In a comparison of 1,368 positive blood culture, vented Roche Septi-Chek (V-RSC) blood culture bottles is superior to the tryptic soy broth containing bottles of unvented (Difco) for the recovery of all the aerobic and facultative anaerobic microorganisms. Anaerobic bacteria were recovered more often and earlier in unvented tryptic soy broth containing bottle. A separate comparison of 529 positive blood culture bottles was conducted to test the performance of the V-RSC with biphasic media vented from the brain heart infusion. Bottle V-RSC recovered significantly more isolates of Enterobacteriaceae and anaerobic bacteria than brain heart infusion medium vented biphasic. Bottle V-RSC is a reliable blood culture system for all aerobic and facultative anaerobic microorganisms. Due to its suboptimal recovery of anaerobic bacteria, it is recommended that a bottle of V-RSC is used in combination with a vacuum blood culture bottles unvented.
Panmede, papain digest cow’s liver, added to brain heart infusion broth cysteine non-sporing increase anaerobic recovery from simulated blood cultures in which human blood is added to each broth. Brain heart infusion broth containing cysteine and backed Panmede best isolation of non-sporing first anaerobic incubation for 48 hours compared to four commercial media are tested in parallel. good recovery during the first 24 hours of anaerobic obtained with heart brain infusion broth equipped with cysteine and Panmede, chatty anaerobic thioglycollate broth and Brewer of the Southern Group of Thiol Laboratories but not other types of intermediate and Brewer thioglycollate media tested.
Penicillin-like drugs were found unstable in Schaedler broth in storage’S forzen (–20 degrees C). Chloramphenicol, clindamycin and tetracycline remain in the original potential for 45 days. There is no detectable antimicrobial decay was found in two formulations of include BHI broth. The antimicrobial potency is measured by the quality control organisms endpoint, bioassay and MIC changes were used in four clinical microbiology laboratory.
Short-term incubation of positive blood cultures in brain-heart infusion broth accelerate the identification of bacteria by matrix-assisted laser desorption / ionization time-of-flight mass spectrometry.
OBJECTIVE
rapid identification of bacteria directly from positive blood culture (BC) with matrix-assisted laser desorption / ionization time-of-flight mass spectrometry (MALDI-TOF MS) can be achieved either using MALDI Sepsityper kit (method of protein extraction) or after a period of short-step pre-cultivation on solid media. We developed a new method that involves short-term enrichment positive BCs in brain-heart infusion broth (BHI) before the MALDI-TOF MS analysis.
METHOD
Eighty-four BCs characterized as positive included in this study; are processed in parallel either directly using MALDI Sepsityper kit or follow short-term culture both in BHI or Columbia blood agar with 5% sheep blood (CBA).
RESULTS
bacterial species were identified in 91.6, 89.2 and 65.4% of cases after pre-cultivated for 4 hours in BHI, the CBA, or by using the MALDI Sepsityper kit, respectively. Overall, the average incubation time for the correct identification shorter when pre-culture performed in BHI; the mean time to Gram-negative rods in BHI was 78.2 minutes and 108.2 minutes in the CBA (P = 0.045), and the average time for Gram-positive cocci was 128.5 minutes and 169.6 minutes in BHI at CBA (P = 0.013).
 19 mm Diameter Solid Titanium Tip |
|
0-120-0013 |
Biologics |
19 mm |
EUR 570 |
 25 mm Diameter Tapped Titanium Tip |
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0-120-0014 |
Biologics |
25 mm |
EUR 682 |
 25 mm Diameter Solid Titanium Tip |
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0-120-0015 |
Biologics |
25 mm |
EUR 614 |
 Flat Replaceable Tips 13 mm Diameter Flat Titanium Tip |
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0-120-0016 |
Biologics |
13 mm |
EUR 86 |
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Description: use with corresponding Tapped Tips and Tapped Extender Tips |
 Flat Replaceable Tips 19 mm Diameter Flat Titanium Tip |
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0-120-0017 |
Biologics |
19 mm |
EUR 92 |
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Description: use with corresponding Tapped Tips and Tapped Extender Tips |
 Flat Replaceable Tips 25 mm Diameter Flat Titanium Tip |
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0-120-0018 |
Biologics |
25 mm |
EUR 100 |
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Description: use with corresponding Tapped Tips and Tapped Extender Tips |
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0-120-0019 |
Biologics |
250 ml |
EUR 1377 |
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Description: includes Interface Washers |
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0-120-0021 |
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each |
EUR 113 |
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Description: includes Interface Washers |
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each |
EUR 774 |
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1.5'' Diameter |
EUR 31 |
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13 mm |
EUR 301 |
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Biologics |
13 mm |
EUR 362 |
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0-120-0034 |
Biologics |
19 mm |
EUR 315 |
 19 mm Diameter Tapped Titanium Extender Tip |
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0-120-0035 |
Biologics |
19 mm |
EUR 375 |
 25 mm Diameter Solid Titanium Extender Tip |
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0-120-0036 |
Biologics |
25 mm |
EUR 328 |
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0-120-0037 |
Biologics |
25 mm |
EUR 389 |
 KoldPod, 1.5ml Micro Tube |
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0-120-0038 |
Biologics |
1.5 ml |
EUR 152 |
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Description: Thermo conductive tube pods |
 KoldPod, 15ml Conical Tube |
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0-120-0039 |
Biologics |
15 ml |
EUR 275 |
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Description: Thermo conductive tube pods |
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0-120-0040 |
Biologics |
50 ml |
EUR 290 |
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Description: Thermo conductive tube pods |
 Model 150 V/T Ultrasonic Homogenizer |
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0-121-0002 |
Biologics |
210-240V/50-60Hz |
EUR 2920 |
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Description: Delivers up to 150 Watts of ultrasonic power to the Titanium Tip. The Timer and Duty Cycle function increase preciosion in sample processing processing. |
 Model 300 V/T Ultrasonic Homogenizer |
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0-122-0002 |
Biologics |
210-240V/50-60Hz |
EUR 3520 |
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Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip. The Timer and Duty Cycle function increase preciosion in sample. |
 SONABOZ Sound Abating Chamber |
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0-125-0001 |
Biologics |
each |
EUR 1020 |
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Description: Reduces cavitational sound emitted during processing. |
 Model 3000 Ultrasonic Homogenizer |
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0-127-0002 |
Biologics |
210-240V/50-60Hz |
EUR 4120 |
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Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip and includes an intergrated Sound Abating Chmaber to reduce cavitational sound emitted during processing. The Timer and Duty Cycle function increase preciosion in sample. |
 Model 3000MP Ultrasonic Homogenizer |
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0-128-0002 |
Biologics |
210-240V/50-60Hz |
EUR 4720 |
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Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip with preciosion control from a microprocessor and a graphical user interface displayed on a large (145 mm) LCD display. The integrated Sound Abating Chamber reduces cavitational sound emitted during processing. |
 OMNICON® Zone Reader, 210-240V/50-60Hz |
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0-131-0002 |
Biologics |
210-240V/50-60Hz |
EUR 35200 |
|
|
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Description: Designed to Perform multi-plate Assays on round 90/100mm Petri Dishes. The integrated LED illumination system provides transmitted light for brightfield and darkfield illumination of transparent media. |
 OMNI-Noculator Peni Cylinder Filler, 210-240V/50-60Hz |
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0-134-0002 |
Biologics |
210-240V/50-60Hz |
EUR 32200 |
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Description: A robotic liquid handling system designed to dispense Peni Cylinders and fill Peni Cylinders with the corresponding antibiotic liquid sample. |
 Peni Cylinder Dispenser with Manual Hopper |
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0-144-0002 |
Biologics |
each |
EUR 5406 |
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Description: Dispenser can be configured to dispense 4 or 6 Peni Cylinders onto a petri dish. |
 Peni Cylinder Dispenser with Motorized Hopper, 100-240V/50-60Hz |
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0-144-0003 |
Biologics |
100-240V/50-60Hz |
EUR 6254 |
|
Description: The motorized hopper can be configured to dispense 4 or 6 Peni Cylinders onto a petri Dispenser can be disassembled for disinfection. |
 Stainless Steel Peni Cylinder with Flat Face |
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0-144-0005 |
Biologics |
6mm I.D. x 8mm O.D. x 10mm Long |
EUR 399 |
|
Description: sold in packages of 100 pieces |
 Stainless Steel Peni Cylinder with Chamfered Face |
|
0-144-0006 |
Biologics |
6mm I.D. x 8mm O.D. x 10mm Long |
EUR 412 |
|
Description: sold in packages of 100 pieces |
) Custom development of ELISAs for other species or antibody isotypes not listed in the catalog. Custom testing of samples for IgG/IgM/IgA or total (IgG+IgM+IgA) |
|
000-CUS |
Alpha Diagnostics |
Custom |
EUR 602 |
 Alpha-Bungarotoxin, CF®405S, 500 ug |
|
00002 |
Biotium |
1UG |
EUR 527 |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
 Alpha-bungarotoxin, CF405s |
|
00002 |
Cusabio |
500uG |
EUR 594 |
|
Description: Minimum order quantity: 1 unit of 500uG |
Alpha-Bungarotoxin, CF®405S, 500 ug |
|
00002-1 |
Biotium |
EA |
EUR 527 |
 Alpha-Bungarotoxin, CF®405S 100ug |
|
00002-100ug |
Biotium |
100uG |
EUR 132 |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
 Alpha-Bungarotoxin, CF®680R, 500 ug |
|
9-00003 |
Biotium |
-
Ask for price
-
Ask for price
|
|
|
|
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Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
Alpha-Bungarotoxin, CF®680R, 500 ug |
|
00003-1 |
Biotium |
EA |
EUR 527 |
 Alpha-Bungarotoxin, CF®680R 100ug |
|
9-00003 |
Biotium |
-
Ask for price
-
Ask for price
|
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|
|
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Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
 Alpha-Bungarotoxin, CF®640R, 500 ug |
|
9-00004 |
Biotium |
-
Ask for price
-
Ask for price
|
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Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
Alpha-Bungarotoxin, CF®640R, 500 ug |
|
00004-1 |
Biotium |
EA |
EUR 527 |
 Alpha-Bungarotoxin, CF®640R 100ug |
|
9-00004 |
Biotium |
-
Ask for price
-
Ask for price
|
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|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
 Alpha-Bungarotoxin, CF®488A, 500 ug |
|
9-00005 |
Biotium |
-
Ask for price
-
Ask for price
|
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|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
Alpha-Bungarotoxin, CF®488A, 500 ug |
|
00005-1 |
Biotium |
EA |
EUR 527 |
 Alpha-Bungarotoxin CF®488A 100ug |
|
9-00005 |
Biotium |
-
Ask for price
-
Ask for price
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Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
 Alpha-Bungarotoxin, CF®568, 500 ug |
|
9-00006 |
Biotium |
-
Ask for price
-
Ask for price
|
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|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
Alpha-Bungarotoxin, CF®568, 500 ug |
|
00006-1 |
Biotium |
EA |
EUR 527 |
 Alpha-Bungarotoxin, CF®568 100ug |
|
9-00006 |
Biotium |
-
Ask for price
-
Ask for price
|
|
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
 Alpha-Bungarotoxin, CF®594, 500 ug |
|
9-00007 |
Biotium |
-
Ask for price
-
Ask for price
|
|
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
Alpha-Bungarotoxin, CF®594, 500 ug |
|
00007-1 |
Biotium |
EA |
EUR 527 |
 Alpha-Bungarotoxin CF®594 100ug |
|
9-00007 |
Biotium |
-
Ask for price
-
Ask for price
|
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|
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|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
 Alpha-Bungarotoxin, CF®633, 500 ug |
|
9-00009 |
Biotium |
-
Ask for price
-
Ask for price
|
|
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
Alpha-Bungarotoxin, CF®633, 500 ug |
|
00009-1 |
Biotium |
EA |
EUR 527 |
 Alpha-Bungarotoxin, CF®633 100ug |
|
9-00009 |
Biotium |
-
Ask for price
-
Ask for price
|
|
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
 SARS-CoV-2 Indicator Cell Line for RNA Replication - GFP Reporter only |
|
0001-PP-001 |
IBT Bioservices |
1 cell line (can order x amount) |
EUR 12000 |
|
Description: SARS-CoV-2 GFP reporter cell line using HEK293T (ACE2/TMPRSS2) cells |
 Alpha-Bungarotoxin, 1 mg |
|
00010-1 |
Biotium |
1MG |
EUR 193 |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
Alpha-Bungarotoxin, 1 mg |
|
00010-1-1 |
Biotium |
EA |
EUR 193 |
 Fluorescein-Alpha-Bungarotoxin, 500 ug |
|
00011 |
Biotium |
500uG |
EUR 376 |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
Fluorescein-Alpha-Bungarotoxin, 500 ug |
|
00011-1 |
Biotium |
EA |
EUR 376 |
 Tetramethylrhodamine-Alpha-Bungarotoxin, 500 ug |
|
00012 |
Biotium |
500uG |
EUR 394 |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
Tetramethylrhodamine-Alpha-Bungarotoxin, 500 ug |
|
00012-1 |
Biotium |
EA |
EUR 394 |
 Fluorescein-alpha-bungarotoxin, 10x50ug |
|
00013 |
Biotium |
10ST |
EUR 436 |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
Fluorescein-alpha-bungarotoxin, 10x50ug |
|
00013-1 |
Biotium |
EA |
EUR 436 |
 Tetramethylrhodamine-A-Bungarotoxin, 10x50 ug |
|
00014 |
Biotium |
10ST |
EUR 494 |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
Tetramethylrhodamine-A-Bungarotoxin, 10x50 ug |
|
00014-1 |
Biotium |
EA |
EUR 494 |
 Sulforhodamine 101-Alpha-Bungarotoxin, 500 ug |
|
00015 |
Biotium |
500uG |
EUR 494 |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
Sulforhodamine 101-Alpha-Bungarotoxin, 500 ug |
|
00015-1 |
Biotium |
EA |
EUR 494 |
 Sulforhodamine 101-Alpha-Bungarotoxin, 50 ug |
|
00016 |
Biotium |
10ST |
EUR 560 |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
Sulforhodamine 101-Alpha-Bungarotoxin, 50 ug |
|
00016-1 |
Biotium |
EA |
EUR 560 |
 Biotin-XX-A-Bungarotoxin, 500 ug |
|
00017 |
Biotium |
500uG |
EUR 455 |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
Biotin-XX-A-Bungarotoxin, 500 ug |
|
00017-1 |
Biotium |
EA |
EUR 455 |
 Alpha-Bungarotoxin, CF®555, 500 ug |
|
9-00018 |
Biotium |
-
Ask for price
-
Ask for price
|
|
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
Alpha-Bungarotoxin, CF®555, 500 ug |
|
00018-1 |
Biotium |
EA |
EUR 527 |
 Alpha-Bungarotoxin, CF®555 100ug |
|
9-00018 |
Biotium |
-
Ask for price
-
Ask for price
|
|
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
 Biotin-cAMP, 1 mg |
|
00020 |
Biotium |
1MG |
EUR 298 |
|
|
|
Description: N/A |
Biotin-cAMP, 1 mg |
|
00020-1 |
Biotium |
20ST |
EUR 298 |
|
|
|
Description: N/A |
 Biotin-cAMP, 50 ug |
|
00020-1-1 |
Biotium |
EA |
EUR 414 |
 Biotin-cGMP, 1 mg |
|
00021 |
Biotium |
1MG |
EUR 331 |
|
|
|
Description: N/A |
Biotin-cGMP, 1 mg |
|
00021-1 |
Biotium |
20ST |
EUR 331 |
|
|
|
Description: N/A |
 Biotin-cGMP, 20x50 ug |
|
00021-1-1 |
Biotium |
EA |
EUR 447 |
 Cyanine 644-cAMP, 1 mg |
|
00022 |
Biotium |
1MG |
EUR 496 |
|
|
|
Description: N/A |
Cyanine 644-cAMP, 1 mg |
|
00022-1 |
Biotium |
20ST |
EUR 496 |
|
|
|
Description: N/A |
 Cyanine 644-cAMP, 20x50 ug |
|
00022-1-1 |
Biotium |
EA |
EUR 647 |
 Fluorescein Methotrexate, Triammonium Salt, 1 mg |
|
00023 |
Biotium |
1MG |
EUR 285 |
|
|
|
Description: N/A |
Fluorescein Methotrexate, Triammonium Salt, 1 mg |
|
00023-1 |
Biotium |
EA |
EUR 285 |
 Staurosporine |
|
00025 |
Biotium |
100uG |
EUR 100 |
|
|
|
Description: N/A |
Staurosporine |
|
00025-1 |
Biotium |
EA |
EUR 100 |
 Alpha-Bungarotoxin, CF®543, 500 ug |
|
00026 |
Biotium |
500uG |
EUR 527 |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
Alpha-Bungarotoxin, CF®543, 500 ug |
|
00026-1 |
Biotium |
EA |
EUR 527 |
 Alpha-Bungarotoxin, CF®543, 100 ug |
|
00026-100ug |
Biotium |
1UG |
EUR 132 |
|
|
|
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus) |
 Rhodamine Phalloidin 300U |
|
00027 |
Biotium |
300 |
EUR 364 |
|
|
|
Description: N/A |
Rhodamine Phalloidin 300U |
|
00027-1 |
Biotium |
EA |
EUR 364 |
 Biotin-XX-Phalloidin |
|
00028 |
Biotium |
100U |
EUR 466 |
|
|
|
Description: N/A |
Biotin-XX-Phalloidin |
|
00028-1 |
Biotium |
EA |
EUR 466 |
 Fluorescein-Phalloidin |
|
00030 |
Biotium |
300U |
EUR 364 |
|
|
|
Description: N/A |
Fluorescein-Phalloidin |
|
00030-1 |
Biotium |
EA |
EUR 364 |
 Rhodamine 110 Phalloidin |
|
00032 |
Biotium |
300ST |
EUR 364 |
|
|
|
Description: N/A |
Rhodamine 110 Phalloidin |
|
00032-1 |
Biotium |
EA |
EUR 364 |
 Phalloidin) Sulforhodamine 101 (Texas Red®) Phalloidin |
|
00033 |
Biotium |
300EU |
EUR 364 |
|
|
|
Description: N/A |
Sulforhodamine 101 (Texas Red®) Phalloidin |
|
00033-1 |
Biotium |
EA |
EUR 364 |
 Phalloidin, CF®405M |
|
00034 |
Biotium |
300U |
EUR 482 |
|
|
|
Description: N/A |
Phalloidin, CF®405M |
|
00034-1 |
Biotium |
EA |
EUR 482 |
Phalloidin, CF®405M |
|
00034-T |
Biotium |
50U |
EUR 101 |
|
|
|
Description: N/A |
Phalloidin, CF®405M |
|
00034-T-1 |
Biotium |
EA |
EUR 101 |
 CF®488A-cAMP |
|
00036 |
Biotium |
100ug |
EUR 136 |
|
|
|
Description: N/A |
CF®488A-cAMP |
|
00036-1 |
Biotium |
EA |
EUR 136 |
 CF®640R-cAMP |
|
00037 |
Biotium |
100ug |
EUR 136 |
|
|
|
Description: N/A |
CF®640R-cAMP |
|
00037-1 |
Biotium |
EA |
EUR 136 |
 Phalloidin, CF555 |
|
00040 |
Biotium |
300U |
EUR 530.4 |
|
Description: Minimum order quantity: 1 unit of 300U |
Phalloidin, CF555 |
|
00040-T |
Biotium |
50U |
EUR 159.6 |
|
Description: Minimum order quantity: 1 unit of 50U |
 Phalloidin, CF®647, 300 U |
|
00041 |
Biotium |
300U |
EUR 482 |
|
|
|
Description: N/A |
Phalloidin, CF®647, 300 U |
|
00041-1 |
Biotium |
EA |
EUR 482 |
 Phalloidin, CF®647, 50 U |
|
00041-T |
Biotium |
50U |
EUR 101 |
|
|
|
Description: N/A |
Phalloidin, CF®647, 50 U |
|
00041-T-1 |
Biotium |
EA |
EUR 101 |
 Phalloidin, CF®488A, 300 U |
|
00042 |
Biotium |
300U |
EUR 482 |
|
|
|
Description: N/A |
Phalloidin, CF®488A, 300 U |
|
00042-1 |
Biotium |
EA |
EUR 482 |
 Phalloidin, CF®488A, 50 U |
|
00042-T |
Biotium |
50U |
EUR 101 |
|
|
|
Description: N/A |
Phalloidin, CF®488A, 50 U |
|
00042-T-1 |
Biotium |
EA |
EUR 101 |
 Phalloidin, CF®543, 300 U |
|
00043 |
Biotium |
300U |
EUR 482 |
|
|
|
Description: N/A |
Phalloidin, CF®543, 300 U |
|
00043-1 |
Biotium |
EA |
EUR 482 |
 Phalloidin, CF®543, 50 U |
|
00043-T |
Biotium |
50U |
EUR 101 |
|
|
|
Description: N/A |
Phalloidin, CF®543, 50 U |
|
00043-T-1 |
Biotium |
EA |
EUR 101 |
 Phalloidin, CF®568, 300 U |
|
00044 |
Biotium |
300U |
EUR 482 |
|
|
|
Description: N/A |
Phalloidin, CF®568, 300 U |
|
00044-1 |
Biotium |
EA |
EUR 482 |
CONCLUSION
Short-term enrichment of BCs in BHI accelerate the identification of a number of species of bacteria by MALDI-TOF MS. Further prospective study is needed to validate our method and measure the potential clinical impact in the management of bacterial infection in the bloodstream.
