Clinical comparison of the recoveries of bloodstream pathogens in Septi-Chek brain heart infusion broth with saponin, Septi-Chek tryptic soy broth, and the isolator lysis-centrifugation system.
The recovery of microorganisms in the Septi-Chek biphasic broth tryptic soy (TSB) and Isolator blood culture system as compared to a recovery in Septi-Chek brain biphasic infusion of heart broth is equipped with an agent saponin lytic (L-BHIB; Roche Diagnostics, a Division of Hoffmann-La Roche Inc.). A total of 5,096 TSB-L-BHIB and 1,778 sets of blood culture-L-BHIB Isolator set is evaluated. There are 435 pathogenic organisms recovered in 413 (8.1%) of culture TSB-L-BHIB, with 348 organisms in TSB and 370 bottles in the bottle L-BHIB (P less than 0.05).
Significantly more isolates of yeast found in the system L-BHIB (P less than 0.01). subculture terminal from L-BHIB system is required to detect 13 clinically significant organisms. Significantly more organisms found in a bottle of L-BHIB compared with Isolator system (P less than 0.001), including Torulopsis glabrata, coagulase-negative staphylococci, and Pseudomonas species. The time required to detect all major groups of organisms are the same in both comparisons.
However, the detection of microbial growth in L-BHIB bottle macroscopic examination required sliding unit to be attached and the use of terminal subcultures after 7 days of incubation. The main advantage of L-BHIB biphasic system when compared to either TSB biphasic system or upgraded Isolator system recovery mushrooms.
Nocardia asteroides GUH-2 is not acid-fast when grown in brain heart infusion (BHI) broth. When grown in BHI broth equipped with paraffin, many filamentous cells showed the acid-fastness after treatment with 1% acid-alcohol as a decolorizing agent. When treated with 3% acid-alcohol, the cells not acid-fast filaments. In addition to acid-fast cell filaments of nocardiae, unknown acid-fast round body was observed in the culture broth BHI paraffin-equipped.
Folic acid supplements are important for brain heart infusion broth for the cultivation and cloning of Leishmania donovani promastigotes.
Propagation of virulent and avirulent Leishmania donovani promastigotes inhibited by brain heart infusion broth (BHI) (3.7%) are equipped with haemolysed or whole blood (2.5-10%) or heat-inactivated fetal bovine serum (HIFBS) (10-20 %). Peptone, glutamine, tryptophan, purine bases, ribonucleosides, deoxyribonucleosides, B-vitamins and vitamin C were evaluated for reversal of this growth inhibition.
BHI supplementation with folic acid is further complemented by haemolysed rabbit blood or HIFBS and haemin supported serial propagation in subpassages two primary isolates and 3 more virulent strain of L. donovani and 1 strain of L. major. Folic acid is equipped with a 3% BHI blood of rabbits in solid phase with 0.7% agar colony formation also supports L. donovani. This text documents the development of high efficiency media for cultivation of L. donovani promastigotes in the liquid phase and a colony on agar plates using BHI as a basal medium.
BRAIN HEART INFUSION BROTH |
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B02-113-2Kg | Alphabiosciences | 2 Kg | EUR 366 |
BRAIN HEART INFUSION BROTH |
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B02-113-500g | Alphabiosciences | 500 g | EUR 142.8 |
Brain Heart Infusion Broth |
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HY-157355 | MedChemExpress | Get quote | Ask for price |
Description: Brain Heart Infusion Broth (BHI) is suitable for microbiological culture (For example: Anopheles arabiensis). |
Brain Heart Infusion Broth - EACH |
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MED1434 | Scientific Laboratory Supplies | EACH | EUR 124.2 |
Brain Heart Infusion Broth (Powder) |
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MBS653157-100g | MyBiosource | 100g | EUR 175 |
Brain Heart Infusion Broth (Powder) |
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MBS653157-1kg | MyBiosource | 1kg | EUR 565 |
Brain Heart Infusion Broth (Powder) |
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MBS653157-500g | MyBiosource | 500g | EUR 385 |
Brain Heart Infusion Broth (Powder) |
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MBS653157-5x1kg | MyBiosource | 5x1kg | EUR 2445 |
BRAIN HEART INFUSION BROTH W/O DEXTROSE |
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B02-114-10kg | Alphabiosciences | 10 kg | EUR 1532.4 |
BRAIN HEART INFUSION BROTH W/O DEXTROSE |
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B02-114-2kg | Alphabiosciences | 2kg | EUR 380.4 |
BRAIN HEART INFUSION BROTH W/O DEXTROSE |
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B02-114-500g | Alphabiosciences | 500 g | EUR 146.4 |
Brain Heart Infusion Broth w/o Dextrose (Powder) |
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MBS652861-100g | MyBiosource | 100g | EUR 340 |
Brain Heart Infusion Broth w/o Dextrose (Powder) |
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MBS652861-1kg | MyBiosource | 1kg | EUR 835 |
Brain Heart Infusion Broth w/o Dextrose (Powder) |
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MBS652861-500g | MyBiosource | 500g | EUR 570 |
Brain Heart Infusion Broth w/o Dextrose (Powder) |
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MBS652861-5x1kg | MyBiosource | 5x1kg | EUR 3665 |
Brain Heart Infusion Agar (BHI Agar) |
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94124 | Sisco Laboratories | 100 Gms | EUR 6.84 |
Description: Part D |
Brain Heart Infusion Agar (BHI Agar) |
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94124-1 | Sisco Laboratories | 500 Gms | EUR 27.37 |
Description: Part D |
Brain Heart Infusion Agar (BHI Agar) |
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94124-2 | Sisco Laboratories | 5 Kg | EUR 259.14 |
Description: Part D |
Brain Heart Infusion (BHI)Agar - EACH |
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MED1432 | Scientific Laboratory Supplies | EACH | EUR 113.4 |
HEART INFUSION BROTH |
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H08-101-10kg | Alphabiosciences | 10 kg | EUR 1351.2 |
HEART INFUSION BROTH |
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H08-101-2Kg | Alphabiosciences | 2 Kg | EUR 340.8 |
HEART INFUSION BROTH |
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H08-101-500g | Alphabiosciences | 500 g | EUR 136.8 |
BHI HiVeg™ w/PABA (Brain Heart Infusion |
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MV212-500G | EWC Diagnostics | 1 unit | EUR 45.83 |
Description: BHI HiVeg™ w/PABA (Brain Heart Infusion |
Heart Infusion Broth (HI Broth) |
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75134 | Sisco Laboratories | 100 Gms | EUR 7.7 |
Description: Part D |
Heart Infusion Broth (HI Broth) |
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75134-1 | Sisco Laboratories | 500 Gms | EUR 32.5 |
Description: Part D |
Brain Heart Infusion Agar, Modified (BHI Agar, Modified) |
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82856 | Sisco Laboratories | 100 Gms | EUR 7.7 |
Description: Part D |
Brain Heart Infusion Agar, Modified (BHI Agar, Modified) |
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82856-1 | Sisco Laboratories | 500 Gms | EUR 30.79 |
Description: Part D |
Brain Heart Infusion Agar, Modified (BHI Agar, Modified) |
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82856-2 | Sisco Laboratories | 5 Kg | EUR 280.53 |
Description: Part D |
Heart Infusion Broth (Powder) |
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MBS652800-500g | MyBiosource | 500g | EUR 435 |
Heart Infusion Broth (Powder) |
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MBS652800-5x500g | MyBiosource | 5x500g | EUR 1800 |
Brain Heart Infusion w/ 0.1% Agar (BHI w/ 0.1% Agar) |
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84560 | Sisco Laboratories | 100 Gms | EUR 8.55 |
Description: Part D |
Brain Heart Infusion w/ 0.1% Agar (BHI w/ 0.1% Agar) |
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84560-1 | Sisco Laboratories | 500 Gms | EUR 30.79 |
Description: Part D |
Brain Heart Infusion w/ PABA and Agar (BHI Agar w/PABA) |
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26671 | Sisco Laboratories | 100 Gms | EUR 9.41 |
Description: Part D |
Brain Heart Infusion w/ PABA and Agar (BHI Agar w/PABA) |
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26671-1 | Sisco Laboratories | 500 Gms | EUR 37.63 |
Description: Part D |
Brain Heart Infusion |
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abx082020-100g | Abbexa | 100 g | EUR 276 |
Brain Heart Infusion |
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abx082020-100l | Abbexa | 100 µl | EUR 237.5 |
Brain Heart Infusion |
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abx082020-1ml | Abbexa | 1 ml | Ask for price |
Brain Heart Infusion |
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abx082020-200l | Abbexa | 200 µl | Ask for price |
Brain Heart Infusion Powder (BHI Powder) BactoBio for bacteriology |
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64944 | Sisco Laboratories | 500 Gms | EUR 23.95 |
Description: Part D |
Brain Heart Infusion Powder (BHI Powder) BactoBio for bacteriology |
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64944-1 | Sisco Laboratories | 5 Kg | EUR 224.93 |
Description: Part D |
BRAIN HEART INFUSION AGAR |
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B02-112-10kg | Alphabiosciences | 10 kg | EUR 1496.4 |
BRAIN HEART INFUSION AGAR |
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B02-112-2kg | Alphabiosciences | 2kg | EUR 372 |
BRAIN HEART INFUSION AGAR |
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B02-112-500g | Alphabiosciences | 500 g | EUR 145.2 |
Brain Heart Infusion Agar |
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HY-157375 | MedChemExpress | Get quote | Ask for price |
Description: Brain Heart Infusion Agar is growth medium for the cultivation of a wide variety of microorganisms[1]. |
BRAIN HEART INFUSION SOLIDS |
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B02-129-10kg | Alphabiosciences | 10 kg | EUR 1203.6 |
BRAIN HEART INFUSION SOLIDS |
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B02-129-2Kg | Alphabiosciences | 2 Kg | EUR 308.4 |
BRAIN HEART INFUSION SOLIDS |
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B02-129-500g | Alphabiosciences | 500 g | EUR 124.8 |
Brain-Heart Infusion Broth w/o Dextrose, NaCl (Powder) |
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MBS652857-100g | MyBiosource | 100g | EUR 300 |
Brain-Heart Infusion Broth w/o Dextrose, NaCl (Powder) |
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MBS652857-1kg | MyBiosource | 1kg | EUR 1280 |
Brain-Heart Infusion Broth w/o Dextrose, NaCl (Powder) |
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MBS652857-500g | MyBiosource | 500g | EUR 860 |
Brain-Heart Infusion Broth w/o Dextrose, NaCl (Powder) |
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MBS652857-5x1kg | MyBiosource | 5x1kg | EUR 5670 |
Brain Heart Infusion Agar (Powder) |
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MBS653248-100g | MyBiosource | 100g | EUR 245 |
Brain Heart Infusion Agar (Powder) |
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MBS653248-1kg | MyBiosource | 1kg | EUR 925 |
Brain Heart Infusion Agar (Powder) |
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MBS653248-500g | MyBiosource | 500g | EUR 570 |
Brain Heart Infusion Agar (Powder) |
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MBS653248-5x1kg | MyBiosource | 5x1kg | EUR 4070 |
V Infusion Broth (Veal Infusion Broth) |
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86771 | Sisco Laboratories | 100 Gms | EUR 8.55 |
Description: Part D |
V Infusion Broth (Veal Infusion Broth) |
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86771-1 | Sisco Laboratories | 500 Gms | EUR 30.79 |
Description: Part D |
Liver Infusion Broth (LI Broth) |
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96453 | Sisco Laboratories | 100 Gms | EUR 6.84 |
Description: Part D |
Liver Infusion Broth (LI Broth) |
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96453-1 | Sisco Laboratories | 500 Gms | EUR 24.8 |
Description: Part D |
V Infusion Broth |
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M329-500G | EWC Diagnostics | 1 unit | EUR 43.27 |
Description: V Infusion Broth |
Potato Infusion Broth |
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M1833-500G | EWC Diagnostics | 1 unit | EUR 40.51 |
Description: Potato Infusion Broth |
Infusion Broth (Powder) |
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MBS653130-500g | MyBiosource | 500g | EUR 475 |
Infusion Broth (Powder) |
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MBS653130-5x500g | MyBiosource | 5x500g | EUR 1995 |
HEART INFUSION AGAR |
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H08-100-10kg | Alphabiosciences | 10 kg | EUR 1399.2 |
HEART INFUSION AGAR |
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H08-100-2kg | Alphabiosciences | 2kg | EUR 351.6 |
HEART INFUSION AGAR |
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H08-100-500g | Alphabiosciences | 500 g | EUR 139.2 |
Beef Heart Infusion |
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HY-157374 | MedChemExpress | Get quote | Ask for price |
Description: Beef Heart Infusion is a non-selective microbial culture medium formulation. Beef Heart Infusion provides nutrients for microbial growth. Beef Heart Infusion can be used for the culture, transplantation and rejuvenation of microorganisms. |
Brain-Heart Infusion Solids (Powder) |
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MBS653073-500g | MyBiosource | 500g | EUR 370 |
Brain-Heart Infusion Solids (Powder) |
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MBS653073-5x500g | MyBiosource | 5x500g | EUR 1510 |
Heart Infusion Agar (Powder) |
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MBS653006-500g | MyBiosource | 500g | EUR 415 |
Heart Infusion Agar (Powder) |
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MBS653006-5x500g | MyBiosource | 5x500g | EUR 1710 |
Heart Infusion Agar (HI Agar) |
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47704 | Sisco Laboratories | 100 Gms | EUR 8.55 |
Description: Part D |
Heart Infusion Agar (HI Agar) |
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47704-1 | Sisco Laboratories | 500 Gms | EUR 35.92 |
Description: Part D |
Brain Hrt Infusion 100gm - EACH |
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237400 | Scientific Laboratory Supplies | EACH | EUR 109.35 |
BHI Agar (Special Infusion Agar) |
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M211-100G | EWC Diagnostics | 1 unit | EUR 13.42 |
Description: BHI Agar (Special Infusion Agar) |
BHI Agar (Special Infusion Agar) |
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M211-500G | EWC Diagnostics | 1 unit | EUR 33.1 |
Description: BHI Agar (Special Infusion Agar) |
BHI Agar (Special Infusion Agar), Granul |
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GM211-500G | EWC Diagnostics | 1 unit | EUR 38.09 |
Description: BHI Agar (Special Infusion Agar), Granul |
Heart Infusion Powder (HI Powder) BactoBio for bacteriology |
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28551 | Sisco Laboratories | 500 Gms | EUR 19.67 |
Description: Part D |
Heart Infusion Powder (HI Powder) BactoBio for bacteriology |
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28551-1 | Sisco Laboratories | 2.5 Kg | EUR 88.09 |
Description: Part D |
BHI HiCynth™ Agar ( Special Infusion HiC |
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MCD211-100G | EWC Diagnostics | 1 unit | EUR 15.46 |
Description: BHI HiCynth™ Agar ( Special Infusion HiC |
BHI HiCynth™ Agar ( Special Infusion HiC |
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MCD211-500G | EWC Diagnostics | 1 unit | EUR 38.09 |
Description: BHI HiCynth™ Agar ( Special Infusion HiC |
Brain Heart Infus 500gm |
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211065 | Scientific Laboratory Supplies | EACH | EUR 201.78 |
Brain Heart Infus 500gm - EACH |
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211059 | Scientific Laboratory Supplies | EACH | EUR 178.2 |
V Infusion Agar (Veal Infusion Agar) |
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78604 | Sisco Laboratories | 100 Gms | EUR 11.12 |
Description: Part D |
V Infusion Agar (Veal Infusion Agar) |
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78604-1 | Sisco Laboratories | 500 Gms | EUR 33.36 |
Description: Part D |
Potato Infusion |
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41600005-1 | Glycomatrix | 100 g | EUR 25.12 |
Potato Infusion |
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41600005-2 | Glycomatrix | 500 g | EUR 83.08 |
Potato Infusion |
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41600005-3 | Glycomatrix | 1 kg | EUR 153.37 |
V Infusion Agar |
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M328-500G | EWC Diagnostics | 1 unit | EUR 46.08 |
Description: V Infusion Agar |
LM Infusion Agar |
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M1206-500G | EWC Diagnostics | 1 unit | EUR 42.8 |
Description: LM Infusion Agar |
HiVeg™ Infusion |
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RM191V-500G | EWC Diagnostics | 1 unit | EUR 49.58 |
Description: HiVeg™ Infusion |
HL Infusion Powder |
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RM022-500G | EWC Diagnostics | 1 unit | EUR 34.17 |
Description: HL Infusion Powder |
HM Infusion Powder |
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RM192-500G | EWC Diagnostics | 1 unit | EUR 34.52 |
Description: HM Infusion Powder |
Potato Infusion Agar |
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M174-500G | EWC Diagnostics | 1 unit | EUR 38.38 |
Description: Potato Infusion Agar |
POTATO INFUSION AGAR |
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P16-120-10kg | Alphabiosciences | 10 kg | EUR 2798.4 |
POTATO INFUSION AGAR |
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P16-120-2kg | Alphabiosciences | 2kg | EUR 655.2 |
POTATO INFUSION AGAR |
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P16-120-500g | Alphabiosciences | 500 g | EUR 222 |
Standard Infusion Agar |
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M883-500G | EWC Diagnostics | 1 unit | EUR 42.8 |
Description: Standard Infusion Agar |
HiVeg# Special Infusion |
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RM188V-500G | EWC Diagnostics | 1 unit | EUR 38.25 |
Description: HiVeg# Special Infusion |
LM Infusion HiVeg Agar |
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MV1206-500G | EWC Diagnostics | 1 unit | EUR 42.8 |
Description: LM Infusion HiVeg Agar |
HiVeg™ Infusion No. 1 |
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RM022V-500G | EWC Diagnostics | 1 unit | EUR 35.37 |
Description: HiVeg™ Infusion No. 1 |
HiVeg™ Infusion No. 2 |
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RM192V-500G | EWC Diagnostics | 1 unit | EUR 35.48 |
Description: HiVeg™ Infusion No. 2 |
Potato Infusion HiVeg Agar |
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MV174-500G | EWC Diagnostics | 1 unit | EUR 38.38 |
Description: Potato Infusion HiVeg Agar |
Standard Infusion Agar, HiVeg |
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MV883-500G | EWC Diagnostics | 1 unit | EUR 42.8 |
Description: Standard Infusion Agar, HiVeg |
Hrt Infusion Agar 500gm - EACH |
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244400 | Scientific Laboratory Supplies | EACH | EUR 284.85 |
Liver Infusion Agar (LI Agar) |
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68861 | Sisco Laboratories | 100 Gms | EUR 7.7 |
Description: Part D |
Liver Infusion Agar (LI Agar) |
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68861-1 | Sisco Laboratories | 500 Gms | EUR 29.93 |
Description: Part D |
Blood Agar Base (Infusion Agar) |
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M073-100G | EWC Diagnostics | 1 unit | EUR 13.42 |
Description: Blood Agar Base (Infusion Agar) |
Blood Agar Base (Infusion Agar) |
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M073-500G | EWC Diagnostics | 1 unit | EUR 33.1 |
Description: Blood Agar Base (Infusion Agar) |
Blood Agar Base (Infusion Agar) |
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GM073-100G | EWC Diagnostics | 1 unit | EUR 12.97 |
Description: Blood Agar Base (Infusion Agar) |
Blood Agar Base (Infusion Agar) |
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GM073-500G | EWC Diagnostics | 1 unit | EUR 38.09 |
Description: Blood Agar Base (Infusion Agar) |
VEAL INFUSION VIRAL TRANSPORT MEDIUM |
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7574002 | Lampire Biologicals Laboratories | 1ML | EUR 59.77 |
Viral Transport Medium Veal Infusion |
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IGVTMV1000ML | Innovative research | each | EUR 141 |
Description: Viral Transport Medium Veal Infusion |
Viral Transport Medium Veal Infusion |
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IGVTMV500ML | Innovative research | each | EUR 81 |
Description: Viral Transport Medium Veal Infusion |
Viral Transport Medium Veal Infusion |
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MBS136574-1L | MyBiosource | 1L | EUR 265 |
Viral Transport Medium Veal Infusion |
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MBS136574-500mL | MyBiosource | 500mL | EUR 200 |
Viral Transport Medium Veal Infusion |
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MBS136574-5x1L | MyBiosource | 5x1L | EUR 1055 |
Blood Agar Base, HiVeg (Infusion Agar, |
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MV073-100G | EWC Diagnostics | 1 unit | EUR 13.42 |
Description: Blood Agar Base, HiVeg (Infusion Agar, |
Blood Agar Base, HiVeg (Infusion Agar, |
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MV073-500G | EWC Diagnostics | 1 unit | EUR 33.1 |
Description: Blood Agar Base, HiVeg (Infusion Agar, |
Blood HiCynth Agar Base (Infusion HiCynt |
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MCD073-100G | EWC Diagnostics | 1 unit | EUR 15.46 |
Description: Blood HiCynth Agar Base (Infusion HiCynt |
Blood HiCynth Agar Base (Infusion HiCynt |
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MCD073-500G | EWC Diagnostics | 1 unit | EUR 38.09 |
Description: Blood HiCynth Agar Base (Infusion HiCynt |
VEAL INFUSION VIRAL TRANSPORT MEDIUM, 1LTR |
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7574002-1LTR | Lampire Biologicals Laboratories | 1LTR | EUR 111.02 |
VEAL INFUSION VIRAL TRANSPORT MEDIUM, 1 ML |
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7574002-A | Lampire Biologicals Laboratories | 1ML | EUR 14.39 |
BHI Broth |
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M210-100G | EWC Diagnostics | 1 unit | EUR 13.42 |
Description: BHI Broth |
Four Clostridium perfringens strains recovered from clinical specimens, four strains obtained from specimens feces of healthy adults, and the control strain ATCC 13124 was inoculated into Schaedler and Brain Heart Infusion broth, respectively, and incubated anaerobically at 35 degrees C for 24 hours. The exponential growth phase usually lasts from 2 to 6 hours after inoculation; generation times averaging 23 minutes at 2 to 4 hours and 27 minutes between 4 and 6 hours of incubation. The (generation time between 6 and 8 h = 89 minutes) initial growth phase stationary reached at 8 hours after inoculation. All strains shown are essentially identical in vitro growth rate, although they differ in virulence murine.