BACKGROUND
The purpose of this study was to characterize the growth of Campylobacter in broth enrichment (Bolton broth, brain heart infusion broth), material cecum (in vitro), and in broiler live naturally infected (in vivo) in terms of lag period the mean and time generation and The maximum growth rate and population (cell concentration) is reached.
METHOD
Bolton and brain heart infusion broth and recovered materials cecum poultry inoculated with 10 strains of Campylobacter (eight Campylobacter jejuni and Campylobacter coli two), incubated in microaerobic conditions, and the concentration of Campylobacter determined periodically using ISO 10 272: 2006. Methods caeca than 10 flock, which infected in the first thinning, is used to characterize the growth of Campylobacter in live birds. mean generation time (G) (initial lag phase to exponential) is calculated using the formula: G = t / 3.3 logb / B. Mean time lag and μmax calculated using Micro Fit (©) Software (Version 1.0, the Institute of Food Research). Statistical comparison is done by using GENSTAT ver. 14.1 (VSN International Ltd., Hemel Hempstead, UK).
RESULTS
Lag period meant Bolton broth, brain heart infusion broth, cecum material, and the live bird was estimated to be 6.6, 6.7, 12.6, and 31.3 hours, respectively. The corresponding mean generation times of 2.1, 2.2, 3.1, and 6.7 hours, respectively; The maximum growth rate was 0.7, 0.8, 0.4, and 2 generations h (-1) and the maximum population obtained in each matrix were 9.6, 9.9, 7.8, and 7.4 log10 CFU / g, respectively.
CONCLUSION
This study provides data on the growth of Campylobacter in various media lab, the contents of the cecum, and broiler can be used to develop predictive models and / or inform the control strategy based on science as the maximum time between testing herd and slaughter, slaughter logistics, and one stage depopulation unit broiler.
Analysis of 11 years of microbial keratitis in the South West of England using a brain-heart infusion broth.
The purpose of this study was to identify the organisms responsible for microbial keratitis, as identified by the friction of the cornea using a brain-heart infusion broth, trends over time and antimicrobial susceptibility, over a period of 11 years in the two units of the South West of England; Bristol Eye Hospital and the Royal United Hospital, corneal scratches Bath.All done and sent for microbiological analysis between 4 April 2006 and October 31, 2017 in the two units of the retrospective review.
The first line treatment is monotherapy with levofloxacin 0.5% and second-line treatment is a combination of cefuroxime 5% and 1.5% gentamicin. Both direct and enriching culture used.In total, 2614 scratch the cornea of 2116 patients (1082 women, average age 47.7 ± 21.2 years) were identified. 38.1% (n = 996) were positive cultures and organism cultured in 1195. In all, 91.6% is bacteria (69.4% were gram-positive, gram negative 30.6%).
 human Resistin ELISA KIT |
|
201-12-0339 |
SunredBio |
96 tests |
EUR 528 |
|
|
|
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
 Human Resistin ELISA kit |
|
55R-1714 |
Fitzgerald |
1 kit |
EUR 805.2 |
|
Description: ELISA kit for the detection of Human Resistin in the research laboratory |
Human Resistin ELISA kit |
|
55R-2127 |
Fitzgerald |
96 tests |
EUR 919.2 |
|
Description: ELISA Kit for detection of Resistin in the research laboratory |
Human Resistin ELISA kit |
|
55R-E50 |
Fitzgerald |
96 wells |
EUR 1413.6 |
|
Description: ELISA kit for the detection of Human Resistin in the research laboratory |
human Resistin ELISA KIT |
|
CN-04165H1 |
ChemNorm |
96T |
EUR 530.4 |
human Resistin ELISA KIT |
|
CN-04165H2 |
ChemNorm |
48T |
EUR 351.6 |
 Human Resistin ELISA KIT |
|
CSB-E06884h-24T |
Cusabio |
1 plate of 24 wells |
EUR 198 |
|
|
|
Description: Quantitativesandwich ELISA kit for measuring Human Resistin in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
Human Resistin ELISA KIT |
|
1-CSB-E06884h |
Cusabio |
-
EUR 722.40
-
EUR 5085.60
-
EUR 2707.20
|
- 1 plate of 96 wells
- 10 plates of 96 wells each
- 5 plates of 96 wells each
|
|
|
|
Description: Quantitativesandwich ELISA kit for measuring Human Resistin in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
Human Resistin ELISA kit |
|
E01R0351-192T |
BlueGene |
192 tests |
EUR 1524 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Human Resistin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Resistin ELISA kit |
|
E01R0351-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Human Resistin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Resistin ELISA kit |
|
E01R0351-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
|
|
Description: A competitive ELISA for quantitative measurement of Human Resistin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Resistin ELISA kit |
|
LF-EK50441 |
Abfrontier |
1×96T |
EUR 790.8 |
 Human Resistin ELISA Kit |
|
LF-EK70021 |
Abfrontier |
1×96T |
EUR 625.2 |
Human Resistin ELISA Kit |
|
EHR0358 |
Abclonal |
96Tests |
EUR 625.2 |
Human Resistin ELISA Kit |
|
RK00139 |
Abclonal |
96 Tests |
EUR 625.2 |
Human Resistin ELISA Kit |
|
E28L0045 |
EnoGene |
96T |
EUR 666.67 |
Human Resistin ELISA KIT |
|
E42EH-93 |
EnoGene |
96T/48T |
Ask for price |
Human Resistin ELISA Kit |
|
E16HE0035 |
EnoGene |
96T |
EUR 833.33 |
Human Resistin ELISA KIT |
|
E16HE0035-048 |
EnoGene |
48 wells |
EUR 295 |
Human Resistin ELISA KIT |
|
E16HE0035-096 |
EnoGene |
96 wells |
EUR 395 |
Human Resistin ELISA kit |
|
E22-HC136.48 |
EnoGene |
48T |
EUR 295 |
Human Resistin ELISA kit |
|
E22-HC136.96 |
EnoGene |
96T |
EUR 395 |
Human Resistin ELISA Kit |
|
SL1530Hu |
Sunlong |
96 Tests |
EUR 468 |
|
|
 Resistin, human recombinant |
|
4559-100 |
Biovision |
each |
EUR 874.8 |
Resistin, human recombinant |
|
4559-1000 |
Biovision |
each |
EUR 6127.2 |
Resistin, human recombinant |
|
4559-25 |
Biovision |
each |
EUR 307.2 |
 Protein) Human Resistin (RETN) Protein |
|
20-abx068871 |
Abbexa |
-
EUR 777.60
-
EUR 326.40
-
EUR 2331.60
-
EUR 910.80
-
EUR 560.40
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
|
Human Resistin (RETN) Protein |
|
20-abx651562 |
Abbexa |
-
EUR 1228.80
-
EUR 427.20
-
EUR 4051.20
-
EUR 1479.60
-
EUR 844.80
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
|
Human Resistin (RETN) Protein |
|
abx670217-25ug |
Abbexa |
25 ug |
EUR 661.2 |
|
|
 Resistin |
|
cyt-456 |
ProSpec Tany |
5µg |
EUR 60 |
|
Description: Recombinant Human Resistin |
 CLIA Kit) Human Resistin (RETN) CLIA Kit |
|
abx195057-96tests |
Abbexa |
96 tests |
EUR 990 |
|
|
Human Resistin (RETN) CLIA Kit |
|
20-abx492128 |
Abbexa |
-
EUR 8853.60
-
EUR 4719.60
-
EUR 1093.20
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
|
 ELISA kit for Human Resistin |
|
KET6029-48T |
Abbkine |
48T |
EUR 242.4 |
|
|
|
Description: Quantitative sandwich ELISA for measuring Human Resistin in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Resistin |
|
KET6029-5platesof96wells |
Abbkine |
5 plates of 96 wells |
EUR 1292.4 |
|
|
|
Description: Quantitative sandwich ELISA for measuring Human Resistin in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Resistin |
|
KET6029-96T |
Abbkine |
96T |
EUR 346.8 |
|
|
|
Description: Quantitative sandwich ELISA for measuring Human Resistin in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Resistin |
|
EK2351 |
SAB |
96 tests |
EUR 542.4 |
|
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Resistin in samples from serum, plasma, tissue homogenates and other biological fluids. |
 Resistin antibody |
|
10R-R122A |
Fitzgerald |
500 ug |
EUR 327.6 |
|
Description: Mouse monoclonal Resistin antibody |
Resistin antibody |
|
20R-1870 |
Fitzgerald |
100 ug |
EUR 807.6 |
|
Description: Rabbit polyclonal Resistin antibody |
Resistin antibody |
|
20R-1872 |
Fitzgerald |
100 ug |
EUR 807.6 |
|
Description: Rabbit polyclonal Resistin antibody |
Resistin antibody |
|
70R-RG001 |
Fitzgerald |
100 ug |
EUR 418.8 |
|
Description: Affinity purified Goat polyclonal Resistin antibody |
Resistin antibody |
|
70R-RG003 |
Fitzgerald |
50 ug |
EUR 327.6 |
|
Description: Affinity purified Goat polyclonal Resistin antibody |
Resistin antibody |
|
70R-RG005 |
Fitzgerald |
50 ug |
EUR 327.6 |
|
Description: Affinity purified Goat polyclonal Resistin antibody |
Resistin antibody |
|
70R-RR018 |
Fitzgerald |
100 ug |
EUR 360 |
|
Description: Affinity purified Rabbit polyclonal Resistin antibody |
 Resistin Antibody |
|
5997-100 |
Biovision |
each |
EUR 379.2 |
Resistin Antibody |
|
5997-30T |
Biovision |
each |
EUR 175.2 |
Resistin Antibody |
|
5999-100 |
Biovision |
each |
EUR 379.2 |
Resistin Antibody |
|
5999-30T |
Biovision |
each |
EUR 175.2 |
Resistin Antibody |
|
5561-100 |
Biovision |
each |
EUR 379.2 |
Resistin Antibody |
|
5561-30T |
Biovision |
each |
EUR 175.2 |
Resistin antibody |
|
70R-12369 |
Fitzgerald |
100 ug |
EUR 483.6 |
|
Description: Rabbit polyclonal Resistin antibody |
Resistin antibody |
|
70R-12461 |
Fitzgerald |
100 ug |
EUR 483.6 |
|
Description: Rabbit polyclonal Resistin antibody |
Resistin antibody |
|
70R-12463 |
Fitzgerald |
100 ug |
EUR 483.6 |
|
Description: Rabbit polyclonal Resistin antibody |
Resistin antibody |
|
70R-14012 |
Fitzgerald |
100 ug |
EUR 386.4 |
|
Description: Affinity purified Rabbit polyclonal Resistin antibody |
Resistin antibody |
|
70R-51393 |
Fitzgerald |
100 ul |
EUR 292.8 |
|
Description: Purified Polyclonal Resistin antibody |
Resistin Antibody |
|
R30545 |
NSJ Bioreagents |
100 ug |
EUR 356.15 |
|
Description: Resistin, also known as adipose tissue-specific secretory factor(ADSF) or C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein(XCP1) is a cysteine-rich protein that in humans is encoded by the RETN gene. The resistin gene comprises 4 exons, the first of which is untranslated, and spans approximately 1,750 bp. The human resistin gene is localized to a cloned fragment of human chromosome 19. In primates, pigs and dogs, resistin is secreted by immune and epithelial cells while in rodents, it is secreted by adipose tissue. Resistin is a cytokine whose physiologic role has been the subject of much controversy regarding its involvement with obesity and type II diabetes mellitus. |
Resistin Antibody |
|
R32326 |
NSJ Bioreagents |
100 ug |
EUR 356.15 |
|
Description: Resistin, also known as adipose tissue-specific secretory factor (ADSF) or C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein (XCP1) is a cysteine-rich protein that in humans is encoded by the RETN gene. The resistin gene comprises 4 exons, the first of which is untranslated, and spans approximately 1,750 bp. The human resistin gene is localized to a cloned fragment of human chromosome 19. In primates, pigs and dogs, resistin is secreted by immune and epithelial cells while in rodents, it is secreted by adipose tissue. Resistin is a cytokine whose physiologic role has been the subject of much controversy regarding its involvement with obesity and type II diabetes mellitus. |
Resistin Antibody |
|
E306858 |
EnoGene |
200ul |
EUR 275 |
|
Description: Available in various conjugation types. |
 ELISA Kit) Human Resistin (RETN) ELISA Kit |
|
abx252068-96tests |
Abbexa |
96 tests |
EUR 764.4 |
|
|
Human Resistin (RETN) ELISA Kit |
|
abx050199-96tests |
Abbexa |
96 tests |
EUR 801.6 |
|
|
Human Resistin (RETN) ELISA Kit |
|
abx051478-96tests |
Abbexa |
96 tests |
EUR 801.6 |
|
|
Human Resistin (RETN) ELISA Kit |
|
abx575492-96tests |
Abbexa |
96 tests |
EUR 764.4 |
|
|
 Human Resistin, RETN ELISA Kit |
|
DEIA139 |
Creative Diagnostics |
15 Plates |
EUR 2889.6 |
|
Description: Human RETN ELISA development kit contains the key components required for the quantitative measurement of natural and/or recombinant RETN in a sandwich ELISA format within the range of 16-2,000 pg/mL. Using the ELISA protocol described below, this kit provides sufficient reagents to assay RETN in approximately 1,500 ELISA plate wells. |
Human Resistin, RETN ELISA Kit |
|
DEIA221 |
Creative Diagnostics |
96T |
EUR 1020 |
|
Description: This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). It is developed for quantitative measurement of Human RETN in serum, plasma and other biological fluids. |
Human Resistin (RETN) ELISA Kit |
|
DLR-RETN-Hu-48T |
DL Develop |
48T |
EUR 529.2 |
|
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Resistin (RETN) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Human Resistin (RETN) ELISA Kit |
|
DLR-RETN-Hu-96T |
DL Develop |
96T |
EUR 684 |
|
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Resistin (RETN) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Human Resistin (RETN) ELISA Kit |
|
20-abx152952 |
Abbexa |
-
EUR 6768.00
-
EUR 3610.80
-
EUR 848.40
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
|
Human Resistin (RETN) ELISA Kit |
|
RD-RETN-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 523.2 |
Human Resistin (RETN) ELISA Kit |
|
RD-RETN-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 721.2 |
Human Resistin (RETN) ELISA Kit |
|
RDR-RETN-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 546 |
Human Resistin (RETN) ELISA Kit |
|
RDR-RETN-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 754.8 |
Human Resistin (RETN) ELISA Kit |
|
RDR-RETN-Hu-48T |
Reddot Biotech |
48T |
EUR 389.55 |
|
|
|
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. |
Human Resistin (RETN) ELISA Kit |
|
RDR-RETN-Hu-96T |
Reddot Biotech |
96T |
EUR 556.5 |
|
|
|
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. |
Human Resistin (RETN) ELISA Kit |
|
RD-RETN-Hu-48T |
Reddot Biotech |
48T |
EUR 371 |
|
|
|
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. |
Human Resistin (RETN) ELISA Kit |
|
RD-RETN-Hu-96T |
Reddot Biotech |
96T |
EUR 530 |
|
|
|
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. |
Human Resistin (RETN) ELISA Kit |
|
SEA847Hu-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 4091.29 |
|
|
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Resistin (RETN) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
Human Resistin (RETN) ELISA Kit |
|
SEA847Hu-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 442.1 |
|
|
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Resistin (RETN) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
Human Resistin (RETN) ELISA Kit |
|
SEA847Hu-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 580.15 |
|
|
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Resistin (RETN) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
Human Resistin (RETN) ELISA Kit |
|
SEA847Hu-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 2250.68 |
|
|
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Resistin (RETN) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
Human Resistin (RETN) ELISA Kit |
|
4-SEA847Hu |
Cloud-Clone |
-
EUR 4152.00
-
EUR 2191.20
-
EUR 580.80
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
|
|
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Resistin (RETN) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. |
Human Resistin (RETN) ELISA Kit |
|
KTE60804-96T5 |
Abbkine |
96 T×5 |
EUR 2809 |
|
Description: Human Resistin (RETN) ELISA Kit employs a two-site sandwich ELISA to quantitate Resistin in samples. |
Human Resistin (RETN) ELISA Kit |
|
KTE60804-96T50 |
Abbkine |
96 T×50 |
EUR 26209 |
|
Description: Human Resistin (RETN) ELISA Kit employs a two-site sandwich ELISA to quantitate Resistin in samples. |
Human Resistin (RETN) ELISA Kit |
|
DL-RETN-Hu |
DL Develop |
96T |
EUR 362 |
|
|
|
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Human Resistin (RETN) ELISA Kit |
|
DLR-RETN-Hu |
DL Develop |
96T |
EUR 380 |
|
|
|
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
 ELISA Kit) Human Resistin (ADSF) ELISA Kit |
|
IHURETNKT |
Innovative research |
each |
EUR 756 |
|
|
|
Description: Human Resistin (ADSF) ELISA Kit |
Human Resistin (RETN) ELISA Kit |
|
RK10090 |
Abclonal |
96T |
EUR 280 |
 ELISA Kit) Human Resistin(RETN) ELISA Kit |
|
NSL1692Hu |
Sunlong |
96 wells |
EUR 468 |
|
|
) Human Resistin ELISA kit (4X96T) |
|
LF-EK50442 |
Abfrontier |
4×96T |
EUR 2690.4 |
 Recombinant Human Resistin Mutant |
|
7-01525 |
CHI Scientific |
2µg |
Ask for price |
Recombinant Human Resistin Mutant |
|
7-01526 |
CHI Scientific |
10µg |
Ask for price |
Coagulase-negative Staphylococcus (cons) is the most common organism cultured (n = 430). Pseudomonas aeruginosa is a gram-negative organisms most frequently identified (n = 189). In total, 6.9% (n = 83) cultured organism is a fungus. In all, 1.4% (n = 17) is Acanthamoeba. No statistically significant trend in the organism were observed during the study period. sensitivity testing confirmed reasonable sensitivity towards empirical antibiotics used in clinical practice.This is the first report on the trend of microbial keratitis in the South West of England. virulent organisms that may be detected in the culture directly, whereas a low virulent organisms such as the cons are more likely to be detected in enrichment only. Antibiotic sensitivity testing confirmed fluoroquinolone monotherapy as first-line treatment accordingly.
