Comparison of the Roche Septi-Chek blood culture bottle with a brain heart infusion biphasic medium bottle and with a tryptic soy broth bottle.

In a comparison of 1,368 positive blood culture, vented Roche Septi-Chek (V-RSC) blood culture bottles is superior to the tryptic soy broth containing bottles of unvented (Difco) for the recovery of all the aerobic and facultative anaerobic microorganisms. Anaerobic bacteria were recovered more often and earlier in unvented tryptic soy broth containing bottle. A separate comparison of 529 positive blood culture bottles was conducted to test the performance of the V-RSC with biphasic media vented from the brain heart infusion. Bottle V-RSC recovered significantly more isolates of Enterobacteriaceae and anaerobic bacteria than brain heart infusion medium vented biphasic. Bottle V-RSC is a reliable blood culture system for all aerobic and facultative anaerobic microorganisms. Due to its suboptimal recovery of anaerobic bacteria, it is recommended that a bottle of V-RSC is used in combination with a vacuum blood culture bottles unvented.

Panmede, papain digest cow’s liver, added to brain heart infusion broth cysteine ​​non-sporing increase anaerobic recovery from simulated blood cultures in which human blood is added to each broth. Brain heart infusion broth containing cysteine ​​and backed Panmede best isolation of non-sporing first anaerobic incubation for 48 hours compared to four commercial media are tested in parallel. good recovery during the first 24 hours of anaerobic obtained with heart brain infusion broth equipped with cysteine ​​and Panmede, chatty anaerobic thioglycollate broth and Brewer of the Southern Group of Thiol Laboratories but not other types of intermediate and Brewer thioglycollate media tested.

Penicillin-like drugs were found unstable in Schaedler broth in storage’S forzen (–20 degrees C). Chloramphenicol, clindamycin and tetracycline remain in the original potential for 45 days. There is no detectable antimicrobial decay was found in two formulations of include BHI broth. The antimicrobial potency is measured by the quality control organisms endpoint, bioassay and MIC changes were used in four clinical microbiology laboratory.

Short-term incubation of positive blood cultures in brain-heart infusion broth accelerate the identification of bacteria by matrix-assisted laser desorption / ionization time-of-flight mass spectrometry.

OBJECTIVE
rapid identification of bacteria directly from positive blood culture (BC) with matrix-assisted laser desorption / ionization time-of-flight mass spectrometry (MALDI-TOF MS) can be achieved either using MALDI Sepsityper kit (method of protein extraction) or after a period of short-step pre-cultivation on solid media. We developed a new method that involves short-term enrichment positive BCs in brain-heart infusion broth (BHI) before the MALDI-TOF MS analysis.

METHOD
Eighty-four BCs characterized as positive included in this study; are processed in parallel either directly using MALDI Sepsityper kit or follow short-term culture both in BHI or Columbia blood agar with 5% sheep blood (CBA).

RESULTS
bacterial species were identified in 91.6, 89.2 and 65.4% of cases after pre-cultivated for 4 hours in BHI, the CBA, or by using the MALDI Sepsityper kit, respectively. Overall, the average incubation time for the correct identification shorter when pre-culture performed in BHI; the mean time to Gram-negative rods in BHI was 78.2 minutes and 108.2 minutes in the CBA (P = 0.045), and the average time for Gram-positive cocci was 128.5 minutes and 169.6 minutes in BHI at CBA (P = 0.013).

Alpha-bungarotoxin CF488a

9-00005
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  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF568

9-00006
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  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF568

9-00006
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  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF594

9-00007
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  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin CF594

9-00007
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  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF633

9-00009
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  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF633

9-00009
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  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin

00010-1 1MG
EUR 201.6
Description: Minimum order quantity: 1 unit of 1MG

Fluorescein-Alpha-bungarotoxin

00011 500uG
EUR 440.4
Description: Minimum order quantity: 1 unit of 500uG

Tetramethylrhodamine-Alpha-bungarotoxin

00012 500uG
EUR 459.6
Description: Minimum order quantity: 1 unit of 500uG

Fluorescein-alpha-bungarotoxin: (10x50ug)

00013 10ST
EUR 500.4
Description: Minimum order quantity: 1 unit of 10ST

Tetramethylrhodamine-alpha-bungarotoxin: (10x50ug)

00014 10ST
EUR 559.2
Description: Minimum order quantity: 1 unit of 10ST

Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (500ug)

00015 500uG
EUR 559.2
Description: Minimum order quantity: 1 unit of 500uG

Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (10x50ug)

00016 10ST
EUR 627.6
Description: Minimum order quantity: 1 unit of 10ST

Biotin-xx-a-bungarotoxin

00017 500uG
EUR 519.6
Description: Minimum order quantity: 1 unit of 500uG

Alpha-Bungarotoxin, CF555 conjugate

9-00018
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  • 500uG
  • 100ug
Description: Minimum order quantity: 1 unit of 500uG

Alpha-Bungarotoxin, CF555 conjugate

9-00018
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  • 500uG
  • 100ug
Description: Minimum order quantity: 1 unit of 100ug

Biotin-cAMP, diisopropylethylammonium salt:

00020 1MG
EUR 376.8
Description: Minimum order quantity: 1 unit of 1MG

Biotin-cAMP, diisopropylethylammonium salt: (20x50ug)

00020-1 20ST
EUR 501.6
Description: Minimum order quantity: 1 unit of 20ST

Biotin-cGMP, diisopropylethylammonium salt:

00021 1MG
EUR 412.8
Description: Minimum order quantity: 1 unit of 1MG

Biotin-cGMP, diisopropylethylammonium salt: (20x50ug)

00021-1 20ST
EUR 537.6
Description: Minimum order quantity: 1 unit of 20ST

Cyanine 644-cAMP:

00022 1MG
EUR 589.2
Description: Minimum order quantity: 1 unit of 1MG

Cyanine 644-cAMP: (20x50ug)

00022-1 20ST
EUR 750
Description: Minimum order quantity: 1 unit of 20ST

Fluorescein Methotrexate, triammonium salt:

00023 1MG
EUR 337.2
Description: Minimum order quantity: 1 unit of 1MG

Staurosporine

00025 100uG
EUR 166.8
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF543

00026 500uG
EUR 592.8
Description: Minimum order quantity: 1 unit of 500uG

Rhodamine phalloidin 300u

00027 300
EUR 414
Description: Minimum order quantity: 1 unit of 300

Biotin-xx-phalloidin

00028 100U
EUR 513.6
Description: Minimum order quantity: 1 unit of 100U

Fluorescein-phalloidin

00030 300U
EUR 414
Description: Minimum order quantity: 1 unit of 300U

Rhodamine 110 phalloidin

00032 300ST
EUR 414
Description: Minimum order quantity: 1 unit of 300ST

Sulforhodamine 101 (Texas-Red) Phalloidin

00033 300EU
EUR 414
Description: Minimum order quantity: 1 unit of 300EU

Phalloidin, CF405M

00034 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF405M

00034-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

CF488A-cAMP

00036 100ug
EUR 206.4
Description: Minimum order quantity: 1 unit of 100ug

CF640R-cAMP

00037 100ug
EUR 206.4
Description: Minimum order quantity: 1 unit of 100ug

Phalloidin, CF555

00040 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF555

00040-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF647

00041 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF647

00041-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF488A

00042 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF488A

00042-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF543

00043 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF543

00043-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF568

00044 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF568

00044-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF594

00045 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF594

00045-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF633

00046 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF633

00046-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF660R

00047 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF660R

00047-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF680R

00048 300U
EUR 559.2
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF680R

00048-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF350

00049 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF350

00049-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF640R

00050 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF640R

00050-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF532

00051 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF532

00051-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF660C

00052 300U
EUR 530.4
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF660C

00052-T 50U
EUR 159.6
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF680

00053 300U
EUR 559.2
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF680

00053-T 50U
EUR 176.4
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF430

00054 300U
EUR 538.8
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF430

00054-T 50U
EUR 166.8
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF440

00055 300U
EUR 538.8
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF440

00055-T 50U
EUR 166.8
Description: Minimum order quantity: 1 unit of 50U

Cholera Toxin Subunit B, CF488A conjugate

00070 100ug
EUR 313.2
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF568 conjugate

00071 100ug
EUR 313.2
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF594 conjugate

00072 100ug
EUR 313.2
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF640R conjugate

00073 100ug
EUR 316.8
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF532 conjugate

00074 100ug
EUR 316.8
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF543 conjugate

00075 100ug
EUR 316.8
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF620R conjugate

00076 100ug
EUR 316.8
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF633 conjugate

00077 100ug
EUR 316.8
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF660R conjugate

00078 100ug
EUR 316.8
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF680R conjugate

00079 100ug
EUR 338.4
Description: Minimum order quantity: 1 unit of 100ug

Human Transferrin, CF488A conjugate

00081 1mg
EUR 178.8
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF543 conjugate

00082 1mg
EUR 178.8
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF568 conjugate

00083 1mg
EUR 178.8
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF594 conjugate

00084 1mg
EUR 178.8
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF640R conjugate

00085 1mg
EUR 178.8
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF680R conjugate

00086 1mg
EUR 178.8
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF750 conjugate

00087 1mg
EUR 186
Description: Minimum order quantity: 1 unit of 1mg

HIV-1 tat recombinant antigen

00110-V-01mg 0,1 mg
EUR 321
Description: HIV-1 tat recombinant antigen full length 15 kDa

HIV-1 tat recombinant antigen

00110-V-1000ug 1000 ug
EUR 1539
Description: HIV-1 tat recombinant antigen full length 15 kDa

HIV-1 GAG P24 Recombinant Antigen

00111-V-01mg 0,1 mg
EUR 321
Description: HIV-1 gag p24 recombinant antigen a.a 77 to a.a 436 of the HIV-1 gag region 39 kDa

HIV-1 GAG P24 Recombinant Antigen

00111-V-1000ug 1000 ug
EUR 1539
Description: HIV-1 gag p24 recombinant antigen a.a 77 to a.a 436 of the HIV-1 gag region 39 kDa

HIV-1 nef recombinant antigen

00112-V-01mg 0,1 mg
EUR 321
Description: HIV-1 nef recombinant antigen a.a 3 to a.a 190 of the HIV-1 nef region 20 kDa

HIV-1 nef recombinant antigen

00112-V-1000ug 1000 ug
EUR 1539
Description: HIV-1 nef recombinant antigen a.a 3 to a.a 190 of the HIV-1 nef region 20 kDa

HIV-1 env gp41 recombinant antigen

00113-V-01mg 0,1 mg
EUR 321
Description: HIV-1 env gp41 recombinant antigen a.a 466 to a.a 753 of the HIV-1 env region 32 kDa

HIV-1 env gp41 recombinant antigen

00113-V-1000ug 1000 ug
EUR 1539
Description: HIV-1 env gp41 recombinant antigen a.a 466 to a.a 753 of the HIV-1 env region 32 kDa

HIV-2 GP 36 Recombinant Antigen

00114-V-01mg 0,1 mg
EUR 321
Description: HIV -2 env gp36 recombinant antigen a.a 390 to a.a 702 of the HIV-2 env region 34 kDa

HIV-2 GP 36 Recombinant Antigen

00114-V-1000ug 1000 ug
EUR 1539
Description: HIV -2 env gp36 recombinant antigen a.a 390 to a.a 702 of the HIV-2 env region 34 kDa

HCV core recombinant antigen

00115-V-01mg 0,1 mg
EUR 321
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa

HCV core recombinant antigen

00115-V-1000ug 1000 ug
EUR 1539
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa

HCV core recombinant antigen, Biotin conjugate

00115-V-B-01mg 0,1 mg
EUR 321
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa. Biotin conjugate.

HCV core recombinant antigen, Biotin conjugate

00115-V-B-1000ug 1000 ug
EUR 1539
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa. Biotin conjugate.

HCV core recombinant antigen, FITC conjugate

00115-V-F-01mg 0,1 mg
EUR 321
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa. Fluorescein conjugate.

HCV core recombinant antigen, FITC conjugate

00115-V-F-1000ug 1000 ug
EUR 1539
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa. Fluorescein conjugate.

HCV core recombinant antigen, Rhodamine

00115-V-R-01mg 0,1 mg
EUR 321
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa . Rhodamine conjugate.

HCV core recombinant antigen, Rhodamine

00115-V-R-1000ug 1000 ug
EUR 1539
Description: HCV core recombinant antigen a.a 2 to a.a 192 of HCV polyprotein 22 kDa . Rhodamine conjugate.

HCV NS4 recombinant antigen NS4a+b

00116-V-01mg 0,1 mg
EUR 321
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa.

HCV NS4 recombinant antigen NS4a+b

00116-V-1000ug 1000 ug
EUR 1539
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa.

HCV NS4 recombinant antigen NS4a+b, Biotin

00116-V-B-01mg 0,1 mg
EUR 321
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Biotin conjugate.

HCV NS4 recombinant antigen NS4a+b, Biotin

00116-V-B-1000ug 1000 ug
EUR 1539
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Biotin conjugate.

HCV NS4 recombinant antigen NS4a+b, FITC

00116-V-F-01mg 0,1 mg
EUR 321
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Fluorescein conjugate.

HCV NS4 recombinant antigen NS4a+b, FITC

00116-V-F-1000ug 1000 ug
EUR 1539
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Fluorescein conjugate.

HCV NS4 recombinant antigen NS4a+b, Rhodamine

00116-V-R-01mg 0,1 mg
EUR 321
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Rhodamine conjugate.

HCV NS4 recombinant antigen NS4a+b, Rhodamine

00116-V-R-1000ug 1000 ug
EUR 1539
Description: HCV NS4 recombinant antigen NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa. Rhodamine conjugate.

HCV NS3 recombinant antigen

00117-V-01mg 0,1 mg
EUR 321
Description: HCV NS3 recombinant antigen a.a 1400 to a.a 1643 of HCV polyprotein 22 kDa

HCV NS3 recombinant antigen

00117-V-1000ug 1000 ug
EUR 1539
Description: HCV NS3 recombinant antigen a.a 1400 to a.a 1643 of HCV polyprotein 22 kDa

HBV core recombinant antigen

00120-V-01mg 0,1 mg
EUR 321
Description: HBV core recombinant antigen HBcAg a.a 1 to a.a 183 of HBV core antigen 18 kDa

HBV core recombinant antigen

00120-V-1000ug 1000 ug
EUR 1539
Description: HBV core recombinant antigen HBcAg a.a 1 to a.a 183 of HBV core antigen 18 kDa

HBV core recombinant antigen, Delta

00121-V-01mg 0,1 mg
EUR 321
Description: HBV core recombinant antigen HBcAg a.a 1 to a.a 144 of HBV core antigen 16 kDa

HBV core recombinant antigen, Delta

00121-V-1000ug 1000 ug
EUR 1539
Description: HBV core recombinant antigen HBcAg a.a 1 to a.a 144 of HBV core antigen 16 kDa

HBV surface recombinant antigen HBsAg

00122-V-01mg 0,1 mg
EUR 321
Description: HBV surface recombinant antigen HBsAg antigen 22 kDa Pichia pastoris recombinant

HBV surface recombinant antigen HBsAg

00122-V-1000ug 1000 ug
EUR 1539
Description: HBV surface recombinant antigen HBsAg antigen 22 kDa Pichia pastoris recombinant

HBV surface recombinant antigen HBsAg antigen

00123-V-01mg 0,1 mg
EUR 321
Description: HBV surface recombinant antigen HBsAg antigen 22 kDa Pichia pastoris recombinant High purity

HBV surface recombinant antigen HBsAg antigen

00123-V-1000ug 1000 ug
EUR 1539
Description: HBV surface recombinant antigen HBsAg antigen 22 kDa Pichia pastoris recombinant High purity

HBV surface recombinant antigen HBsAg antigen

00124-V-01mg 0,1 mg
EUR 321
Description: HBV surface recombinant antigen HBsAg antigen 31 kDa E. coli recombinant

HBV surface recombinant antigen HBsAg antigen

00124-V-1000ug 1000 ug
EUR 1539
Description: HBV surface recombinant antigen HBsAg antigen 31 kDa E. coli recombinant

HEV (Birma) ORF2 recombinant antigen

00131-V-01mg 0,1 mg
EUR 321
Description: HEV (Birma) ORF2 recombinant antigen a.a. 633 to a.a 659.

HEV (Birma) ORF2 recombinant antigen

00131-V-1000ug 1000 ug
EUR 1539
Description: HEV (Birma) ORF2 recombinant antigen a.a. 633 to a.a 659.

HEV (Birma) ORF2 recombinant antigen

00132-V-01mg 0,1 mg
EUR 321
Description: HEV (Birma) ORF2 recombinant antigen a.a. 403 to a.a 461.

CONCLUSION
Short-term enrichment of BCs in BHI accelerate the identification of a number of species of bacteria by MALDI-TOF MS. Further prospective study is needed to validate our method and measure the potential clinical impact in the management of bacterial infection in the bloodstream.