Clinical comparison of the recoveries of bloodstream pathogens in Septi-Chek brain heart infusion broth with saponin, Septi-Chek tryptic soy broth, and the isolator lysis-centrifugation system.

The recovery of microorganisms in the Septi-Chek biphasic broth tryptic soy (TSB) and Isolator blood culture system as compared to a recovery in Septi-Chek brain biphasic infusion of heart broth is equipped with an agent saponin lytic (L-BHIB; Roche Diagnostics, a Division of Hoffmann-La Roche Inc.). A total of 5,096 TSB-L-BHIB and 1,778 sets of blood culture-L-BHIB Isolator set is evaluated. There are 435 pathogenic organisms recovered in 413 (8.1%) of culture TSB-L-BHIB, with 348 organisms in TSB and 370 bottles in the bottle L-BHIB (P less than 0.05).

Significantly more isolates of yeast found in the system L-BHIB (P less than 0.01). subculture terminal from L-BHIB system is required to detect 13 clinically significant organisms. Significantly more organisms found in a bottle of L-BHIB compared with Isolator system (P less than 0.001), including Torulopsis glabrata, coagulase-negative staphylococci, and Pseudomonas species. The time required to detect all major groups of organisms are the same in both comparisons.

However, the detection of microbial growth in L-BHIB bottle macroscopic examination required sliding unit to be attached and the use of terminal subcultures after 7 days of incubation. The main advantage of L-BHIB biphasic system when compared to either TSB biphasic system or upgraded Isolator system recovery mushrooms.

Nocardia asteroides GUH-2 is not acid-fast when grown in brain heart infusion (BHI) broth. When grown in BHI broth equipped with paraffin, many filamentous cells showed the acid-fastness after treatment with 1% acid-alcohol as a decolorizing agent. When treated with 3% acid-alcohol, the cells not acid-fast filaments. In addition to acid-fast cell filaments of nocardiae, unknown acid-fast round body was observed in the culture broth BHI paraffin-equipped.

Folic acid supplements are important for brain heart infusion broth for the cultivation and cloning of Leishmania donovani promastigotes.

Propagation of virulent and avirulent Leishmania donovani promastigotes inhibited by brain heart infusion broth (BHI) (3.7%) are equipped with haemolysed or whole blood (2.5-10%) or heat-inactivated fetal bovine serum (HIFBS) (10-20 %). Peptone, glutamine, tryptophan, purine bases, ribonucleosides, deoxyribonucleosides, B-vitamins and vitamin C were evaluated for reversal of this growth inhibition.

BHI supplementation with folic acid is further complemented by haemolysed rabbit blood or HIFBS and haemin supported serial propagation in subpassages two primary isolates and 3 more virulent strain of L. donovani and 1 strain of L. major. Folic acid is equipped with a 3% BHI blood of rabbits in solid phase with 0.7% agar colony formation also supports L. donovani. This text documents the development of high efficiency media for cultivation of L. donovani promastigotes in the liquid phase and a colony on agar plates using BHI as a basal medium.

HEART INFUSION BROTH
H08-101-2Kg 2 Kg
EUR 340.8

HEART INFUSION BROTH
H08-101-500g 500 g
EUR 136.8

BRAIN HEART INFUSION BROTH W/O DEXTROSE
B02-114-10kg 10 kg
EUR 1532.4

BRAIN HEART INFUSION BROTH W/O DEXTROSE
B02-114-2kg 2kg
EUR 380.4

BRAIN HEART INFUSION BROTH W/O DEXTROSE
B02-114-500g 500 g
EUR 146.4

Brain Heart Infusion
abx082020-100g 100 g
EUR 276

BRAIN HEART INFUSION AGAR
B02-112-10kg 10 kg
EUR 1496.4

BRAIN HEART INFUSION AGAR
B02-112-2kg 2kg
EUR 372

BRAIN HEART INFUSION AGAR
B02-112-500g 500 g
EUR 145.2

BRAIN HEART INFUSION SOLIDS
B02-129-10kg 10 kg
EUR 1203.6

BRAIN HEART INFUSION SOLIDS
B02-129-2Kg 2 Kg
EUR 308.4

BRAIN HEART INFUSION SOLIDS
B02-129-500g 500 g
EUR 124.8

Bacto Brain Heart Infusion
237500 EACH
EUR 157.32

HEART INFUSION AGAR
H08-100-10kg 10 kg
EUR 1399.2

HEART INFUSION AGAR
H08-100-2kg 2kg
EUR 351.6

HEART INFUSION AGAR
H08-100-500g 500 g
EUR 139.2

BHI Broth w 10% Horse Blood
MED1142 PK50
EUR 48.79

Brain Hrt Infusion 100gm
237400 EACH
EUR 82.08

Heart Inf Broth 500gm
238400 EACH
EUR 225.72

Brain Heart Infus 500gm
211059 EACH
EUR 133.38

Brain Heart Infus 500gm
211065 EACH
EUR 186.96

Brain Heart Inf 2Kg
237200 EACH
EUR 627

BHI Solids
MED1502 EACH
EUR 88.92

BHI & 10% Glycerol
MED1138 PK50
EUR 34.2

POTATO INFUSION AGAR
P16-120-10kg 10 kg
EUR 2798.4

POTATO INFUSION AGAR
P16-120-2kg 2kg
EUR 655.2

POTATO INFUSION AGAR
P16-120-500g 500 g
EUR 222

Cooked Meat-BHI Overlay
MED1036 PK50
EUR 53.92

Hrt Infusion Agar 500gm
244400 EACH
EUR 214.32

LB BROTH - LENNOX LB BROTH
L12-101-10kg 10 kg
EUR 680.4

LB BROTH - LENNOX LB BROTH
L12-101-2kg 2kg
EUR 194.4

LB BROTH - LENNOX LB BROTH
L12-101-500g 500 g
EUR 97.2

LURIA BROTH - MILLER'S LB BROTH
L12-112-10kg 10 kg
EUR 694.8

LURIA BROTH - MILLER'S LB BROTH
L12-112-2kg 2kg
EUR 198

LURIA BROTH - MILLER'S LB BROTH
L12-112-500g 500 g
EUR 97.2

BRUCELLA BROTH
B02-120-10kg 10 kg
EUR 1340.4

BRUCELLA BROTH
B02-120-2Kg 2 Kg
EUR 338.4

BRUCELLA BROTH
B02-120-500g 500 g
EUR 135.6

BOLTON'S BROTH
B02-132-10kg 10 kg
EUR 2122.8

BOLTON'S BROTH
B02-132-2kg 2kg
EUR 508.8

BOLTON'S BROTH
B02-132-500g 500 g
EUR 182.4

Duper Broth
D7000-050 500ml
EUR 262.8

DEXTROSE BROTH
D04-108-10kg 10 kg
EUR 954

DEXTROSE BROTH
D04-108-2kg 2kg
EUR 254.4

DEXTROSE BROTH
D04-108-500g 500 g
EUR 112.8

COLUMBIA BROTH
C03-112-10kg 10 kg
EUR 1372.8

COLUMBIA BROTH
C03-112-2Kg 2 Kg
EUR 345.6

COLUMBIA BROTH
C03-112-500g 500 g
EUR 138

EUGONIC BROTH
E05-107-10kg 10 kg
EUR 1269.6

EUGONIC BROTH
E05-107-2kg 2kg
EUR 322.8

EUGONIC BROTH
E05-107-500g 500 g
EUR 132

EXTRACT BROTH
E05-109-10kg 10 kg
EUR 60

EXTRACT BROTH
E05-109-2kg 2kg
EUR 60

EXTRACT BROTH
E05-109-500g 500 g
EUR 60

M BROTH
M13-100-10kg 10 kg
EUR 1554

M BROTH
M13-100-2kg 2kg
EUR 385.2

M BROTH
M13-100-500g 500 g
EUR 148.8

MACCONKEY BROTH
M13-111-10kg 10 kg
EUR 1196.4

MACCONKEY BROTH
M13-111-2Kg 2 Kg
EUR 307.2

MACCONKEY BROTH
M13-111-500g 500 g
EUR 127.2

NUTRIENT BROTH
N14-103-10kg 10 kg
EUR 988.8

NUTRIENT BROTH
N14-103-2Kg 2 Kg
EUR 261.6

NUTRIENT BROTH
N14-103-500g 500 g
EUR 115.2

NZM BROTH
N14-104-10kg 10 kg
EUR 962.4

NZM BROTH
N14-104-2kg 2kg
EUR 256.8

NZM BROTH
N14-104-500g 500 g
EUR 112.8

NITRATE BROTH
N14-106-10kg 10 kg
EUR 1713.6

NITRATE BROTH
N14-106-2kg 2kg
EUR 420

NITRATE BROTH
N14-106-500g 500 g
EUR 157.2

NZYM BROTH
N14-109-10kg 10 kg
EUR 571.2

NZYM BROTH
N14-109-2Kg 2 Kg
EUR 171.6

NZYM BROTH
N14-109-500g 500 g
EUR 90

NZ BROTH
N14-110-10kg 10 kg
EUR 1306.8

NZ BROTH
N14-110-2kg 2kg
EUR 331.2

NZ BROTH
N14-110-500g 500 g
EUR 133.2

LACTOSE BROTH
L12-105-10kg 10 kg
EUR 931.2

LACTOSE BROTH
L12-105-2kg 2kg
EUR 249.6

LACTOSE BROTH
L12-105-500g 500 g
EUR 111.6

LIM BROTH
L12-129-10kg 10 kg
EUR 944.4

LIM BROTH
L12-129-2Kg 2 Kg
EUR 250.8

LIM BROTH
L12-129-500g 500 g
EUR 111.6

GN BROTH
G07-101-10kg 10 kg
EUR 1293.6

GN BROTH
G07-101-2Kg 2 Kg
EUR 327.6

GN BROTH
G07-101-500g 500 g
EUR 133.2

LB Broth
G246 500 g
EUR 177.6

FRASER BROTH
F06-102-10kg 10 kg
EUR 1004.4

FRASER BROTH
F06-102-2Kg 2 Kg
EUR 265.2

FRASER BROTH
F06-102-500g 500 g
EUR 116.4

PHOTOBACTERIUM BROTH
P16-143-10kg 10 kg Ask for price

PHOTOBACTERIUM BROTH
P16-143-2Kg 2 Kg Ask for price

PHOTOBACTERIUM BROTH
P16-143-500g 500 g Ask for price

HYLB Broth
SD7001 100g
EUR 75.66

SOB Broth
SD7007 250g
EUR 84.54

SOC Broth
SD7009 250g
EUR 86.1

NZCYM Broth
SD7010 100g
EUR 75.59

NZYM Broth
SD7012 100g
EUR 73.06

NZM Broth
SD7014 100g
EUR 73.06

YT Broth
SD7016 250g
EUR 86.1

2xYT Broth
SD7019 250g
EUR 86.1

YPD Broth
SD7022 250g
EUR 86.1

Super Broth
SD7026 250g
EUR 86.1

Lambda Broth
SD7029 100g
EUR 72.53

Terrific Broth
SD7035 250g
EUR 86.1

SCHAEDLER BROTH
S19-105-10kg 10 kg
EUR 1509.6

SCHAEDLER BROTH
S19-105-2Kg 2 Kg
EUR 375.6

SCHAEDLER BROTH
S19-105-500g 500 g
EUR 145.2

SELENITE BROTH
S19-107-10kg 10 kg
EUR 1018.8

SELENITE BROTH
S19-107-2kg 2kg
EUR 268.8

SELENITE BROTH
S19-107-500g 500 g
EUR 116.4

SF BROTH
S19-109-10kg 10 kg
EUR 2197.2

SF BROTH
S19-109-2Kg 2 Kg
EUR 524.4

SF BROTH
S19-109-500g 500 g
EUR 186

SUPER BROTH
S19-128-10kg 10 kg
EUR 1005.6

SUPER BROTH
S19-128-2Kg 2 Kg
EUR 265.2

SUPER BROTH
S19-128-500g 500 g
EUR 116.4

TAT BROTH
T20-100-10kg 10 kg
EUR 1393.2

TAT BROTH
T20-100-2Kg 2 Kg
EUR 349.2

TAT BROTH
T20-100-500g 500 g
EUR 139.2

TRYPTOSE BROTH
T20-116-10kg 10 kg
EUR 1082.4

TRYPTOSE BROTH
T20-116-2kg 2kg
EUR 282

TRYPTOSE BROTH
T20-116-500g 500 g
EUR 120

TPGY BROTH
T20-127-10kg 10 kg
EUR 1663.2

TPGY BROTH
T20-127-2Kg 2 Kg
EUR 408

TPGY BROTH
T20-127-500g 500 g
EUR 154.8

Four Clostridium perfringens strains recovered from clinical specimens, four strains obtained from specimens feces of healthy adults, and the control strain ATCC 13124 was inoculated into Schaedler and Brain Heart Infusion broth, respectively, and incubated anaerobically at 35 degrees C for 24 hours. The exponential growth phase usually lasts from 2 to 6 hours after inoculation; generation times averaging 23 minutes at 2 to 4 hours and 27 minutes between 4 and 6 hours of incubation. The (generation time between 6 and 8 h = 89 minutes) initial growth phase stationary reached at 8 hours after inoculation. All strains shown are essentially identical in vitro growth rate, although they differ in virulence murine.