In a comparison of 1,368 positive blood culture, vented Roche Septi-Chek (V-RSC) blood culture bottles is superior to the tryptic soy broth containing bottles of unvented (Difco) for the recovery of all the aerobic and facultative anaerobic microorganisms. Anaerobic bacteria were recovered more often and earlier in unvented tryptic soy broth containing bottle. A separate comparison of 529 positive blood culture bottles was conducted to test the performance of the V-RSC with biphasic media vented from the brain heart infusion. Bottle V-RSC recovered significantly more isolates of Enterobacteriaceae and anaerobic bacteria than brain heart infusion medium vented biphasic. Bottle V-RSC is a reliable blood culture system for all aerobic and facultative anaerobic microorganisms. Due to its suboptimal recovery of anaerobic bacteria, it is recommended that a bottle of V-RSC is used in combination with a vacuum blood culture bottles unvented.

Panmede, papain digest cow’s liver, added to brain heart infusion broth cysteine ​​non-sporing increase anaerobic recovery from simulated blood cultures in which human blood is added to each broth. Brain heart infusion broth containing cysteine ​​and backed Panmede best isolation of non-sporing first anaerobic incubation for 48 hours compared to four commercial media are tested in parallel. good recovery during the first 24 hours of anaerobic obtained with heart brain infusion broth equipped with cysteine ​​and Panmede, chatty anaerobic thioglycollate broth and Brewer of the Southern Group of Thiol Laboratories but not other types of intermediate and Brewer thioglycollate media tested.

Penicillin-like drugs were found unstable in Schaedler broth in storage’S forzen (–20 degrees C). Chloramphenicol, clindamycin and tetracycline remain in the original potential for 45 days. There is no detectable antimicrobial decay was found in two formulations of include BHI broth. The antimicrobial potency is measured by the quality control organisms endpoint, bioassay and MIC changes were used in four clinical microbiology laboratory.

Short-term incubation of positive blood cultures in brain-heart infusion broth accelerate the identification of bacteria by matrix-assisted laser desorption / ionization time-of-flight mass spectrometry.

OBJECTIVE
rapid identification of bacteria directly from positive blood culture (BC) with matrix-assisted laser desorption / ionization time-of-flight mass spectrometry (MALDI-TOF MS) can be achieved either using MALDI Sepsityper kit (method of protein extraction) or after a period of short-step pre-cultivation on solid media. We developed a new method that involves short-term enrichment positive BCs in brain-heart infusion broth (BHI) before the MALDI-TOF MS analysis.

METHOD
Eighty-four BCs characterized as positive included in this study; are processed in parallel either directly using MALDI Sepsityper kit or follow short-term culture both in BHI or Columbia blood agar with 5% sheep blood (CBA).

RESULTS
bacterial species were identified in 91.6, 89.2 and 65.4% of cases after pre-cultivated for 4 hours in BHI, the CBA, or by using the MALDI Sepsityper kit, respectively. Overall, the average incubation time for the correct identification shorter when pre-culture performed in BHI; the mean time to Gram-negative rods in BHI was 78.2 minutes and 108.2 minutes in the CBA (P = 0.045), and the average time for Gram-positive cocci was 128.5 minutes and 169.6 minutes in BHI at CBA (P = 0.013).

Flat Replaceable Tips 19 mm Diameter Flat Titanium Tip
0-120-0017	Biologics	19 mm	EUR 92
Description: use with corresponding Tapped Tips and Tapped Extender Tips

Flat Replaceable Tips 25 mm Diameter Flat Titanium Tip
0-120-0018	Biologics	25 mm	EUR 100
Description: use with corresponding Tapped Tips and Tapped Extender Tips

Titanium Cup Tip, 250 ml
0-120-0019	Biologics	250 ml	EUR 1377
Description: includes Interface Washers

Microtube Tray, 8 Position (for 250 ml Cup Tip)
0-120-0021	Biologics	each	EUR 113
Description: includes Interface Washers

Continuous Flow Chamber
0-120-0026	Biologics	each	EUR 774
Description: includes Interface Washers

Interface Washers 1.5” Diameter, 5/Pkg
0-120-003	Biologics	1.5'' Diameter	EUR 31

13 mm Diameter Solid Titanium Extender Tip
0-120-0032	Biologics	13 mm	EUR 301

13 mm Diameter Tapped Titanium Extender Tip
0-120-0033	Biologics	13 mm	EUR 362

19 mm Diameter Solid Titanium Extender Tip
0-120-0034	Biologics	19 mm	EUR 315

19 mm Diameter Tapped Titanium Extender Tip
0-120-0035	Biologics	19 mm	EUR 375

25 mm Diameter Solid Titanium Extender Tip
0-120-0036	Biologics	25 mm	EUR 328

25 mm Diameter Tapped Titanium Extender Tip
0-120-0037	Biologics	25 mm	EUR 389

KoldPod, 1.5ml Micro Tube
0-120-0038	Biologics	1.5 ml	EUR 152
Description: Thermo conductive tube pods

KoldPod, 15ml Conical Tube
0-120-0039	Biologics	15 ml	EUR 275
Description: Thermo conductive tube pods

KoldPod, 50ml Conical Tube
0-120-0040	Biologics	50 ml	EUR 290
Description: Thermo conductive tube pods

Model 150 V/T Ultrasonic Homogenizer
0-121-0002	Biologics	210-240V/50-60Hz	EUR 2920
Description: Delivers up to 150 Watts of ultrasonic power to the Titanium Tip. The Timer and Duty Cycle function increase preciosion in sample processing processing.

Model 300 V/T Ultrasonic Homogenizer
0-122-0002	Biologics	210-240V/50-60Hz	EUR 3520
Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip. The Timer and Duty Cycle function increase preciosion in sample.

SONABOZ Sound Abating Chamber
0-125-0001	Biologics	each	EUR 1020
Description: Reduces cavitational sound emitted during processing.

Model 3000 Ultrasonic Homogenizer
0-127-0002	Biologics	210-240V/50-60Hz	EUR 4120
Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip and includes an intergrated Sound Abating Chmaber to reduce cavitational sound emitted during processing. The Timer and Duty Cycle function increase preciosion in sample.

Model 3000MP Ultrasonic Homogenizer
0-128-0002	Biologics	210-240V/50-60Hz	EUR 4720
Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip with preciosion control from a microprocessor and a graphical user interface displayed on a large (145 mm) LCD display. The integrated Sound Abating Chamber reduces cavitational sound emitted during processing.

OMNICON® Zone Reader, 210-240V/50-60Hz
0-131-0002	Biologics	210-240V/50-60Hz	EUR 35200
Description: Designed to Perform multi-plate Assays on round 90/100mm Petri Dishes. The integrated LED illumination system provides transmitted light for brightfield and darkfield illumination of transparent media.

OMNI-Noculator Peni Cylinder Filler, 210-240V/50-60Hz
0-134-0002	Biologics	210-240V/50-60Hz	EUR 32200
Description: A robotic liquid handling system designed to dispense Peni Cylinders and fill Peni Cylinders with the corresponding antibiotic liquid sample.

Peni Cylinder Dispenser with Manual Hopper
0-144-0002	Biologics	each	EUR 5406
Description: Dispenser can be configured to dispense 4 or 6 Peni Cylinders onto a petri dish.

Peni Cylinder Dispenser with Motorized Hopper, 100-240V/50-60Hz
0-144-0003	Biologics	100-240V/50-60Hz	EUR 6254
Description: The motorized hopper can be configured to dispense 4 or 6 Peni Cylinders onto a petri Dispenser can be disassembled for disinfection.

Stainless Steel Peni Cylinder with Flat Face
0-144-0005	Biologics	6mm I.D. x 8mm O.D. x 10mm Long	EUR 399
Description: sold in packages of 100 pieces

Stainless Steel Peni Cylinder with Chamfered Face
0-144-0006	Biologics	6mm I.D. x 8mm O.D. x 10mm Long	EUR 412
Description: sold in packages of 100 pieces

Custom development of ELISAs for other species or antibody isotypes not listed in the catalog. Custom testing of samples for IgG/IgM/IgA or total (IgG+IgM+IgA)
000-CUS	Alpha Diagnostics	Custom	EUR 602

Alpha-Bungarotoxin, CF®405S, 500 ug
00002	Biotium	1UG	EUR 527
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-bungarotoxin, CF405s
00002	Cusabio	500uG	EUR 594
Description: Minimum order quantity: 1 unit of 500uG

Alpha-Bungarotoxin, CF®405S, 500 ug
00002-1	Biotium	EA	EUR 527

Alpha-Bungarotoxin, CF®405S 100ug
00002-100ug	Biotium	100uG	EUR 132
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®680R, 500 ug
9-00003	Biotium	Ask for price Ask for price	500uG 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®680R, 500 ug
00003-1	Biotium	EA	EUR 527

Alpha-Bungarotoxin, CF®680R 100ug
9-00003	Biotium	Ask for price Ask for price	500uG 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®640R, 500 ug
9-00004	Biotium	Ask for price Ask for price	500uG 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®640R, 500 ug
00004-1	Biotium	EA	EUR 527

Alpha-Bungarotoxin, CF®640R 100ug
9-00004	Biotium	Ask for price Ask for price	500uG 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®488A, 500 ug
9-00005	Biotium	Ask for price Ask for price	500uG 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®488A, 500 ug
00005-1	Biotium	EA	EUR 527

Alpha-Bungarotoxin CF®488A 100ug
9-00005	Biotium	Ask for price Ask for price	500uG 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®568, 500 ug
9-00006	Biotium	Ask for price Ask for price	500uG 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®568, 500 ug
00006-1	Biotium	EA	EUR 527

Alpha-Bungarotoxin, CF®568 100ug
9-00006	Biotium	Ask for price Ask for price	500uG 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®594, 500 ug
9-00007	Biotium	Ask for price Ask for price	500uG 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®594, 500 ug
00007-1	Biotium	EA	EUR 527

Alpha-Bungarotoxin CF®594 100ug
9-00007	Biotium	Ask for price Ask for price	500uG 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®633, 500 ug
9-00009	Biotium	Ask for price Ask for price	500uG 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®633, 500 ug
00009-1	Biotium	EA	EUR 527

Alpha-Bungarotoxin, CF®633 100ug
9-00009	Biotium	Ask for price Ask for price	500uG 100uG
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

SARS-CoV-2 Indicator Cell Line for RNA Replication - GFP Reporter only
0001-PP-001	IBT Bioservices	1 cell line (can order x amount)	EUR 12000
Description: SARS-CoV-2 GFP reporter cell line using HEK293T (ACE2/TMPRSS2) cells

Alpha-Bungarotoxin, 1 mg
00010-1	Biotium	1MG	EUR 193
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, 1 mg
00010-1-1	Biotium	EA	EUR 193

Fluorescein-Alpha-Bungarotoxin, 500 ug
00011	Biotium	500uG	EUR 376
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Fluorescein-Alpha-Bungarotoxin, 500 ug
00011-1	Biotium	EA	EUR 376

Tetramethylrhodamine-Alpha-Bungarotoxin, 500 ug
00012	Biotium	500uG	EUR 394
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Tetramethylrhodamine-Alpha-Bungarotoxin, 500 ug
00012-1	Biotium	EA	EUR 394

Fluorescein-alpha-bungarotoxin, 10x50ug
00013	Biotium	10ST	EUR 436
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Fluorescein-alpha-bungarotoxin, 10x50ug
00013-1	Biotium	EA	EUR 436

Tetramethylrhodamine-A-Bungarotoxin, 10x50 ug
00014	Biotium	10ST	EUR 494
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Tetramethylrhodamine-A-Bungarotoxin, 10x50 ug
00014-1	Biotium	EA	EUR 494

Sulforhodamine 101-Alpha-Bungarotoxin, 500 ug
00015	Biotium	500uG	EUR 494
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Sulforhodamine 101-Alpha-Bungarotoxin, 500 ug
00015-1	Biotium	EA	EUR 494

Sulforhodamine 101-Alpha-Bungarotoxin, 50 ug
00016	Biotium	10ST	EUR 560
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Sulforhodamine 101-Alpha-Bungarotoxin, 50 ug
00016-1	Biotium	EA	EUR 560

Biotin-XX-A-Bungarotoxin, 500 ug
00017	Biotium	500uG	EUR 455
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Biotin-XX-A-Bungarotoxin, 500 ug
00017-1	Biotium	EA	EUR 455

Alpha-Bungarotoxin, CF®555, 500 ug
9-00018	Biotium	Ask for price Ask for price	500uG 100ug
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®555, 500 ug
00018-1	Biotium	EA	EUR 527

Alpha-Bungarotoxin, CF®555 100ug
9-00018	Biotium	Ask for price Ask for price	500uG 100ug
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Acrylamide, Chemzymes Ultra Pure®
00019-100	Polysciences Europe GmbH	100g	EUR 101.52
Description: 79-06-1

Acrylamide, Chemzymes Ultra Pure®
00019-500	Polysciences Europe GmbH	500g	EUR 270
Description: 79-06-1

Biotin-cAMP, 1 mg
00020	Biotium	1MG	EUR 298
Description: N/A

Biotin-cAMP, 1 mg
00020-1	Biotium	20ST	EUR 298
Description: N/A

Biotin-cAMP, 50 ug
00020-1-1	Biotium	EA	EUR 414

Biotin-cGMP, 1 mg
00021	Biotium	1MG	EUR 331
Description: N/A

Biotin-cGMP, 1 mg
00021-1	Biotium	20ST	EUR 331
Description: N/A

Biotin-cGMP, 20x50 ug
00021-1-1	Biotium	EA	EUR 447

Cyanine 644-cAMP, 1 mg
00022	Biotium	1MG	EUR 496
Description: N/A

Cyanine 644-cAMP, 1 mg
00022-1	Biotium	20ST	EUR 496
Description: N/A

Cyanine 644-cAMP, 20x50 ug
00022-1-1	Biotium	EA	EUR 647

Fluorescein Methotrexate, Triammonium Salt, 1 mg
00023	Biotium	1MG	EUR 285
Description: N/A

Fluorescein Methotrexate, Triammonium Salt, 1 mg
00023-1	Biotium	EA	EUR 285

Staurosporine
00025	Biotium	100uG	EUR 100
Description: N/A

Staurosporine
00025-1	Biotium	EA	EUR 100

Alpha-Bungarotoxin, CF®543, 500 ug
00026	Biotium	500uG	EUR 527
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Alpha-Bungarotoxin, CF®543, 500 ug
00026-1	Biotium	EA	EUR 527

Alpha-Bungarotoxin, CF®543, 100 ug
00026-100ug	Biotium	1UG	EUR 132
Description: Alpha-bungarotoxin from Krait snake venom (Bungarus multicinctus)

Rhodamine Phalloidin 300U
00027	Biotium	300	EUR 364
Description: N/A

Rhodamine Phalloidin 300U
00027-1	Biotium	EA	EUR 364

Biotin-XX-Phalloidin
00028	Biotium	100U	EUR 466
Description: N/A

Biotin-XX-Phalloidin
00028-1	Biotium	EA	EUR 466

Fluorescein-Phalloidin
00030	Biotium	300U	EUR 364
Description: N/A

Fluorescein-Phalloidin
00030-1	Biotium	EA	EUR 364

Rhodamine 110 Phalloidin
00032	Biotium	300ST	EUR 364
Description: N/A

Rhodamine 110 Phalloidin
00032-1	Biotium	EA	EUR 364

Sulforhodamine 101 (Texas Red®) Phalloidin
00033	Biotium	300EU	EUR 364
Description: N/A

Sulforhodamine 101 (Texas Red®) Phalloidin
00033-1	Biotium	EA	EUR 364

Phalloidin, CF®405M
00034	Biotium	300U	EUR 482
Description: N/A

Phalloidin, CF®405M
00034-1	Biotium	EA	EUR 482

Phalloidin, CF®405M
00034-T	Biotium	50U	EUR 101
Description: N/A

Phalloidin, CF®405M
00034-T-1	Biotium	EA	EUR 101

CF®488A-cAMP
00036	Biotium	100ug	EUR 136
Description: N/A

CF®488A-cAMP
00036-1	Biotium	EA	EUR 136

CF®640R-cAMP
00037	Biotium	100ug	EUR 136
Description: N/A

CF®640R-cAMP
00037-1	Biotium	EA	EUR 136

Phalloidin, CF555
00040	Biotium	300U	EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF555
00040-T	Biotium	50U	EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF®647, 300 U
00041	Biotium	300U	EUR 482
Description: N/A

Phalloidin, CF®647, 300 U
00041-1	Biotium	EA	EUR 482

Phalloidin, CF®647, 50 U
00041-T	Biotium	50U	EUR 101
Description: N/A

Phalloidin, CF®647, 50 U
00041-T-1	Biotium	EA	EUR 101

Phalloidin, CF®488A, 300 U
00042	Biotium	300U	EUR 482
Description: N/A

Phalloidin, CF®488A, 300 U
00042-1	Biotium	EA	EUR 482

Phalloidin, CF®488A, 50 U
00042-T	Biotium	50U	EUR 101
Description: N/A

Phalloidin, CF®488A, 50 U
00042-T-1	Biotium	EA	EUR 101

Phalloidin, CF®543, 300 U
00043	Biotium	300U	EUR 482
Description: N/A

Phalloidin, CF®543, 300 U
00043-1	Biotium	EA	EUR 482

Phalloidin, CF®543, 50 U
00043-T	Biotium	50U	EUR 101
Description: N/A

Phalloidin, CF®543, 50 U
00043-T-1	Biotium	EA	EUR 101

Phalloidin, CF®568, 300 U
00044	Biotium	300U	EUR 482
Description: N/A

Phalloidin, CF®568, 300 U
00044-1	Biotium	EA	EUR 482

Phalloidin, CF®568, 50 U
00044-T	Biotium	50U	EUR 101
Description: N/A

Phalloidin, CF®568, 50 U
00044-T-1	Biotium	EA	EUR 101

Phalloidin, CF®594, 300 U
00045	Biotium	300U	EUR 482
Description: N/A

Phalloidin, CF®594, 300 U
00045-1	Biotium	EA	EUR 482

CONCLUSION
Short-term enrichment of BCs in BHI accelerate the identification of a number of species of bacteria by MALDI-TOF MS. Further prospective study is needed to validate our method and measure the potential clinical impact in the management of bacterial infection in the bloodstream.