Campylobacter growth rates in four different matrices: broiler caecal material, live birds, Bolton broth, and brain heart infusion broth.
The purpose of this study was to characterize the growth of Campylobacter in broth enrichment (Bolton broth, brain heart infusion broth), material cecum (in vitro), and in broiler live naturally infected (in vivo) in terms of lag period the mean and time generation and The maximum growth rate and population (cell concentration) is reached.
Bolton and brain heart infusion broth and recovered materials cecum poultry inoculated with 10 strains of Campylobacter (eight Campylobacter jejuni and Campylobacter coli two), incubated in microaerobic conditions, and the concentration of Campylobacter determined periodically using ISO 10 272: 2006. Methods caeca than 10 flock, which infected in the first thinning, is used to characterize the growth of Campylobacter in live birds. mean generation time (G) (initial lag phase to exponential) is calculated using the formula: G = t / 3.3 logb / B. Mean time lag and μmax calculated using Micro Fit (©) Software (Version 1.0, the Institute of Food Research). Statistical comparison is done by using GENSTAT ver. 14.1 (VSN International Ltd., Hemel Hempstead, UK).
Lag period meant Bolton broth, brain heart infusion broth, cecum material, and the live bird was estimated to be 6.6, 6.7, 12.6, and 31.3 hours, respectively. The corresponding mean generation times of 2.1, 2.2, 3.1, and 6.7 hours, respectively; The maximum growth rate was 0.7, 0.8, 0.4, and 2 generations h (-1) and the maximum population obtained in each matrix were 9.6, 9.9, 7.8, and 7.4 log10 CFU / g, respectively.
This study provides data on the growth of Campylobacter in various media lab, the contents of the cecum, and broiler can be used to develop predictive models and / or inform the control strategy based on science as the maximum time between testing herd and slaughter, slaughter logistics, and one stage depopulation unit broiler.
Analysis of 11 years of microbial keratitis in the South West of England using a brain-heart infusion broth.
The purpose of this study was to identify the organisms responsible for microbial keratitis, as identified by the friction of the cornea using a brain-heart infusion broth, trends over time and antimicrobial susceptibility, over a period of 11 years in the two units of the South West of England; Bristol Eye Hospital and the Royal United Hospital, corneal scratches Bath.All done and sent for microbiological analysis between 4 April 2006 and October 31, 2017 in the two units of the retrospective review.
The first line treatment is monotherapy with levofloxacin 0.5% and second-line treatment is a combination of cefuroxime 5% and 1.5% gentamicin. Both direct and enriching culture used.In total, 2614 scratch the cornea of 2116 patients (1082 women, average age 47.7 ± 21.2 years) were identified. 38.1% (n = 996) were positive cultures and organism cultured in 1195. In all, 91.6% is bacteria (69.4% were gram-positive, gram negative 30.6%).Product not found
Coagulase-negative Staphylococcus (cons) is the most common organism cultured (n = 430). Pseudomonas aeruginosa is a gram-negative organisms most frequently identified (n = 189). In total, 6.9% (n = 83) cultured organism is a fungus. In all, 1.4% (n = 17) is Acanthamoeba. No statistically significant trend in the organism were observed during the study period. sensitivity testing confirmed reasonable sensitivity towards empirical antibiotics used in clinical practice.This is the first report on the trend of microbial keratitis in the South West of England. virulent organisms that may be detected in the culture directly, whereas a low virulent organisms such as the cons are more likely to be detected in enrichment only. Antibiotic sensitivity testing confirmed fluoroquinolone monotherapy as first-line treatment accordingly.