set - 19 gauge - cream - PK50 |
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DD050164B | Scientific Laboratory Supplies | PK50 | EUR 83.7 |
BHI Broth |
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M210-100G | EWC Diagnostics | 1 unit | EUR 13.42 |
Description: BHI Broth |
BHI Broth |
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M210-500G | EWC Diagnostics | 1 unit | EUR 33.1 |
Description: BHI Broth |
BHI Broth |
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M210I-100G | EWC Diagnostics | 1 unit | EUR 15.36 |
Description: BHI Broth |
BHI Broth |
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M210I-500G | EWC Diagnostics | 1 unit | EUR 39.2 |
Description: BHI Broth |
BHI Broth |
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LQ077-25X5ML | EWC Diagnostics | 1 unit | EUR 10.75 |
Description: BHI Broth |
BHI Broth |
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LQ077-50X5ML | EWC Diagnostics | 1 unit | EUR 16.75 |
Description: BHI Broth |
BHI Broth |
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LQ077V-25X5ML | EWC Diagnostics | 1 unit | EUR 11.18 |
Description: BHI Broth |
BHI Broth |
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LQ210C-5X100ML | EWC Diagnostics | 1 unit | EUR 8.52 |
Description: BHI Broth |
BHI Broth |
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LQ210D-5X500ML | EWC Diagnostics | 1 unit | EUR 22.11 |
Description: BHI Broth |
Brain Heart Inf 2Kg - EACH |
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237200 | Scientific Laboratory Supplies | EACH | EUR 903.15 |
BHI Broth, Granulated |
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GM210-500G | EWC Diagnostics | 1 unit | EUR 38.09 |
Description: BHI Broth, Granulated |
BHI Broth, HiVeg™ |
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MV210-100G | EWC Diagnostics | 1 unit | EUR 13.42 |
Description: BHI Broth, HiVeg™ |
BHI Broth, HiVeg™ |
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MV210-500G | EWC Diagnostics | 1 unit | EUR 33.1 |
Description: BHI Broth, HiVeg™ |
BHI HiCynth™ Broth |
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MCD210-100G | EWC Diagnostics | 1 unit | EUR 15.46 |
Description: BHI HiCynth™ Broth |
BHI HiCynth™ Broth |
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MCD210-500G | EWC Diagnostics | 1 unit | EUR 38.09 |
Description: BHI HiCynth™ Broth |
HiEncap™ BHI Broth |
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EC210CCL-25NO | EWC Diagnostics | 1 unit | EUR 58.98 |
Description: HiEncap™ BHI Broth |
HiEncap™ BHI Broth |
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EC210D-10NO | EWC Diagnostics | 1 unit | EUR 39.54 |
Description: HiEncap™ BHI Broth |
BHI Broth (Gamma Irradiated) |
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M210G-500G | EWC Diagnostics | 1 unit | EUR 40.82 |
Description: BHI Broth (Gamma Irradiated) |
BHI Broth w/o Dextrose |
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M5432-100G | EWC Diagnostics | 1 unit | EUR 24.87 |
Description: BHI Broth w/o Dextrose |
BHI Broth w/o Dextrose |
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M5432-500G | EWC Diagnostics | 1 unit | EUR 104.71 |
Description: BHI Broth w/o Dextrose |
BHI Broth w 10% Horse Blood - PK50 |
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MED1142 | Scientific Laboratory Supplies | PK50 | EUR 68.85 |
YPD Broth (YEPD Broth) |
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GM1363-500G | EWC Diagnostics | 1 unit | EUR 60.05 |
Description: YPD Broth (YEPD Broth) |
YPD Broth (YEPD Broth) |
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M1363-500G | EWC Diagnostics | 1 unit | EUR 52.25 |
Description: YPD Broth (YEPD Broth) |
YT Broth (2X YT Broth) |
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GM1251-500G | EWC Diagnostics | 1 unit | EUR 50.84 |
Description: YT Broth (2X YT Broth) |
LB BROTH - LENNOX LB BROTH |
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L12-101-10kg | Alphabiosciences | 10 kg | EUR 680.4 |
LB BROTH - LENNOX LB BROTH |
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L12-101-2kg | Alphabiosciences | 2kg | EUR 194.4 |
LB BROTH - LENNOX LB BROTH |
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L12-101-500g | Alphabiosciences | 500 g | EUR 97.2 |
Yeast Malt Broth (YM Broth) |
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M425-100G | EWC Diagnostics | 1 unit | EUR 14.77 |
Description: Yeast Malt Broth (YM Broth) |
Yeast Malt Broth (YM Broth) |
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M425-500G | EWC Diagnostics | 1 unit | EUR 38.04 |
Description: Yeast Malt Broth (YM Broth) |
LURIA BROTH - MILLER'S LB BROTH |
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L12-112-10kg | Alphabiosciences | 10 kg | EUR 694.8 |
LURIA BROTH - MILLER'S LB BROTH |
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L12-112-2kg | Alphabiosciences | 2kg | EUR 198 |
LURIA BROTH - MILLER'S LB BROTH |
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L12-112-500g | Alphabiosciences | 500 g | EUR 97.2 |
Feeley Gorman Broth (F.G. Broth) |
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M812-500G | EWC Diagnostics | 1 unit | EUR 43.37 |
Description: Feeley Gorman Broth (F.G. Broth) |
Lactose Broth (Broth Medium D) |
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ME1003-100G | EWC Diagnostics | 1 unit | EUR 10.99 |
Description: Lactose Broth (Broth Medium D) |
Lactose Broth (Broth Medium D) |
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ME1003-500G | EWC Diagnostics | 1 unit | EUR 25.97 |
Description: Lactose Broth (Broth Medium D) |
Lactose Broth (Broth Medium D) |
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M1003B-100G | EWC Diagnostics | 1 unit | EUR 10.99 |
Description: Lactose Broth (Broth Medium D) |
Lactose Broth (Broth Medium D) |
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M1003B-500G | EWC Diagnostics | 1 unit | EUR 25.97 |
Description: Lactose Broth (Broth Medium D) |
*Lactobacillus MRS Broth (MRS Broth) |
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GM369-100G | EWC Diagnostics | 1 unit | EUR 18.33 |
Description: *Lactobacillus MRS Broth (MRS Broth) |
*Lactobacillus MRS Broth (MRS Broth) |
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GM369-500G | EWC Diagnostics | 1 unit | EUR 46.19 |
Description: *Lactobacillus MRS Broth (MRS Broth) |
Lactose Sulphite Broth Base (Broth |
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M1287B-500G | EWC Diagnostics | 1 unit | EUR 34.17 |
Description: Lactose Sulphite Broth Base (Broth |
Tartoff - Hobbs Broth (Terrific Broth) |
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GM1250-10KG | EWC Diagnostics | 1 unit | EUR 1260.67 |
Description: Tartoff - Hobbs Broth (Terrific Broth) |
Tartoff - Hobbs Broth (Terrific Broth) |
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GM1250-500G | EWC Diagnostics | 1 unit | EUR 64.25 |
Description: Tartoff - Hobbs Broth (Terrific Broth) |
Casitose Magnesium Broth (NZM Broth) |
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M1249-500G | EWC Diagnostics | 1 unit | EUR 55.11 |
Description: Casitose Magnesium Broth (NZM Broth) |
Tartoff - Hobbs Broth (Terrific Broth) |
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M1250-500G | EWC Diagnostics | 1 unit | EUR 55.87 |
Description: Tartoff - Hobbs Broth (Terrific Broth) |
Tartoff - Hobbs Broth (Terrific Broth) |
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M1250G-500G | EWC Diagnostics | 1 unit | EUR 67.44 |
Description: Tartoff - Hobbs Broth (Terrific Broth) |
AATCC Bacteriostasis Broth (FDA Broth) |
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M221-500G | EWC Diagnostics | 1 unit | EUR 44.15 |
Description: AATCC Bacteriostasis Broth (FDA Broth) |
Ethyl Violet Azide Broth (E.V.A. Broth) |
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M426-500G | EWC Diagnostics | 1 unit | EUR 36.99 |
Description: Ethyl Violet Azide Broth (E.V.A. Broth) |
Ethyl Violet Azide Broth (E.V.A. Broth) |
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M426S-100G | EWC Diagnostics | 1 unit | EUR 15.93 |
Description: Ethyl Violet Azide Broth (E.V.A. Broth) |
Ethyl Violet Azide Broth (E.V.A. Broth) |
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M426S-500G | EWC Diagnostics | 1 unit | EUR 42.55 |
Description: Ethyl Violet Azide Broth (E.V.A. Broth) |
Selenite Broth (Selenite F Broth) (Twin |
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GM052-500G | EWC Diagnostics | 1 unit | EUR 54.52 |
Description: Selenite Broth (Selenite F Broth) (Twin |
MR-VP Broth (Glucose Phosphate Broth) |
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LQ082-25X5ML | EWC Diagnostics | 1 unit | EUR 10.27 |
Description: MR-VP Broth (Glucose Phosphate Broth) |
MR-VP Broth (Glucose Phosphate Broth) |
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LQ082-50X5ML | EWC Diagnostics | 1 unit | EUR 15.93 |
Description: MR-VP Broth (Glucose Phosphate Broth) |
Selenite Broth (Selenite F Broth) (Twin |
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M052-100G | EWC Diagnostics | 1 unit | EUR 17.21 |
Description: Selenite Broth (Selenite F Broth) (Twin |
Selenite Broth (Selenite F Broth) (Twin |
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M052-500G | EWC Diagnostics | 1 unit | EUR 47.43 |
Description: Selenite Broth (Selenite F Broth) (Twin |
ITC HiVeg Broth Base (TTC HiVeg Broth |
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MV1220-500G | EWC Diagnostics | 1 unit | EUR 52.25 |
Description: ITC HiVeg Broth Base (TTC HiVeg Broth |
Yeast Malt HiVeg Broth (YM HiVeg Broth) |
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MV425-100G | EWC Diagnostics | 1 unit | EUR 14.77 |
Description: Yeast Malt HiVeg Broth (YM HiVeg Broth) |
Yeast Malt HiVeg Broth (YM HiVeg Broth) |
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MV425-500G | EWC Diagnostics | 1 unit | EUR 38.04 |
Description: Yeast Malt HiVeg Broth (YM HiVeg Broth) |
cDNA - Congenital heart disease: Heart |
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C1236122Hd-3 | Biochain | 40 reactions | EUR 802 |
cDNA - Congestive Heart Failure: Heart |
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C1236122Hd-6 | Biochain | 40 reactions | EUR 989 |
Heart (Congenital heart disease) Lysate |
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XBL-10342 | ProSci | 0.1 mg | EUR 796.2 |
Description: Human heart tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human heart tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the heart tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The heart tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
GN BROTH |
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G07-101-10kg | Alphabiosciences | 10 kg | EUR 1293.6 |
Four Clostridium perfringens strains recovered from clinical specimens, four strains obtained from specimens feces of healthy adults, and the control strain ATCC 13124 was inoculated into Schaedler and Brain Heart Infusion broth, respectively, and incubated anaerobically at 35 degrees C for 24 hours. The exponential growth phase usually lasts from 2 to 6 hours after inoculation; generation times averaging 23 minutes at 2 to 4 hours and 27 minutes between 4 and 6 hours of incubation. The (generation time between 6 and 8 h = 89 minutes) initial growth phase stationary reached at 8 hours after inoculation. All strains shown are essentially identical in vitro growth rate, although they differ in virulence murine.